Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum

Detalhes bibliográficos
Autor(a) principal: Munhoz, Alexandre Dias
Data de Publicação: 2013
Outros Autores: Patriarca Mineo, Tiago Wilson, Alessi, Antonio Carlos [UNESP], Gomes Lopes, Carlos Wilson, Machado, Rosangela Zacarias [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612013000400018
http://hdl.handle.net/11449/113211
Resumo: The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.
id UNSP_29ed4c730a727aefa56f45cf418c3908
oai_identifier_str oai:repositorio.unesp.br:11449/113211
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninumNeosporosisacute phaseInfectionReal time PCRImmunohistochemistryThe aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa Nacional de Cooperacao Academica - Novas FronteirasUniv Estadual Santa Cruz UESC, Dept Ciencias Agra & Ambientais, Ilheus, BA, BrazilUniv Fed Uberlandia UFU, Inst Ciencias Biomed, Uberlandia, MG, BrazilUniv Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilUniv Fed Rural Rio de Janeiro, Dept Parasitol Anim, Seropedica, RJ, BrazilUniv Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilPrograma Nacional de Cooperacao Academica - Novas Fronteiras1512/2007-NF/CAPESBrazilian Coll Veterinary ParasitologyUniversidade Estadual de Santa Cruz (UESC)Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Universidade Federal Rural do Rio de Janeiro (UFRRJ)Munhoz, Alexandre DiasPatriarca Mineo, Tiago WilsonAlessi, Antonio Carlos [UNESP]Gomes Lopes, Carlos WilsonMachado, Rosangela Zacarias [UNESP]2014-12-03T13:11:30Z2014-12-03T13:11:30Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article565-570application/pdfhttp://dx.doi.org/10.1590/S1984-29612013000400018Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013.1984-2961http://hdl.handle.net/11449/113211S1984-29612013000400018WOS:000330849100018S1984-29612013000400018.pdf3254990612451836Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinária1.090info:eu-repo/semantics/openAccess2024-06-07T13:02:39Zoai:repositorio.unesp.br:11449/113211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:29:05.833865Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
spellingShingle Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
Munhoz, Alexandre Dias
Neosporosis
acute phase
Infection
Real time PCR
Immunohistochemistry
title_short Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title_full Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title_fullStr Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title_full_unstemmed Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title_sort Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
author Munhoz, Alexandre Dias
author_facet Munhoz, Alexandre Dias
Patriarca Mineo, Tiago Wilson
Alessi, Antonio Carlos [UNESP]
Gomes Lopes, Carlos Wilson
Machado, Rosangela Zacarias [UNESP]
author_role author
author2 Patriarca Mineo, Tiago Wilson
Alessi, Antonio Carlos [UNESP]
Gomes Lopes, Carlos Wilson
Machado, Rosangela Zacarias [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Santa Cruz (UESC)
Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (Unesp)
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
dc.contributor.author.fl_str_mv Munhoz, Alexandre Dias
Patriarca Mineo, Tiago Wilson
Alessi, Antonio Carlos [UNESP]
Gomes Lopes, Carlos Wilson
Machado, Rosangela Zacarias [UNESP]
dc.subject.por.fl_str_mv Neosporosis
acute phase
Infection
Real time PCR
Immunohistochemistry
topic Neosporosis
acute phase
Infection
Real time PCR
Immunohistochemistry
description The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
2014-12-03T13:11:30Z
2014-12-03T13:11:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612013000400018
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013.
1984-2961
http://hdl.handle.net/11449/113211
S1984-29612013000400018
WOS:000330849100018
S1984-29612013000400018.pdf
3254990612451836
url http://dx.doi.org/10.1590/S1984-29612013000400018
http://hdl.handle.net/11449/113211
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013.
1984-2961
S1984-29612013000400018
WOS:000330849100018
S1984-29612013000400018.pdf
3254990612451836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária
1.090
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 565-570
application/pdf
dc.publisher.none.fl_str_mv Brazilian Coll Veterinary Parasitology
publisher.none.fl_str_mv Brazilian Coll Veterinary Parasitology
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129208304533504