Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00203-016-1290-9 http://hdl.handle.net/11449/178282 |
Resumo: | Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri. |
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Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil16S rDNAAntimicrobialBacterial communityLuidia senegalensisMarine bacteriaOlivancillaria urceusShrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.Atlantic Canada Opportunities AgencyCanada Foundation for InnovationCanada Research ChairsFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Natural Sciences and Engineering Research Council of CanadaBiosciences Institute S�o Paulo State University - UNESP, Coastal Campus, Pra�a Infante Dom Henrique s/n, P.O. Box 73601Chemistry Institute S�o Paulo State University – UNESP, Araraquara Campus. Prof. Francisco Degni, 55Department of Chemistry University of Prince Edward Island, 550 University AvenueDepartment of Biomedical Sciences Atlantic Veterinary College University of Prince Edward Island, 550 University AvenueBiosciences Institute S�o Paulo State University - UNESP, Coastal Campus, Pra�a Infante Dom Henrique s/n, P.O. Box 73601Chemistry Institute S�o Paulo State University – UNESP, Araraquara Campus. Prof. Francisco Degni, 55FAPESP: 2012/19419-9FAPESP: 2014/08787-0Universidade Estadual Paulista (Unesp)University of Prince Edward IslandTangerina, Marcelo M. P. [UNESP]Correa, HebelinHaltli, BradVilegas, Wagner [UNESP]Kerr, Russell G.2018-12-11T17:29:37Z2018-12-11T17:29:37Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article155-169application/pdfhttp://dx.doi.org/10.1007/s00203-016-1290-9Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017.1432-072X0302-8933http://hdl.handle.net/11449/17828210.1007/s00203-016-1290-92-s2.0-849883652302-s2.0-84988365230.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Microbiology0,644info:eu-repo/semantics/openAccess2024-01-22T06:25:54Zoai:repositorio.unesp.br:11449/178282Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:41:21.263710Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
title |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
spellingShingle |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil Tangerina, Marcelo M. P. [UNESP] 16S rDNA Antimicrobial Bacterial community Luidia senegalensis Marine bacteria Olivancillaria urceus |
title_short |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
title_full |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
title_fullStr |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
title_full_unstemmed |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
title_sort |
Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil |
author |
Tangerina, Marcelo M. P. [UNESP] |
author_facet |
Tangerina, Marcelo M. P. [UNESP] Correa, Hebelin Haltli, Brad Vilegas, Wagner [UNESP] Kerr, Russell G. |
author_role |
author |
author2 |
Correa, Hebelin Haltli, Brad Vilegas, Wagner [UNESP] Kerr, Russell G. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Prince Edward Island |
dc.contributor.author.fl_str_mv |
Tangerina, Marcelo M. P. [UNESP] Correa, Hebelin Haltli, Brad Vilegas, Wagner [UNESP] Kerr, Russell G. |
dc.subject.por.fl_str_mv |
16S rDNA Antimicrobial Bacterial community Luidia senegalensis Marine bacteria Olivancillaria urceus |
topic |
16S rDNA Antimicrobial Bacterial community Luidia senegalensis Marine bacteria Olivancillaria urceus |
description |
Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:29:37Z 2018-12-11T17:29:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00203-016-1290-9 Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017. 1432-072X 0302-8933 http://hdl.handle.net/11449/178282 10.1007/s00203-016-1290-9 2-s2.0-84988365230 2-s2.0-84988365230.pdf |
url |
http://dx.doi.org/10.1007/s00203-016-1290-9 http://hdl.handle.net/11449/178282 |
identifier_str_mv |
Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017. 1432-072X 0302-8933 10.1007/s00203-016-1290-9 2-s2.0-84988365230 2-s2.0-84988365230.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Microbiology 0,644 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
155-169 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129543786987520 |