Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s1415-47572008000300008 http://hdl.handle.net/11449/225211 |
Resumo: | Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética. |
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Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)CytogeneticsKaryotypeMeiosisNucleolusTriatoma klugiTriatomineTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética.Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Julio de Mesquita Filho, Rua Cristóvão Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Julio de Mesquita Filho, Rua Cristóvão Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPUniversidade Estadual Paulista (UNESP)da Costa, Laiana Cristina [UNESP]de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]Tartarotti, Ester [UNESP]2022-04-28T20:42:15Z2022-04-28T20:42:15Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-444http://dx.doi.org/10.1590/s1415-47572008000300008Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008.1678-46851415-4757http://hdl.handle.net/11449/22521110.1590/s1415-475720080003000082-s2.0-46649097440Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2022-04-28T20:42:15Zoai:repositorio.unesp.br:11449/225211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:53:23.076504Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
spellingShingle |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) da Costa, Laiana Cristina [UNESP] Cytogenetics Karyotype Meiosis Nucleolus Triatoma klugi Triatomine |
title_short |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_full |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_fullStr |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_full_unstemmed |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_sort |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
author |
da Costa, Laiana Cristina [UNESP] |
author_facet |
da Costa, Laiana Cristina [UNESP] de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Tartarotti, Ester [UNESP] |
author_role |
author |
author2 |
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Tartarotti, Ester [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
da Costa, Laiana Cristina [UNESP] de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Tartarotti, Ester [UNESP] |
dc.subject.por.fl_str_mv |
Cytogenetics Karyotype Meiosis Nucleolus Triatoma klugi Triatomine |
topic |
Cytogenetics Karyotype Meiosis Nucleolus Triatoma klugi Triatomine |
description |
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-28T20:42:15Z 2022-04-28T20:42:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s1415-47572008000300008 Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008. 1678-4685 1415-4757 http://hdl.handle.net/11449/225211 10.1590/s1415-47572008000300008 2-s2.0-46649097440 |
url |
http://dx.doi.org/10.1590/s1415-47572008000300008 http://hdl.handle.net/11449/225211 |
identifier_str_mv |
Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008. 1678-4685 1415-4757 10.1590/s1415-47572008000300008 2-s2.0-46649097440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
438-444 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129369855492096 |