Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/543606 http://hdl.handle.net/11449/113497 |
Resumo: | Species of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver. |
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Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling PathwaySpecies of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ UNESP, Fac Sci, Dept Biol Sci, BR-17033360 Bauru, SP, BrazilSao Paulo State Univ UNESP, Dept Phys Educ, BR-17033360 Bauru, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ UNESP, Fac Sci, Dept Biol Sci, BR-17033360 Bauru, SP, BrazilSao Paulo State Univ UNESP, Dept Phys Educ, BR-17033360 Bauru, SP, BrazilHindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Ponce Vareda, Priscilla Maria [UNESP]Saldanha, Luiz Leonardo [UNESP]Paula Camaforte, Nathalia Aparecida de [UNESP]Violato, Natalia Moretti [UNESP]Dokkedal, Anne Lígia [UNESP]Bosqueiro, José Roberto [UNESP]2014-12-03T13:11:45Z2014-12-03T13:11:45Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1155/2014/543606Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 11 p., 2014.1741-427Xhttp://hdl.handle.net/11449/11349710.1155/2014/543606WOS:000335727800001WOS000335727800001.pdf2423477869556138Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccess2024-04-24T18:52:55Zoai:repositorio.unesp.br:11449/113497Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-24T18:52:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
title |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
spellingShingle |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway Ponce Vareda, Priscilla Maria [UNESP] |
title_short |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
title_full |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
title_fullStr |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
title_full_unstemmed |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
title_sort |
Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway |
author |
Ponce Vareda, Priscilla Maria [UNESP] |
author_facet |
Ponce Vareda, Priscilla Maria [UNESP] Saldanha, Luiz Leonardo [UNESP] Paula Camaforte, Nathalia Aparecida de [UNESP] Violato, Natalia Moretti [UNESP] Dokkedal, Anne Lígia [UNESP] Bosqueiro, José Roberto [UNESP] |
author_role |
author |
author2 |
Saldanha, Luiz Leonardo [UNESP] Paula Camaforte, Nathalia Aparecida de [UNESP] Violato, Natalia Moretti [UNESP] Dokkedal, Anne Lígia [UNESP] Bosqueiro, José Roberto [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ponce Vareda, Priscilla Maria [UNESP] Saldanha, Luiz Leonardo [UNESP] Paula Camaforte, Nathalia Aparecida de [UNESP] Violato, Natalia Moretti [UNESP] Dokkedal, Anne Lígia [UNESP] Bosqueiro, José Roberto [UNESP] |
description |
Species of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:11:45Z 2014-12-03T13:11:45Z 2014-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/543606 Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 11 p., 2014. 1741-427X http://hdl.handle.net/11449/113497 10.1155/2014/543606 WOS:000335727800001 WOS000335727800001.pdf 2423477869556138 |
url |
http://dx.doi.org/10.1155/2014/543606 http://hdl.handle.net/11449/113497 |
identifier_str_mv |
Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 11 p., 2014. 1741-427X 10.1155/2014/543606 WOS:000335727800001 WOS000335727800001.pdf 2423477869556138 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine 2.064 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045997404749824 |