Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fbioe.2020.631284 http://hdl.handle.net/11449/210649 |
Resumo: | Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1-46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34 degrees C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a biorefinery concept with value-added green chemicals for the sugarcane productive chain with additional ecologic benefits into a sustainable model. |
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Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery ConceptbioplasticbyproductmolassesvinassebagassePolyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1-46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34 degrees C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a biorefinery concept with value-added green chemicals for the sugarcane productive chain with additional ecologic benefits into a sustainable model.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Inst Res Bioenergy, Rio Claro, BrazilFed Univ Tocantins, Grad Program Food Sci & Technol, Palmas, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, BrazilFed Univ Tocantins, Grad Program Food Sci & Technol, Gurupi, BrazilSao Paulo State Univ, Inst Res Bioenergy, Rio Claro, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, BrazilFrontiers Media SaUniversidade Estadual Paulista (Unesp)Fed Univ TocantinsPaula, Carolina Bilia Chimello de [UNESP]Paula-Elias, Fabricio Coutinho deRodrigues, Marcela Nogueira [UNESP]Coelho, Luciana Fontes [UNESP]Oliveira, Nayra Morgana Lima deAlmeida, Alex Fernando deContiero, Jonas [UNESP]2021-06-26T01:23:14Z2021-06-26T01:23:14Z2021-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.3389/fbioe.2020.631284Frontiers In Bioengineering And Biotechnology. Lausanne: Frontiers Media Sa, v. 8, 14 p., 2021.2296-4185http://hdl.handle.net/11449/21064910.3389/fbioe.2020.631284WOS:000611992900001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Bioengineering And Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T22:13:37Zoai:repositorio.unesp.br:11449/210649Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T22:13:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
title |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
spellingShingle |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept Paula, Carolina Bilia Chimello de [UNESP] bioplastic byproduct molasses vinasse bagasse |
title_short |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
title_full |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
title_fullStr |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
title_full_unstemmed |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
title_sort |
Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept |
author |
Paula, Carolina Bilia Chimello de [UNESP] |
author_facet |
Paula, Carolina Bilia Chimello de [UNESP] Paula-Elias, Fabricio Coutinho de Rodrigues, Marcela Nogueira [UNESP] Coelho, Luciana Fontes [UNESP] Oliveira, Nayra Morgana Lima de Almeida, Alex Fernando de Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Paula-Elias, Fabricio Coutinho de Rodrigues, Marcela Nogueira [UNESP] Coelho, Luciana Fontes [UNESP] Oliveira, Nayra Morgana Lima de Almeida, Alex Fernando de Contiero, Jonas [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fed Univ Tocantins |
dc.contributor.author.fl_str_mv |
Paula, Carolina Bilia Chimello de [UNESP] Paula-Elias, Fabricio Coutinho de Rodrigues, Marcela Nogueira [UNESP] Coelho, Luciana Fontes [UNESP] Oliveira, Nayra Morgana Lima de Almeida, Alex Fernando de Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
bioplastic byproduct molasses vinasse bagasse |
topic |
bioplastic byproduct molasses vinasse bagasse |
description |
Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1-46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34 degrees C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a biorefinery concept with value-added green chemicals for the sugarcane productive chain with additional ecologic benefits into a sustainable model. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-26T01:23:14Z 2021-06-26T01:23:14Z 2021-01-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fbioe.2020.631284 Frontiers In Bioengineering And Biotechnology. Lausanne: Frontiers Media Sa, v. 8, 14 p., 2021. 2296-4185 http://hdl.handle.net/11449/210649 10.3389/fbioe.2020.631284 WOS:000611992900001 |
url |
http://dx.doi.org/10.3389/fbioe.2020.631284 http://hdl.handle.net/11449/210649 |
identifier_str_mv |
Frontiers In Bioengineering And Biotechnology. Lausanne: Frontiers Media Sa, v. 8, 14 p., 2021. 2296-4185 10.3389/fbioe.2020.631284 WOS:000611992900001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Bioengineering And Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046746339672064 |