Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572008000300008 http://hdl.handle.net/11449/21510 |
Resumo: | Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. |
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Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera)Triatoma klugiTriatomineCytogeneticNucleolusKaryotypeMeiosisTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, BrazilSoc Brasil GeneticaUniversidade Estadual Paulista (Unesp)da Costa, Laiana Cristina [UNESP]Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP]Tartarotti, Ester [UNESP]2014-05-20T14:00:53Z2014-05-20T14:00:53Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article438-444application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000300008Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008.1415-4757http://hdl.handle.net/11449/21510S1415-47572008000300008WOS:000256957500008S1415-47572008000300008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-11-02T06:06:10Zoai:repositorio.unesp.br:11449/21510Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:29.470785Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
spellingShingle |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) da Costa, Laiana Cristina [UNESP] Triatoma klugi Triatomine Cytogenetic Nucleolus Karyotype Meiosis |
title_short |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_full |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_fullStr |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_full_unstemmed |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
title_sort |
Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera) |
author |
da Costa, Laiana Cristina [UNESP] |
author_facet |
da Costa, Laiana Cristina [UNESP] Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP] Tartarotti, Ester [UNESP] |
author_role |
author |
author2 |
Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP] Tartarotti, Ester [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Costa, Laiana Cristina [UNESP] Vilela de Azeredo-Oliveira, Maria Tercilia [UNESP] Tartarotti, Ester [UNESP] |
dc.subject.por.fl_str_mv |
Triatoma klugi Triatomine Cytogenetic Nucleolus Karyotype Meiosis |
topic |
Triatoma klugi Triatomine Cytogenetic Nucleolus Karyotype Meiosis |
description |
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T14:00:53Z 2014-05-20T14:00:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572008000300008 Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008. 1415-4757 http://hdl.handle.net/11449/21510 S1415-47572008000300008 WOS:000256957500008 S1415-47572008000300008.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572008000300008 http://hdl.handle.net/11449/21510 |
identifier_str_mv |
Genetics and Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 31, n. 2, p. 438-444, 2008. 1415-4757 S1415-47572008000300008 WOS:000256957500008 S1415-47572008000300008.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
438-444 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasil Genetica |
publisher.none.fl_str_mv |
Soc Brasil Genetica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128686524727296 |