Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12866-017-1063-x http://hdl.handle.net/11449/174874 |
Resumo: | Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants' Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant. |
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Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infectionBiofilmIron uptake and metabolismLPS modulationPlants'Oxidative burstXanthomonas, adaptation mechanismsBackground: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants' Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Departamento de Ciências Biológicas (DECBI) Instituto de Ciências Exatas e Biológicas (ICEB) Universidade Federal de Ouro Preto (UFOP)Núcleo de Pesquisas em Ciências Biológicas (NUPEB) Universidade Federal de Ouro PretoDepartamento de Bioquímica (DBq) Instituto de Química (IQ) Universidade Federal Do Rio de Janeiro (UFRJ)Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal UNESP - Universidade Estadual Paulista Departamento de TecnologiaInstituto de Química Universidade Estadual de Campinas (UNICAMP)Departamento de Bioquímica (DB) Instituto de Química (IQ) Universidade de São Paulo (USP)Departamento de Ciências Biológicas (DCB) Universidade Federal de São Paulo (UNIFESP)Biocomplexity Institute Virginia TechFaculdade de Ciências Agrárias e Veterinárias de Jaboticabal UNESP - Universidade Estadual Paulista Departamento de TecnologiaUniversidade Federal de Ouro Preto (UFOP)Universidade Federal de Ouro PretoUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Virginia TechMoreira, Leandro M.Soares, Márcia R.Facincani, Agda P. [UNESP]Ferreira, Cristiano B. [UNESP]Ferreira, Rafael M. [UNESP]Ferro, Maria I. T. [UNESP]Gozzo, Fábio C.Felestrino, Érica B.Assis, Renata A. B.Garcia, Camila Carrião M.Setubal, João C.Ferro, Jesus A. [UNESP]De Oliveira, Julio C.F.2018-12-11T17:13:15Z2018-12-11T17:13:15Z2017-07-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12866-017-1063-xBMC Microbiology, v. 17, n. 1, 2017.1471-2180http://hdl.handle.net/11449/17487410.1186/s12866-017-1063-x2-s2.0-850222245252-s2.0-85022224525.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Microbiology1,242info:eu-repo/semantics/openAccess2024-06-07T15:32:22Zoai:repositorio.unesp.br:11449/174874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:16:27.767536Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
title |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
spellingShingle |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection Moreira, Leandro M. Biofilm Iron uptake and metabolism LPS modulation Plants'Oxidative burst Xanthomonas, adaptation mechanisms |
title_short |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
title_full |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
title_fullStr |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
title_full_unstemmed |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
title_sort |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection |
author |
Moreira, Leandro M. |
author_facet |
Moreira, Leandro M. Soares, Márcia R. Facincani, Agda P. [UNESP] Ferreira, Cristiano B. [UNESP] Ferreira, Rafael M. [UNESP] Ferro, Maria I. T. [UNESP] Gozzo, Fábio C. Felestrino, Érica B. Assis, Renata A. B. Garcia, Camila Carrião M. Setubal, João C. Ferro, Jesus A. [UNESP] De Oliveira, Julio C.F. |
author_role |
author |
author2 |
Soares, Márcia R. Facincani, Agda P. [UNESP] Ferreira, Cristiano B. [UNESP] Ferreira, Rafael M. [UNESP] Ferro, Maria I. T. [UNESP] Gozzo, Fábio C. Felestrino, Érica B. Assis, Renata A. B. Garcia, Camila Carrião M. Setubal, João C. Ferro, Jesus A. [UNESP] De Oliveira, Julio C.F. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Ouro Preto (UFOP) Universidade Federal de Ouro Preto Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Virginia Tech |
dc.contributor.author.fl_str_mv |
Moreira, Leandro M. Soares, Márcia R. Facincani, Agda P. [UNESP] Ferreira, Cristiano B. [UNESP] Ferreira, Rafael M. [UNESP] Ferro, Maria I. T. [UNESP] Gozzo, Fábio C. Felestrino, Érica B. Assis, Renata A. B. Garcia, Camila Carrião M. Setubal, João C. Ferro, Jesus A. [UNESP] De Oliveira, Julio C.F. |
dc.subject.por.fl_str_mv |
Biofilm Iron uptake and metabolism LPS modulation Plants'Oxidative burst Xanthomonas, adaptation mechanisms |
topic |
Biofilm Iron uptake and metabolism LPS modulation Plants'Oxidative burst Xanthomonas, adaptation mechanisms |
description |
Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants' Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-11 2018-12-11T17:13:15Z 2018-12-11T17:13:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12866-017-1063-x BMC Microbiology, v. 17, n. 1, 2017. 1471-2180 http://hdl.handle.net/11449/174874 10.1186/s12866-017-1063-x 2-s2.0-85022224525 2-s2.0-85022224525.pdf |
url |
http://dx.doi.org/10.1186/s12866-017-1063-x http://hdl.handle.net/11449/174874 |
identifier_str_mv |
BMC Microbiology, v. 17, n. 1, 2017. 1471-2180 10.1186/s12866-017-1063-x 2-s2.0-85022224525 2-s2.0-85022224525.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Microbiology 1,242 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
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Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129303335927808 |