Application of synthetic peptides to the diagnosis of neurocysticercosis

Detalhes bibliográficos
Autor(a) principal: Fleury, A.
Data de Publicação: 2003
Outros Autores: Beltran, C., Ferrer, E., Garate, T., Harrison, LJS, Parkhouse, RME, Garcia, E., Fragoso, G., Costa-Cruz, J., Biondi, G., Agapejev, S., Sciutto, E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1046/j.1360-2276.2003.01132.x
http://hdl.handle.net/11449/34883
Resumo: We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
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spelling Application of synthetic peptides to the diagnosis of neurocysticercosispeptidesTaenia soliumneurocysticercosisdiagnosisWe tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, MexicoInst Nacl Neurol & Neurocirurg, Mexico City 14269, DF, MexicoInst Epidemiol Neurol & Neurol Trop, Limoges, FranceInst Salud Carlos III, Madrid 28220, SpainUniv Edinburgh, Dept Trop Anim Hlth, Sir Alexander Robertson Ctr Trop Vet Med, Easter Bush Vet Ctr, Roslin EH25 9RG, Midlothian, ScotlandInst Gulbenkian Ciências, Oeiras, PortugalUniversidade Federal de Uberlândia (UFU), Parasitol Lab, BR-3840090Z Uberlandia, MG, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecn, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecn, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med, BR-18618000 Botucatu, SP, BrazilBlackwell PublishingUniv Nacl Autonoma MexicoInst Nacl Neurol & NeurocirurgInst Epidemiol Neurol & Neurol TropInst Salud Carlos IIIUniv EdinburghInst Gulbenkian CiênciasUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Fleury, A.Beltran, C.Ferrer, E.Garate, T.Harrison, LJSParkhouse, RMEGarcia, E.Fragoso, G.Costa-Cruz, J.Biondi, G.Agapejev, S.Sciutto, E.2014-05-20T15:24:14Z2014-05-20T15:24:14Z2003-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1124-1130application/pdfhttp://dx.doi.org/10.1046/j.1360-2276.2003.01132.xTropical Medicine & International Health. Oxford: Blackwell Publishing Ltd, v. 8, n. 12, p. 1124-1130, 2003.1360-2276http://hdl.handle.net/11449/3488310.1046/j.1360-2276.2003.01132.xWOS:000186877600012WOS000186877600012.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Medicine & International Health2.5411,731info:eu-repo/semantics/openAccess2024-01-17T06:22:41Zoai:repositorio.unesp.br:11449/34883Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:11:47.797763Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Application of synthetic peptides to the diagnosis of neurocysticercosis
title Application of synthetic peptides to the diagnosis of neurocysticercosis
spellingShingle Application of synthetic peptides to the diagnosis of neurocysticercosis
Fleury, A.
peptides
Taenia solium
neurocysticercosis
diagnosis
title_short Application of synthetic peptides to the diagnosis of neurocysticercosis
title_full Application of synthetic peptides to the diagnosis of neurocysticercosis
title_fullStr Application of synthetic peptides to the diagnosis of neurocysticercosis
title_full_unstemmed Application of synthetic peptides to the diagnosis of neurocysticercosis
title_sort Application of synthetic peptides to the diagnosis of neurocysticercosis
author Fleury, A.
author_facet Fleury, A.
Beltran, C.
Ferrer, E.
Garate, T.
Harrison, LJS
Parkhouse, RME
Garcia, E.
Fragoso, G.
Costa-Cruz, J.
Biondi, G.
Agapejev, S.
Sciutto, E.
author_role author
author2 Beltran, C.
Ferrer, E.
Garate, T.
Harrison, LJS
Parkhouse, RME
Garcia, E.
Fragoso, G.
Costa-Cruz, J.
Biondi, G.
Agapejev, S.
Sciutto, E.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Nacl Autonoma Mexico
Inst Nacl Neurol & Neurocirurg
Inst Epidemiol Neurol & Neurol Trop
Inst Salud Carlos III
Univ Edinburgh
Inst Gulbenkian Ciências
Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fleury, A.
Beltran, C.
Ferrer, E.
Garate, T.
Harrison, LJS
Parkhouse, RME
Garcia, E.
Fragoso, G.
Costa-Cruz, J.
Biondi, G.
Agapejev, S.
Sciutto, E.
dc.subject.por.fl_str_mv peptides
Taenia solium
neurocysticercosis
diagnosis
topic peptides
Taenia solium
neurocysticercosis
diagnosis
description We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
2014-05-20T15:24:14Z
2014-05-20T15:24:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1046/j.1360-2276.2003.01132.x
Tropical Medicine & International Health. Oxford: Blackwell Publishing Ltd, v. 8, n. 12, p. 1124-1130, 2003.
1360-2276
http://hdl.handle.net/11449/34883
10.1046/j.1360-2276.2003.01132.x
WOS:000186877600012
WOS000186877600012.pdf
url http://dx.doi.org/10.1046/j.1360-2276.2003.01132.x
http://hdl.handle.net/11449/34883
identifier_str_mv Tropical Medicine & International Health. Oxford: Blackwell Publishing Ltd, v. 8, n. 12, p. 1124-1130, 2003.
1360-2276
10.1046/j.1360-2276.2003.01132.x
WOS:000186877600012
WOS000186877600012.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tropical Medicine & International Health
2.541
1,731
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1124-1130
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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