Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/227383 |
Resumo: | The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase. |
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Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninumAssessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninumAcute phaseImmunohistochemistryInfectionNeosporosisReal-time pcrThe aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase.Departamento de Ciências Agrárias e Ambientais Universidade Estadual de Santa Cruz-UESC Campus Soane Nazaré de Andrade, Salobrinho, Ilhéus, BAInstituto de Ciências Biomédicas Universidade Federal de Uberlândia-UFU Campus Umuarama, Uberlândia, MGDepartamento de Patologia Veterinária Universidade Estadual Julio Mesquita Filho-UNESP, Jaboticabal, SPDepartamento de Parasitologia Animal Universidade Federal Rural do Rio de Janeiro-UFRRJ, Seropédica, RJDepartamento de Patologia Veterinária Universidade Estadual Julio Mesquita Filho-UNESP, Jaboticabal, SPUniversidade Estadual de Santa Cruz-UESCUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (UNESP)Universidade Federal Rural do Rio de Janeiro-UFRRJMunhoz, Alexandre DiasMineo, Tiago Wilson PatriarcaAlessi, Antonio Carlos [UNESP]Lopes, Carlos Wilson GomesMachado, Rosangela Zacarias [UNESP]2022-04-29T07:12:55Z2022-04-29T07:12:55Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article565-570Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013.0103-846Xhttp://hdl.handle.net/11449/2273832-s2.0-84890510926Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:57Zoai:repositorio.unesp.br:11449/227383Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:15.138264Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum Assessment of experimental infection for dogs using Gallus gallus chorioallantoic membranes inoculated with Neospora caninum |
title |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
spellingShingle |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum Munhoz, Alexandre Dias Acute phase Immunohistochemistry Infection Neosporosis Real-time pcr |
title_short |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
title_full |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
title_fullStr |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
title_full_unstemmed |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
title_sort |
Avaliação da infecção experimental de cães com membranas córion-alantóides de Gallus gallus inoculadas com Neospora caninum |
author |
Munhoz, Alexandre Dias |
author_facet |
Munhoz, Alexandre Dias Mineo, Tiago Wilson Patriarca Alessi, Antonio Carlos [UNESP] Lopes, Carlos Wilson Gomes Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
Mineo, Tiago Wilson Patriarca Alessi, Antonio Carlos [UNESP] Lopes, Carlos Wilson Gomes Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Santa Cruz-UESC Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (UNESP) Universidade Federal Rural do Rio de Janeiro-UFRRJ |
dc.contributor.author.fl_str_mv |
Munhoz, Alexandre Dias Mineo, Tiago Wilson Patriarca Alessi, Antonio Carlos [UNESP] Lopes, Carlos Wilson Gomes Machado, Rosangela Zacarias [UNESP] |
dc.subject.por.fl_str_mv |
Acute phase Immunohistochemistry Infection Neosporosis Real-time pcr |
topic |
Acute phase Immunohistochemistry Infection Neosporosis Real-time pcr |
description |
The aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by Neospora caninum in dogs fed Gallus gallus chorioallantoic membranes (CMs) previously infected in ovo. Five two-month-old pups were used. Each dog was given five CMs that were previously infected with N. caninum via the oral route. Four animals were euthanized in the first week of infection. All four dogs had their stools examined one week prior to and up to the day they were euthanized. The stools of the uneuthanized dog were collected for 30 days. After euthanasia, organ sections were utilized for histopathology, immunohistochemistry, indirect immunofluorescent tissue reactions, PCR and real-time PCR to detect parasites. Necropsy revealed that the small and large intestines, spleen, and lungs were affected. No oocysts or N. caninum DNA were identified in the stool samples. Real-time PCR was the most sensitive technique used to detect the protozoa in tissues, which were identified in 41% of the analyzed samples. Our results indicate that an experimental model using previously infected CMs appears to be a useful model for the study of the host-parasite relationship during the infection's acute phase. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 2022-04-29T07:12:55Z 2022-04-29T07:12:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013. 0103-846X http://hdl.handle.net/11449/227383 2-s2.0-84890510926 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 565-570, 2013. 0103-846X 2-s2.0-84890510926 |
url |
http://hdl.handle.net/11449/227383 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
565-570 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129334845636608 |