Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jece.2021.106779 http://hdl.handle.net/11449/229898 |
Resumo: | In this work we synthesize and characterize AuNPs using two chemical routes: via sodium citrate and via sodium citrate/borohydride. The cytotoxicity of AuNPs was tested by traditional in vitro assays and the in vivo toxicity was evaluated using alternative animals (Galleria mellonella and Caenorhabditis elegans). Avoidance tests with Folsomia candida were carried out to evaluate possible toxic effects in the soil ecosystem. Through Scanning Electron Microscopy and Transmission Electron Microscopy we verified the spherical shape of the AuNPs, with diameters of 34.8 ± 5.5 nm (via sodium citrate) and 7.9 ± 2.2 nm (via sodium citrate/borohydride). The hydrodynamic diameters values were compatible with those obtained microscopically, 30.1 ± 10.1 nm (method A) and 8.3 ± 2.8 nm (method B). Stability of up to 6 months was verified for AuNPs synthesized via sodium citrate, while synthesis via citrate/sodium borohydride was stable for only 1 month. The AuNPs from method A showed better efficacy in the NPs formation with higher absorbance in the plasmonic band at 523 nm, higher concentration and size, spherical shape, homogeneity and stability. The cytotoxicity of the two AuNPs exhibited a slight dose- and size-dependent behavior, with cell viability greater than 80%. The in vivo toxicity in the G. mellonella model showed a survival rate of 100% for 7 days, however, with C. elegans, the percent of survival increased as the concentration of AuNPs decreased. All concentrations of AuNPs, except for the AuNPs synthesized by the citrate pathway (1 mg/kg), showed non-avoidance responses when exposed to contaminated soil. |
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Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammalsAlternative animal modelAvoidance responseGold nanoparticlesNanotoxicityIn this work we synthesize and characterize AuNPs using two chemical routes: via sodium citrate and via sodium citrate/borohydride. The cytotoxicity of AuNPs was tested by traditional in vitro assays and the in vivo toxicity was evaluated using alternative animals (Galleria mellonella and Caenorhabditis elegans). Avoidance tests with Folsomia candida were carried out to evaluate possible toxic effects in the soil ecosystem. Through Scanning Electron Microscopy and Transmission Electron Microscopy we verified the spherical shape of the AuNPs, with diameters of 34.8 ± 5.5 nm (via sodium citrate) and 7.9 ± 2.2 nm (via sodium citrate/borohydride). The hydrodynamic diameters values were compatible with those obtained microscopically, 30.1 ± 10.1 nm (method A) and 8.3 ± 2.8 nm (method B). Stability of up to 6 months was verified for AuNPs synthesized via sodium citrate, while synthesis via citrate/sodium borohydride was stable for only 1 month. The AuNPs from method A showed better efficacy in the NPs formation with higher absorbance in the plasmonic band at 523 nm, higher concentration and size, spherical shape, homogeneity and stability. The cytotoxicity of the two AuNPs exhibited a slight dose- and size-dependent behavior, with cell viability greater than 80%. The in vivo toxicity in the G. mellonella model showed a survival rate of 100% for 7 days, however, with C. elegans, the percent of survival increased as the concentration of AuNPs decreased. All concentrations of AuNPs, except for the AuNPs synthesized by the citrate pathway (1 mg/kg), showed non-avoidance responses when exposed to contaminated soil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP)Institute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP)School of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP)School of Chemistry and Molecular Biosciences The University of QueenslandPostgraduate Program in Agricultural and Natural Ecosystems (PPGEAN) Federal University of Santa Catarina (UFSC)Area of Exact Sciences and Engineering University of Caxias do Sul (UCS)Institute of Exact Sciences Federal University of Southern and Southeastern Pará (UNIFESSPA)School of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP)Institute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP)School of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP)FAPESP: 2016/11836-0FAPESP: 2017/19603-8CNPq: 470261/2012-9Universidade Estadual Paulista (UNESP)The University of QueenslandUniversidade Federal de Santa Catarina (UFSC)University of Caxias do Sul (UCS)Federal University of Southern and Southeastern Pará (UNIFESSPA)Marcelino, Monica Yonashiro [UNESP]Borges, Felipe Azevedo [UNESP]Scorzoni, Liliana [UNESP]de Lacorte Singulani, Junya [UNESP]Garms, Bruna Cambraia [UNESP]Niemeyer, Júlia CarinaGuerra, Nayrim BrizuelaPegorin Brasil, Giovana Sant'Ana [UNESP]Mussagy, Cassamo Ussemane [UNESP]de Oliveira Carvalho, Francisco AdrianoScontri, Mateus [UNESP]Mendes-Giannini, Maria José Soares [UNESP]Fusco-Almeida, Ana Marisa [UNESP]Herculano, Rondinelli Donizetti [UNESP]2022-04-29T08:36:24Z2022-04-29T08:36:24Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jece.2021.106779Journal of Environmental Chemical Engineering, v. 9, n. 6, 2021.2213-3437http://hdl.handle.net/11449/22989810.1016/j.jece.2021.1067792-s2.0-85119183260Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Environmental Chemical Engineeringinfo:eu-repo/semantics/openAccess2022-04-29T08:36:25Zoai:repositorio.unesp.br:11449/229898Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:36:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
title |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
spellingShingle |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals Marcelino, Monica Yonashiro [UNESP] Alternative animal model Avoidance response Gold nanoparticles Nanotoxicity |
title_short |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
title_full |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
title_fullStr |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
title_full_unstemmed |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
title_sort |
Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals |
author |
Marcelino, Monica Yonashiro [UNESP] |
author_facet |
Marcelino, Monica Yonashiro [UNESP] Borges, Felipe Azevedo [UNESP] Scorzoni, Liliana [UNESP] de Lacorte Singulani, Junya [UNESP] Garms, Bruna Cambraia [UNESP] Niemeyer, Júlia Carina Guerra, Nayrim Brizuela Pegorin Brasil, Giovana Sant'Ana [UNESP] Mussagy, Cassamo Ussemane [UNESP] de Oliveira Carvalho, Francisco Adriano Scontri, Mateus [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
author_role |
author |
author2 |
Borges, Felipe Azevedo [UNESP] Scorzoni, Liliana [UNESP] de Lacorte Singulani, Junya [UNESP] Garms, Bruna Cambraia [UNESP] Niemeyer, Júlia Carina Guerra, Nayrim Brizuela Pegorin Brasil, Giovana Sant'Ana [UNESP] Mussagy, Cassamo Ussemane [UNESP] de Oliveira Carvalho, Francisco Adriano Scontri, Mateus [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) The University of Queensland Universidade Federal de Santa Catarina (UFSC) University of Caxias do Sul (UCS) Federal University of Southern and Southeastern Pará (UNIFESSPA) |
dc.contributor.author.fl_str_mv |
Marcelino, Monica Yonashiro [UNESP] Borges, Felipe Azevedo [UNESP] Scorzoni, Liliana [UNESP] de Lacorte Singulani, Junya [UNESP] Garms, Bruna Cambraia [UNESP] Niemeyer, Júlia Carina Guerra, Nayrim Brizuela Pegorin Brasil, Giovana Sant'Ana [UNESP] Mussagy, Cassamo Ussemane [UNESP] de Oliveira Carvalho, Francisco Adriano Scontri, Mateus [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Herculano, Rondinelli Donizetti [UNESP] |
dc.subject.por.fl_str_mv |
Alternative animal model Avoidance response Gold nanoparticles Nanotoxicity |
topic |
Alternative animal model Avoidance response Gold nanoparticles Nanotoxicity |
description |
In this work we synthesize and characterize AuNPs using two chemical routes: via sodium citrate and via sodium citrate/borohydride. The cytotoxicity of AuNPs was tested by traditional in vitro assays and the in vivo toxicity was evaluated using alternative animals (Galleria mellonella and Caenorhabditis elegans). Avoidance tests with Folsomia candida were carried out to evaluate possible toxic effects in the soil ecosystem. Through Scanning Electron Microscopy and Transmission Electron Microscopy we verified the spherical shape of the AuNPs, with diameters of 34.8 ± 5.5 nm (via sodium citrate) and 7.9 ± 2.2 nm (via sodium citrate/borohydride). The hydrodynamic diameters values were compatible with those obtained microscopically, 30.1 ± 10.1 nm (method A) and 8.3 ± 2.8 nm (method B). Stability of up to 6 months was verified for AuNPs synthesized via sodium citrate, while synthesis via citrate/sodium borohydride was stable for only 1 month. The AuNPs from method A showed better efficacy in the NPs formation with higher absorbance in the plasmonic band at 523 nm, higher concentration and size, spherical shape, homogeneity and stability. The cytotoxicity of the two AuNPs exhibited a slight dose- and size-dependent behavior, with cell viability greater than 80%. The in vivo toxicity in the G. mellonella model showed a survival rate of 100% for 7 days, however, with C. elegans, the percent of survival increased as the concentration of AuNPs decreased. All concentrations of AuNPs, except for the AuNPs synthesized by the citrate pathway (1 mg/kg), showed non-avoidance responses when exposed to contaminated soil. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-29T08:36:24Z 2022-04-29T08:36:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jece.2021.106779 Journal of Environmental Chemical Engineering, v. 9, n. 6, 2021. 2213-3437 http://hdl.handle.net/11449/229898 10.1016/j.jece.2021.106779 2-s2.0-85119183260 |
url |
http://dx.doi.org/10.1016/j.jece.2021.106779 http://hdl.handle.net/11449/229898 |
identifier_str_mv |
Journal of Environmental Chemical Engineering, v. 9, n. 6, 2021. 2213-3437 10.1016/j.jece.2021.106779 2-s2.0-85119183260 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Environmental Chemical Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965208921243648 |