Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil

Detalhes bibliográficos
Autor(a) principal: Tangerina, Marcelo M. P. [UNESP]
Data de Publicação: 2017
Outros Autores: Correa, Hebelin, Haltli, Brad, Vilegas, Wagner [UNESP], Kerr, Russell G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00203-016-1290-9
http://hdl.handle.net/11449/178282
Resumo: Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.
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spelling Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil16S rDNAAntimicrobialBacterial communityLuidia senegalensisMarine bacteriaOlivancillaria urceusShrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.Atlantic Canada Opportunities AgencyCanada Foundation for InnovationCanada Research ChairsFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Natural Sciences and Engineering Research Council of CanadaBiosciences Institute S�o Paulo State University - UNESP, Coastal Campus, Pra�a Infante Dom Henrique s/n, P.O. Box 73601Chemistry Institute S�o Paulo State University – UNESP, Araraquara Campus. Prof. Francisco Degni, 55Department of Chemistry University of Prince Edward Island, 550 University AvenueDepartment of Biomedical Sciences Atlantic Veterinary College University of Prince Edward Island, 550 University AvenueBiosciences Institute S�o Paulo State University - UNESP, Coastal Campus, Pra�a Infante Dom Henrique s/n, P.O. Box 73601Chemistry Institute S�o Paulo State University – UNESP, Araraquara Campus. Prof. Francisco Degni, 55FAPESP: 2012/19419-9FAPESP: 2014/08787-0Universidade Estadual Paulista (Unesp)University of Prince Edward IslandTangerina, Marcelo M. P. [UNESP]Correa, HebelinHaltli, BradVilegas, Wagner [UNESP]Kerr, Russell G.2018-12-11T17:29:37Z2018-12-11T17:29:37Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article155-169application/pdfhttp://dx.doi.org/10.1007/s00203-016-1290-9Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017.1432-072X0302-8933http://hdl.handle.net/11449/17828210.1007/s00203-016-1290-92-s2.0-849883652302-s2.0-84988365230.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Microbiology0,644info:eu-repo/semantics/openAccess2024-01-22T06:25:54Zoai:repositorio.unesp.br:11449/178282Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:41:21.263710Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
title Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
spellingShingle Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
Tangerina, Marcelo M. P. [UNESP]
16S rDNA
Antimicrobial
Bacterial community
Luidia senegalensis
Marine bacteria
Olivancillaria urceus
title_short Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
title_full Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
title_fullStr Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
title_full_unstemmed Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
title_sort Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil
author Tangerina, Marcelo M. P. [UNESP]
author_facet Tangerina, Marcelo M. P. [UNESP]
Correa, Hebelin
Haltli, Brad
Vilegas, Wagner [UNESP]
Kerr, Russell G.
author_role author
author2 Correa, Hebelin
Haltli, Brad
Vilegas, Wagner [UNESP]
Kerr, Russell G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Prince Edward Island
dc.contributor.author.fl_str_mv Tangerina, Marcelo M. P. [UNESP]
Correa, Hebelin
Haltli, Brad
Vilegas, Wagner [UNESP]
Kerr, Russell G.
dc.subject.por.fl_str_mv 16S rDNA
Antimicrobial
Bacterial community
Luidia senegalensis
Marine bacteria
Olivancillaria urceus
topic 16S rDNA
Antimicrobial
Bacterial community
Luidia senegalensis
Marine bacteria
Olivancillaria urceus
description Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21�kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:29:37Z
2018-12-11T17:29:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00203-016-1290-9
Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017.
1432-072X
0302-8933
http://hdl.handle.net/11449/178282
10.1007/s00203-016-1290-9
2-s2.0-84988365230
2-s2.0-84988365230.pdf
url http://dx.doi.org/10.1007/s00203-016-1290-9
http://hdl.handle.net/11449/178282
identifier_str_mv Archives of Microbiology, v. 199, n. 1, p. 155-169, 2017.
1432-072X
0302-8933
10.1007/s00203-016-1290-9
2-s2.0-84988365230
2-s2.0-84988365230.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Microbiology
0,644
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 155-169
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129543786987520

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