Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612013000400018 http://hdl.handle.net/11449/113211 |
Resumo: | The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase. |
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Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninumNeosporosisacute phaseInfectionReal time PCRImmunohistochemistryThe aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa Nacional de Cooperacao Academica - Novas FronteirasUniv Estadual Santa Cruz UESC, Dept Ciencias Agra & Ambientais, Ilheus, BA, BrazilUniv Fed Uberlandia UFU, Inst Ciencias Biomed, Uberlandia, MG, BrazilUniv Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilUniv Fed Rural Rio de Janeiro, Dept Parasitol Anim, Seropedica, RJ, BrazilUniv Estadual Julio Mesquita Filho UNESP, Dept Patol Vet, Jaboticabal, SP, BrazilPrograma Nacional de Cooperacao Academica - Novas Fronteiras1512/2007-NF/CAPESBrazilian Coll Veterinary ParasitologyUniversidade Estadual de Santa Cruz (UESC)Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Universidade Federal Rural do Rio de Janeiro (UFRRJ)Munhoz, Alexandre DiasPatriarca Mineo, Tiago WilsonAlessi, Antonio Carlos [UNESP]Gomes Lopes, Carlos WilsonMachado, Rosangela Zacarias [UNESP]2014-12-03T13:11:30Z2014-12-03T13:11:30Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article565-570application/pdfhttp://dx.doi.org/10.1590/S1984-29612013000400018Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013.1984-2961http://hdl.handle.net/11449/113211S1984-29612013000400018WOS:000330849100018S1984-29612013000400018.pdf3254990612451836Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinária1.090info:eu-repo/semantics/openAccess2024-06-07T13:02:39Zoai:repositorio.unesp.br:11449/113211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:29:05.833865Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
spellingShingle |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum Munhoz, Alexandre Dias Neosporosis acute phase Infection Real time PCR Immunohistochemistry |
title_short |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title_full |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title_fullStr |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title_full_unstemmed |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title_sort |
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
author |
Munhoz, Alexandre Dias |
author_facet |
Munhoz, Alexandre Dias Patriarca Mineo, Tiago Wilson Alessi, Antonio Carlos [UNESP] Gomes Lopes, Carlos Wilson Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
Patriarca Mineo, Tiago Wilson Alessi, Antonio Carlos [UNESP] Gomes Lopes, Carlos Wilson Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Santa Cruz (UESC) Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
dc.contributor.author.fl_str_mv |
Munhoz, Alexandre Dias Patriarca Mineo, Tiago Wilson Alessi, Antonio Carlos [UNESP] Gomes Lopes, Carlos Wilson Machado, Rosangela Zacarias [UNESP] |
dc.subject.por.fl_str_mv |
Neosporosis acute phase Infection Real time PCR Immunohistochemistry |
topic |
Neosporosis acute phase Infection Real time PCR Immunohistochemistry |
description |
The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 2014-12-03T13:11:30Z 2014-12-03T13:11:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612013000400018 Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013. 1984-2961 http://hdl.handle.net/11449/113211 S1984-29612013000400018 WOS:000330849100018 S1984-29612013000400018.pdf 3254990612451836 |
url |
http://dx.doi.org/10.1590/S1984-29612013000400018 http://hdl.handle.net/11449/113211 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 22, n. 4, p. 565-570, 2013. 1984-2961 S1984-29612013000400018 WOS:000330849100018 S1984-29612013000400018.pdf 3254990612451836 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária 1.090 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
565-570 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Coll Veterinary Parasitology |
publisher.none.fl_str_mv |
Brazilian Coll Veterinary Parasitology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129208304533504 |