Application of synthetic peptides to the diagnosis of neurocysticercosis

• Autor(a) principal: Fleury, A.
• Data de Publicação: 2003
• Outros Autores: Beltran, C., Ferrer, E., Garate, T., Harrison, LJS, Parkhouse, RME, Garcia, E., Fragoso, G., Costa-Cruz, J., Biondi, G., Agapejev, S., Sciutto, E.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1046/j.1360-2276.2003.01132.x; http://hdl.handle.net/11449/34883
• topic: peptides; Taenia solium; neurocysticercosis; diagnosis

We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.