Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0037-86822007000300005 http://hdl.handle.net/11449/69639 |
Resumo: | HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology. |
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Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procariotoCloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic systemgp21Heterologous expressionHTLV-1Recombinant proteinsmembrane proteinmonoclonal antibodyvirus antibodyvirus glycoproteinantigen antibody reactionbiotechnologydiagnostic kitEscherichia coliHuman T cell leukemia virus 1molecular cloningprokaryoteprotein expressionvirus diagnosisAntibodies, MonoclonalCloning, Molecularenv Gene Products, Human Immunodeficiency VirusGene ExpressionGene Products, envGenetic VectorsHTLV-I AntibodiesHuman T-lymphotropic virus 1HumansImmunoblottingPolymerase Chain ReactionProkaryotaHTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.Centro Regional de Hemoterapia Hospital Das Clínicas Universidade de São Paulo, Ribeirão Preto, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SPDepartamento de Neurologia Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SPDepartamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SPCentro Regional de Hemoterapia HCFMRP USP, R. Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Russo-Carbolante, Elisa Maria de SousaPenteado, Flora Cristina Lobo [UNESP]Medeiros, LucieneKashima, SimoneTakayanagui, Oswaldo M.Covas, Dimas Tadeu2014-05-27T11:22:27Z2014-05-27T11:22:27Z2007-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-281application/pdfhttp://dx.doi.org/10.1590/S0037-86822007000300005Revista da Sociedade Brasileira de Medicina Tropical, v. 40, n. 3, p. 277-281, 2007.0037-8682http://hdl.handle.net/11449/6963910.1590/S0037-86822007000300005S0037-86822007000300005WOS:0002480329000052-s2.0-345471613282-s2.0-34547161328.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2024-06-21T15:18:34Zoai:repositorio.unesp.br:11449/69639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:51:37.389463Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto Cloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic system |
title |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
spellingShingle |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto Russo-Carbolante, Elisa Maria de Sousa gp21 Heterologous expression HTLV-1 Recombinant proteins membrane protein monoclonal antibody virus antibody virus glycoprotein antigen antibody reaction biotechnology diagnostic kit Escherichia coli Human T cell leukemia virus 1 molecular cloning prokaryote protein expression virus diagnosis Antibodies, Monoclonal Cloning, Molecular env Gene Products, Human Immunodeficiency Virus Gene Expression Gene Products, env Genetic Vectors HTLV-I Antibodies Human T-lymphotropic virus 1 Humans Immunoblotting Polymerase Chain Reaction Prokaryota |
title_short |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
title_full |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
title_fullStr |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
title_full_unstemmed |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
title_sort |
Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto |
author |
Russo-Carbolante, Elisa Maria de Sousa |
author_facet |
Russo-Carbolante, Elisa Maria de Sousa Penteado, Flora Cristina Lobo [UNESP] Medeiros, Luciene Kashima, Simone Takayanagui, Oswaldo M. Covas, Dimas Tadeu |
author_role |
author |
author2 |
Penteado, Flora Cristina Lobo [UNESP] Medeiros, Luciene Kashima, Simone Takayanagui, Oswaldo M. Covas, Dimas Tadeu |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Russo-Carbolante, Elisa Maria de Sousa Penteado, Flora Cristina Lobo [UNESP] Medeiros, Luciene Kashima, Simone Takayanagui, Oswaldo M. Covas, Dimas Tadeu |
dc.subject.por.fl_str_mv |
gp21 Heterologous expression HTLV-1 Recombinant proteins membrane protein monoclonal antibody virus antibody virus glycoprotein antigen antibody reaction biotechnology diagnostic kit Escherichia coli Human T cell leukemia virus 1 molecular cloning prokaryote protein expression virus diagnosis Antibodies, Monoclonal Cloning, Molecular env Gene Products, Human Immunodeficiency Virus Gene Expression Gene Products, env Genetic Vectors HTLV-I Antibodies Human T-lymphotropic virus 1 Humans Immunoblotting Polymerase Chain Reaction Prokaryota |
topic |
gp21 Heterologous expression HTLV-1 Recombinant proteins membrane protein monoclonal antibody virus antibody virus glycoprotein antigen antibody reaction biotechnology diagnostic kit Escherichia coli Human T cell leukemia virus 1 molecular cloning prokaryote protein expression virus diagnosis Antibodies, Monoclonal Cloning, Molecular env Gene Products, Human Immunodeficiency Virus Gene Expression Gene Products, env Genetic Vectors HTLV-I Antibodies Human T-lymphotropic virus 1 Humans Immunoblotting Polymerase Chain Reaction Prokaryota |
description |
HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-05-01 2014-05-27T11:22:27Z 2014-05-27T11:22:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0037-86822007000300005 Revista da Sociedade Brasileira de Medicina Tropical, v. 40, n. 3, p. 277-281, 2007. 0037-8682 http://hdl.handle.net/11449/69639 10.1590/S0037-86822007000300005 S0037-86822007000300005 WOS:000248032900005 2-s2.0-34547161328 2-s2.0-34547161328.pdf |
url |
http://dx.doi.org/10.1590/S0037-86822007000300005 http://hdl.handle.net/11449/69639 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical, v. 40, n. 3, p. 277-281, 2007. 0037-8682 10.1590/S0037-86822007000300005 S0037-86822007000300005 WOS:000248032900005 2-s2.0-34547161328 2-s2.0-34547161328.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical 1.358 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
277-281 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128573939122176 |