Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)

Detalhes bibliográficos
Autor(a) principal: da Costa, Laiana Cristina [UNESP]
Data de Publicação: 2008
Outros Autores: Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP], Tartarotti, Ester [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572008000300008
http://hdl.handle.net/11449/21510
Resumo: Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.
id UNSP_7e43d730ab1ba28f1d69614fac7d83b3
oai_identifier_str oai:repositorio.unesp.br:11449/21510
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)Triatoma klugiTriatomineCytogeneticNucleolusKaryotypeMeiosisTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, BrazilSoc Brasil GeneticaUniversidade Estadual Paulista (Unesp)da Costa, Laiana Cristina [UNESP]Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP]Tartarotti, Ester [UNESP]2014-05-20T14:00:53Z2014-05-20T14:00:53Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-444application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000300008Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008.1415-4757http://hdl.handle.net/11449/21510S1415-47572008000300008WOS:000256957500008S1415-47572008000300008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-11-02T06:06:10Zoai:repositorio.unesp.br:11449/21510Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:29.470785Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
spellingShingle Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
da Costa, Laiana Cristina [UNESP]
Triatoma klugi
Triatomine
Cytogenetic
Nucleolus
Karyotype
Meiosis
title_short Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_full Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_fullStr Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_full_unstemmed Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
title_sort Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
author da Costa, Laiana Cristina [UNESP]
author_facet da Costa, Laiana Cristina [UNESP]
Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP]
Tartarotti, Ester [UNESP]
author_role author
author2 Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP]
Tartarotti, Ester [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv da Costa, Laiana Cristina [UNESP]
Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP]
Tartarotti, Ester [UNESP]
dc.subject.por.fl_str_mv Triatoma klugi
Triatomine
Cytogenetic
Nucleolus
Karyotype
Meiosis
topic Triatoma klugi
Triatomine
Cytogenetic
Nucleolus
Karyotype
Meiosis
description Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2014-05-20T14:00:53Z
2014-05-20T14:00:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572008000300008
Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008.
1415-4757
http://hdl.handle.net/11449/21510
S1415-47572008000300008
WOS:000256957500008
S1415-47572008000300008.pdf
url http://dx.doi.org/10.1590/S1415-47572008000300008
http://hdl.handle.net/11449/21510
identifier_str_mv Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008.
1415-4757
S1415-47572008000300008
WOS:000256957500008
S1415-47572008000300008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 438-444
application/pdf
dc.publisher.none.fl_str_mv Soc Brasil Genetica
publisher.none.fl_str_mv Soc Brasil Genetica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128686524727296