Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.jafc.0c01421 http://hdl.handle.net/11449/200386 |
Resumo: | Celiac disease (CD) is a chronic illness characterized by an inflammatory process triggered by gluten protein intake. Recent evidence has suggested that the lower relative abundance of bifidobacteria in the intestinal lumen may be associated with CD. Herein, we assessed the effect of the Bifidobacterium species Bifidobacterium bifidum, Bifidobacterium longum, Bembidion breve, Bifidobacterium animalis alone, and also a Bifidobacterium consortium on the digestion of intact gluten proteins (gliadins and glutenins) and the associated immunomodulatory responses elicited by the resulting peptides. The cytotoxicity and proinflammatory responses were evaluated through the activation of NF-kB p65 and the expression of cytokines TNF-α and IL-1β in Caco-2 cell cultures exposed to gluten-derived peptides. The peptides induced a clear reduction in cytotoxic responses and proinflammatory marker levels compared to the gluten fragments generated during noninoculated gastrointestinal digestion. These results highlight the possible use of probiotics based on bifidobacteria as a prospective treatment for CD. |
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Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responsesbifidobacteriaceliac diseasegluten proteinsgluten-derived peptidesimmunomodulatory responsetoxicityCeliac disease (CD) is a chronic illness characterized by an inflammatory process triggered by gluten protein intake. Recent evidence has suggested that the lower relative abundance of bifidobacteria in the intestinal lumen may be associated with CD. Herein, we assessed the effect of the Bifidobacterium species Bifidobacterium bifidum, Bifidobacterium longum, Bembidion breve, Bifidobacterium animalis alone, and also a Bifidobacterium consortium on the digestion of intact gluten proteins (gliadins and glutenins) and the associated immunomodulatory responses elicited by the resulting peptides. The cytotoxicity and proinflammatory responses were evaluated through the activation of NF-kB p65 and the expression of cytokines TNF-α and IL-1β in Caco-2 cell cultures exposed to gluten-derived peptides. The peptides induced a clear reduction in cytotoxic responses and proinflammatory marker levels compared to the gluten fragments generated during noninoculated gastrointestinal digestion. These results highlight the possible use of probiotics based on bifidobacteria as a prospective treatment for CD.Department of Chemistry Federal University of São Carlos (UFSCar)Center of Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)Department of Genetics and Evolution Biotechnology Graduate Program (PPGBiotec) Federal University of São CarlosCenter of Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)De Almeida, Natália Ellen CastilhoEsteves, Franciele Grego [UNESP]Dos Santos-Pinto, José Roberto Aparecido [UNESP]Peres De Paula, CarlaDa Cunha, Anderson FerreiraMalavazi, IranPalma, Mario Sergio [UNESP]Rodrigues-Filho, Edson2020-12-12T02:05:15Z2020-12-12T02:05:15Z2020-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4485-4492http://dx.doi.org/10.1021/acs.jafc.0c01421Journal of Agricultural and Food Chemistry, v. 68, n. 15, p. 4485-4492, 2020.1520-51180021-8561http://hdl.handle.net/11449/20038610.1021/acs.jafc.0c014212-s2.0-85084398532Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Agricultural and Food Chemistryinfo:eu-repo/semantics/openAccess2021-10-23T12:39:39Zoai:repositorio.unesp.br:11449/200386Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:39:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
title |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
spellingShingle |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses De Almeida, Natália Ellen Castilho bifidobacteria celiac disease gluten proteins gluten-derived peptides immunomodulatory response toxicity |
title_short |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
title_full |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
title_fullStr |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
title_full_unstemmed |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
title_sort |
Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses |
author |
De Almeida, Natália Ellen Castilho |
author_facet |
De Almeida, Natália Ellen Castilho Esteves, Franciele Grego [UNESP] Dos Santos-Pinto, José Roberto Aparecido [UNESP] Peres De Paula, Carla Da Cunha, Anderson Ferreira Malavazi, Iran Palma, Mario Sergio [UNESP] Rodrigues-Filho, Edson |
author_role |
author |
author2 |
Esteves, Franciele Grego [UNESP] Dos Santos-Pinto, José Roberto Aparecido [UNESP] Peres De Paula, Carla Da Cunha, Anderson Ferreira Malavazi, Iran Palma, Mario Sergio [UNESP] Rodrigues-Filho, Edson |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
De Almeida, Natália Ellen Castilho Esteves, Franciele Grego [UNESP] Dos Santos-Pinto, José Roberto Aparecido [UNESP] Peres De Paula, Carla Da Cunha, Anderson Ferreira Malavazi, Iran Palma, Mario Sergio [UNESP] Rodrigues-Filho, Edson |
dc.subject.por.fl_str_mv |
bifidobacteria celiac disease gluten proteins gluten-derived peptides immunomodulatory response toxicity |
topic |
bifidobacteria celiac disease gluten proteins gluten-derived peptides immunomodulatory response toxicity |
description |
Celiac disease (CD) is a chronic illness characterized by an inflammatory process triggered by gluten protein intake. Recent evidence has suggested that the lower relative abundance of bifidobacteria in the intestinal lumen may be associated with CD. Herein, we assessed the effect of the Bifidobacterium species Bifidobacterium bifidum, Bifidobacterium longum, Bembidion breve, Bifidobacterium animalis alone, and also a Bifidobacterium consortium on the digestion of intact gluten proteins (gliadins and glutenins) and the associated immunomodulatory responses elicited by the resulting peptides. The cytotoxicity and proinflammatory responses were evaluated through the activation of NF-kB p65 and the expression of cytokines TNF-α and IL-1β in Caco-2 cell cultures exposed to gluten-derived peptides. The peptides induced a clear reduction in cytotoxic responses and proinflammatory marker levels compared to the gluten fragments generated during noninoculated gastrointestinal digestion. These results highlight the possible use of probiotics based on bifidobacteria as a prospective treatment for CD. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:05:15Z 2020-12-12T02:05:15Z 2020-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.jafc.0c01421 Journal of Agricultural and Food Chemistry, v. 68, n. 15, p. 4485-4492, 2020. 1520-5118 0021-8561 http://hdl.handle.net/11449/200386 10.1021/acs.jafc.0c01421 2-s2.0-85084398532 |
url |
http://dx.doi.org/10.1021/acs.jafc.0c01421 http://hdl.handle.net/11449/200386 |
identifier_str_mv |
Journal of Agricultural and Food Chemistry, v. 68, n. 15, p. 4485-4492, 2020. 1520-5118 0021-8561 10.1021/acs.jafc.0c01421 2-s2.0-85084398532 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4485-4492 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649865452879872 |