How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00572-019-00917-y http://hdl.handle.net/11449/198164 |
Resumo: | Despite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. A meta-barcoding approach based on Illumina sequencing of internal transcribed spacers (ITS1 and ITS2) was carried out on DNA extracted from root samples (fine roots and ECM root tips separately). ECM fungi dominated the root-associated fungal community, with more than 90% of sequences assigned to the genus Pisolithus. The morphological and barcoding results demonstrated, for the first time, the presence of ECM symbiosis down to 4-m. The molecular diversity of Pisolithus spp. was strongly dependent on depth, with soil pH and soil water content as primary drivers of the Pisolithus spp. structure. Altogether, our results highlight the importance to consider the ECM symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils. |
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How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantationDeep fine rootsDiversityECM root tipsEucalyptus grandisNext-generation sequencingTropical forest plantationsDespite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. A meta-barcoding approach based on Illumina sequencing of internal transcribed spacers (ITS1 and ITS2) was carried out on DNA extracted from root samples (fine roots and ECM root tips separately). ECM fungi dominated the root-associated fungal community, with more than 90% of sequences assigned to the genus Pisolithus. The morphological and barcoding results demonstrated, for the first time, the presence of ECM symbiosis down to 4-m. The molecular diversity of Pisolithus spp. was strongly dependent on depth, with soil pH and soil water content as primary drivers of the Pisolithus spp. structure. Altogether, our results highlight the importance to consider the ECM symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils.Centre de Coopération Internationale en Recherche Agronomique pour le DéveloppementCIRAD UMR Eco&SolsEco&Sols Univ Montpellier CIRAD INRA IRD Montpellier SupAgroESALQ University São PauloCIRAD UMR BGPIBGPI Univ Montpellier CIRAD INRA IRD Montpellier SupAgroCENA University São PauloFederal University of CearáUNESP University São PauloLSTM Univ Montpellier CIRAD INRA IRD Montpellier SupAgroINRA US 1426 GeT-PlaGe GenotoulUNESP University São PauloUMR Eco&SolsMontpellier SupAgroUniversity São PauloUMR BGPIFederal University of CearáUniversidade Estadual Paulista (Unesp)GenotoulRobin, AgnèsPradier, CélineSanguin, HervéMahé, FrédéricLambais, George Rodriguesde Araujo Pereira, Arthur PrudêncioGermon, Amandine [UNESP]Santana, Maiele CintraTisseyre, PierrePablo, Anne-LaureHeuillard, PaulineSauvadet, MarieBouillet, Jean-PierreAndreote, Fernando DiniPlassard, Claudede Moraes Gonçalves, José LéonardoCardoso, Elke Jurandy Bran NogueiraLaclau, Jean-PaulHinsinger, PhilippeJourdan, Christophe2020-12-12T01:05:23Z2020-12-12T01:05:23Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article637-648http://dx.doi.org/10.1007/s00572-019-00917-yMycorrhiza, v. 29, n. 6, p. 637-648, 2019.1432-18900940-6360http://hdl.handle.net/11449/19816410.1007/s00572-019-00917-y2-s2.0-85075244542Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMycorrhizainfo:eu-repo/semantics/openAccess2021-10-23T09:34:06Zoai:repositorio.unesp.br:11449/198164Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T09:34:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
title |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
spellingShingle |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation Robin, Agnès Deep fine roots Diversity ECM root tips Eucalyptus grandis Next-generation sequencing Tropical forest plantations |
title_short |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
title_full |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
title_fullStr |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
title_full_unstemmed |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
title_sort |
How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation |
author |
Robin, Agnès |
author_facet |
Robin, Agnès Pradier, Céline Sanguin, Hervé Mahé, Frédéric Lambais, George Rodrigues de Araujo Pereira, Arthur Prudêncio Germon, Amandine [UNESP] Santana, Maiele Cintra Tisseyre, Pierre Pablo, Anne-Laure Heuillard, Pauline Sauvadet, Marie Bouillet, Jean-Pierre Andreote, Fernando Dini Plassard, Claude de Moraes Gonçalves, José Léonardo Cardoso, Elke Jurandy Bran Nogueira Laclau, Jean-Paul Hinsinger, Philippe Jourdan, Christophe |
author_role |
author |
author2 |
Pradier, Céline Sanguin, Hervé Mahé, Frédéric Lambais, George Rodrigues de Araujo Pereira, Arthur Prudêncio Germon, Amandine [UNESP] Santana, Maiele Cintra Tisseyre, Pierre Pablo, Anne-Laure Heuillard, Pauline Sauvadet, Marie Bouillet, Jean-Pierre Andreote, Fernando Dini Plassard, Claude de Moraes Gonçalves, José Léonardo Cardoso, Elke Jurandy Bran Nogueira Laclau, Jean-Paul Hinsinger, Philippe Jourdan, Christophe |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
UMR Eco&Sols Montpellier SupAgro University São Paulo UMR BGPI Federal University of Ceará Universidade Estadual Paulista (Unesp) Genotoul |
dc.contributor.author.fl_str_mv |
Robin, Agnès Pradier, Céline Sanguin, Hervé Mahé, Frédéric Lambais, George Rodrigues de Araujo Pereira, Arthur Prudêncio Germon, Amandine [UNESP] Santana, Maiele Cintra Tisseyre, Pierre Pablo, Anne-Laure Heuillard, Pauline Sauvadet, Marie Bouillet, Jean-Pierre Andreote, Fernando Dini Plassard, Claude de Moraes Gonçalves, José Léonardo Cardoso, Elke Jurandy Bran Nogueira Laclau, Jean-Paul Hinsinger, Philippe Jourdan, Christophe |
dc.subject.por.fl_str_mv |
Deep fine roots Diversity ECM root tips Eucalyptus grandis Next-generation sequencing Tropical forest plantations |
topic |
Deep fine roots Diversity ECM root tips Eucalyptus grandis Next-generation sequencing Tropical forest plantations |
description |
Despite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. A meta-barcoding approach based on Illumina sequencing of internal transcribed spacers (ITS1 and ITS2) was carried out on DNA extracted from root samples (fine roots and ECM root tips separately). ECM fungi dominated the root-associated fungal community, with more than 90% of sequences assigned to the genus Pisolithus. The morphological and barcoding results demonstrated, for the first time, the presence of ECM symbiosis down to 4-m. The molecular diversity of Pisolithus spp. was strongly dependent on depth, with soil pH and soil water content as primary drivers of the Pisolithus spp. structure. Altogether, our results highlight the importance to consider the ECM symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2020-12-12T01:05:23Z 2020-12-12T01:05:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00572-019-00917-y Mycorrhiza, v. 29, n. 6, p. 637-648, 2019. 1432-1890 0940-6360 http://hdl.handle.net/11449/198164 10.1007/s00572-019-00917-y 2-s2.0-85075244542 |
url |
http://dx.doi.org/10.1007/s00572-019-00917-y http://hdl.handle.net/11449/198164 |
identifier_str_mv |
Mycorrhiza, v. 29, n. 6, p. 637-648, 2019. 1432-1890 0940-6360 10.1007/s00572-019-00917-y 2-s2.0-85075244542 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mycorrhiza |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
637-648 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964678021971968 |