An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/36759 |
Resumo: | Gold nanorods (AuNR) have been explored for many applications, including innovative nanomedicines, which also might contribute to its increase in the environment, namely due to inadequate disposable of wastes into aquatic environments. Early-life stages of amphibians are usually aquatic and sensitive to chemical contamination. Accordingly, this study aimed to determine the sublethal effects of CTAB functionalized AuNR on Xenopus laevis tadpoles. As such, tadpoles were exposed to serial concentrations of AuNR for 72 h. A reduction in the rate of feeding (EC50 = 4 μg.L-1), snout to vent growth (EC50 = 5 μg.L-1) and weight gain (EC50 = 6 μg.L-1), was observed for AuNR-exposed tadpoles. Also, tadpoles actively avoided concentrations ≥ 4 μg.L-1 of AuNR, after 12 h of exposure. At the biochemical level, AuNR caused impairments in antioxidant and nervous system related enzymes. Exposure to CTAB alone caused a high mortality. Results indicated that CTAB functionalized AuNR may induce several sublethal effects that may compromise the organism's fitness. Avoidance behavior (which corresponds to the disappearance of organisms, thus, similar to their death), observed at concentrations matching those inducing sublethal effects, suggest that it should be considered in the risk assessment for amphibians. |
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An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevisAmphibiansNanotoxicityFeedingAvoidance behaviorBiomarkersGold nanorods (AuNR) have been explored for many applications, including innovative nanomedicines, which also might contribute to its increase in the environment, namely due to inadequate disposable of wastes into aquatic environments. Early-life stages of amphibians are usually aquatic and sensitive to chemical contamination. Accordingly, this study aimed to determine the sublethal effects of CTAB functionalized AuNR on Xenopus laevis tadpoles. As such, tadpoles were exposed to serial concentrations of AuNR for 72 h. A reduction in the rate of feeding (EC50 = 4 μg.L-1), snout to vent growth (EC50 = 5 μg.L-1) and weight gain (EC50 = 6 μg.L-1), was observed for AuNR-exposed tadpoles. Also, tadpoles actively avoided concentrations ≥ 4 μg.L-1 of AuNR, after 12 h of exposure. At the biochemical level, AuNR caused impairments in antioxidant and nervous system related enzymes. Exposure to CTAB alone caused a high mortality. Results indicated that CTAB functionalized AuNR may induce several sublethal effects that may compromise the organism's fitness. Avoidance behavior (which corresponds to the disappearance of organisms, thus, similar to their death), observed at concentrations matching those inducing sublethal effects, suggest that it should be considered in the risk assessment for amphibians.Elsevier2023-03-30T15:32:39Z2020-12-05T00:00:00Z2020-12-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36759eng0304-389410.1016/j.jhazmat.2020.123237Costa, BrunoQuintaneiro, C.Daniel-da-Silva, Ana L.Trindade, TitoSoares, A. M. V. M.Lopes, Isabelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:10:51Zoai:ria.ua.pt:10773/36759Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:27.755316Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
title |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
spellingShingle |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis Costa, Bruno Amphibians Nanotoxicity Feeding Avoidance behavior Biomarkers |
title_short |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
title_full |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
title_fullStr |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
title_full_unstemmed |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
title_sort |
An integrated approach to assess the sublethal effects of colloidal gold nanorods in tadpoles of Xenopus laevis |
author |
Costa, Bruno |
author_facet |
Costa, Bruno Quintaneiro, C. Daniel-da-Silva, Ana L. Trindade, Tito Soares, A. M. V. M. Lopes, Isabel |
author_role |
author |
author2 |
Quintaneiro, C. Daniel-da-Silva, Ana L. Trindade, Tito Soares, A. M. V. M. Lopes, Isabel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Bruno Quintaneiro, C. Daniel-da-Silva, Ana L. Trindade, Tito Soares, A. M. V. M. Lopes, Isabel |
dc.subject.por.fl_str_mv |
Amphibians Nanotoxicity Feeding Avoidance behavior Biomarkers |
topic |
Amphibians Nanotoxicity Feeding Avoidance behavior Biomarkers |
description |
Gold nanorods (AuNR) have been explored for many applications, including innovative nanomedicines, which also might contribute to its increase in the environment, namely due to inadequate disposable of wastes into aquatic environments. Early-life stages of amphibians are usually aquatic and sensitive to chemical contamination. Accordingly, this study aimed to determine the sublethal effects of CTAB functionalized AuNR on Xenopus laevis tadpoles. As such, tadpoles were exposed to serial concentrations of AuNR for 72 h. A reduction in the rate of feeding (EC50 = 4 μg.L-1), snout to vent growth (EC50 = 5 μg.L-1) and weight gain (EC50 = 6 μg.L-1), was observed for AuNR-exposed tadpoles. Also, tadpoles actively avoided concentrations ≥ 4 μg.L-1 of AuNR, after 12 h of exposure. At the biochemical level, AuNR caused impairments in antioxidant and nervous system related enzymes. Exposure to CTAB alone caused a high mortality. Results indicated that CTAB functionalized AuNR may induce several sublethal effects that may compromise the organism's fitness. Avoidance behavior (which corresponds to the disappearance of organisms, thus, similar to their death), observed at concentrations matching those inducing sublethal effects, suggest that it should be considered in the risk assessment for amphibians. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-05T00:00:00Z 2020-12-05 2023-03-30T15:32:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/36759 |
url |
http://hdl.handle.net/10773/36759 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0304-3894 10.1016/j.jhazmat.2020.123237 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137729822326784 |