Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto

• Autor(a) principal: Russo-Carbolante, Elisa Maria de Sousa
• Data de Publicação: 2007
• Outros Autores: Penteado, Flora Cristina Lobo [UNESP], Medeiros, Luciene, Kashima, Simone, Takayanagui, Oswaldo M., Covas, Dimas Tadeu
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0037-86822007000300005; http://hdl.handle.net/11449/69639
• topic: gp21; Heterologous expression; HTLV-1; Recombinant proteins; membrane protein; monoclonal antibody; virus antibody; virus glycoprotein; antigen antibody reaction; biotechnology; diagnostic kit; Escherichia coli; Human T cell leukemia virus 1; molecular cloning; prokaryote; protein expression; virus diagnosis; Antibodies, Monoclonal; Cloning, Molecular; env Gene Products, Human Immunodeficiency Virus; Gene Expression; Gene Products, env; Genetic Vectors; HTLV-I Antibodies; Human T-lymphotropic virus 1; Humans; Immunoblotting; Polymerase Chain Reaction; Prokaryota

HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.