Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)

Detalhes bibliográficos
Autor(a) principal: da Costa, Laiana Cristina [UNESP]
Data de Publicação: 2008
Outros Autores: de Azeredo-Oliveira, Maria Tercília Vilela [UNESP], Tartarotti, Ester [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/s1415-47572008000300008
http://hdl.handle.net/11449/225211
Resumo: Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética.
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spelling Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)CytogeneticsKaryotypeMeiosisNucleolusTriatoma klugiTriatomineTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética.Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Julio de Mesquita Filho, Rua Cristóvão Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Julio de Mesquita Filho, Rua Cristóvão Colombo 2265, 15054-000 Sao Jose do Rio Preto, SPUniversidade Estadual Paulista (UNESP)da Costa, Laiana Cristina [UNESP]de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]Tartarotti, Ester [UNESP]2022-04-28T20:42:15Z2022-04-28T20:42:15Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-444http://dx.doi.org/10.1590/s1415-47572008000300008Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008.1678-46851415-4757http://hdl.handle.net/11449/22521110.1590/s1415-475720080003000082-s2.0-46649097440Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2022-04-28T20:42:15Zoai:repositorio.unesp.br:11449/225211Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:53:23.076504Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
spellingShingle Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
da Costa, Laiana Cristina [UNESP]
Cytogenetics
Karyotype
Meiosis
Nucleolus
Triatoma klugi
Triatomine
title_short Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_full Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_fullStr Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_full_unstemmed Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_sort Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
author da Costa, Laiana Cristina [UNESP]
author_facet da Costa, Laiana Cristina [UNESP]
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Tartarotti, Ester [UNESP]
author_role author
author2 de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Tartarotti, Ester [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv da Costa, Laiana Cristina [UNESP]
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Tartarotti, Ester [UNESP]
dc.subject.por.fl_str_mv Cytogenetics
Karyotype
Meiosis
Nucleolus
Triatoma klugi
Triatomine
topic Cytogenetics
Karyotype
Meiosis
Nucleolus
Triatoma klugi
Triatomine
description Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugl and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lactoacetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. Copyright © 2008, Sociedade Brasileira de Genética.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2022-04-28T20:42:15Z
2022-04-28T20:42:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/s1415-47572008000300008
Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008.
1678-4685
1415-4757
http://hdl.handle.net/11449/225211
10.1590/s1415-47572008000300008
2-s2.0-46649097440
url http://dx.doi.org/10.1590/s1415-47572008000300008
http://hdl.handle.net/11449/225211
identifier_str_mv Genetics and Molecular Biology, v. 31, n. 2, p. 438-444, 2008.
1678-4685
1415-4757
10.1590/s1415-47572008000300008
2-s2.0-46649097440
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 438-444
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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