Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum

Detalhes bibliográficos
Autor(a) principal: Munhoz, Alexandre Dias
Data de Publicação: 2013
Outros Autores: Mineo, Tiago Wilson Patriarca, Alessi, Antonio Carlos [UNESP], Lopes, Carlos Wilson Gomes, Machado, Rosangela Zacarias [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/227383
Resumo: The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.
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spelling Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninumAssessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninumAcute phaseImmunohistochemistryInfectionNeosporosisReal-time pcrThe aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.Departamento de Ciências Agrárias e Ambientais Universidade Estadual de Santa Cruz-UESC Campus Soane Nazaré de Andrade, Salobrinho, Ilhéus, BAInstituto de Ciências Biomédicas Universidade Federal de Uberlândia-UFU Campus Umuarama, Uberlândia, MGDepartamento de Patologia Veterinária Universidade Estadual Julio Mesquita Filho-UNESP, Jaboticabal, SPDepartamento de Parasitologia Animal Universidade Federal Rural do Rio de Janeiro-UFRRJ, Seropédica, RJDepartamento de Patologia Veterinária Universidade Estadual Julio Mesquita Filho-UNESP, Jaboticabal, SPUniversidade Estadual de Santa Cruz-UESCUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (UNESP)Universidade Federal Rural do Rio de Janeiro-UFRRJMunhoz, Alexandre DiasMineo, Tiago Wilson PatriarcaAlessi, Antonio Carlos [UNESP]Lopes, Carlos Wilson GomesMachado, Rosangela Zacarias [UNESP]2022-04-29T07:12:55Z2022-04-29T07:12:55Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article565-570Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013.0103-846Xhttp://hdl.handle.net/11449/2273832-s2.0-84890510926Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:57Zoai:repositorio.unesp.br:11449/227383Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:15.138264Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum
title Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
spellingShingle Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
Munhoz, Alexandre Dias
Acute phase
Immunohistochemistry
Infection
Neosporosis
Real-time pcr
title_short Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
title_full Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
title_fullStr Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
title_full_unstemmed Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
title_sort Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
author Munhoz, Alexandre Dias
author_facet Munhoz, Alexandre Dias
Mineo, Tiago Wilson Patriarca
Alessi, Antonio Carlos [UNESP]
Lopes, Carlos Wilson Gomes
Machado, Rosangela Zacarias [UNESP]
author_role author
author2 Mineo, Tiago Wilson Patriarca
Alessi, Antonio Carlos [UNESP]
Lopes, Carlos Wilson Gomes
Machado, Rosangela Zacarias [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Santa Cruz-UESC
Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (UNESP)
Universidade Federal Rural do Rio de Janeiro-UFRRJ
dc.contributor.author.fl_str_mv Munhoz, Alexandre Dias
Mineo, Tiago Wilson Patriarca
Alessi, Antonio Carlos [UNESP]
Lopes, Carlos Wilson Gomes
Machado, Rosangela Zacarias [UNESP]
dc.subject.por.fl_str_mv Acute phase
Immunohistochemistry
Infection
Neosporosis
Real-time pcr
topic Acute phase
Immunohistochemistry
Infection
Neosporosis
Real-time pcr
description The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
2022-04-29T07:12:55Z
2022-04-29T07:12:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013.
0103-846X
http://hdl.handle.net/11449/227383
2-s2.0-84890510926
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013.
0103-846X
2-s2.0-84890510926
url http://hdl.handle.net/11449/227383
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 565-570
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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