Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.33594/000000284 http://hdl.handle.net/11449/205320 |
Resumo: | Background/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling. |
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Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handlingCalcium kineticCardiac dysfunctionInflammationLycopeneObesityPapillary muscleBackground/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Medical School Sao Paulo State University (UNESP)Institute of Biosciences Sao Paulo State University (UNESP)Medical School Sao Paulo State University (UNESP)Institute of Biosciences Sao Paulo State University (UNESP)FAPESP: 2020/12578-0Universidade Estadual Paulista (Unesp)Ferron, Artur Junio Togneri [UNESP]Francisqueti-Ferron, Fabiane Valentini [UNESP]De Almeida Silva, Carol Cristina Vágula [UNESP]Bazan, Silmeia Garcia Zanati [UNESP]De Campos, Dijon Henrique Salomé [UNESP]Garcia, Jéssica Leite [UNESP]Ghiraldeli, Luciana [UNESP]Minatel, Igor Otávio [UNESP]Correa, Camila Renata [UNESP]Moreto, Fernando [UNESP]Ferreira, Ana Lucia Anjos [UNESP]2021-06-25T10:13:21Z2021-06-25T10:13:21Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1013-1025http://dx.doi.org/10.33594/000000284Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020.1421-97781015-8987http://hdl.handle.net/11449/20532010.33594/0000002842-s2.0-85092660703Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T12:31:47Zoai:repositorio.unesp.br:11449/205320Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:52.441875Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
title |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
spellingShingle |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling Ferron, Artur Junio Togneri [UNESP] Calcium kinetic Cardiac dysfunction Inflammation Lycopene Obesity Papillary muscle |
title_short |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
title_full |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
title_fullStr |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
title_full_unstemmed |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
title_sort |
Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling |
author |
Ferron, Artur Junio Togneri [UNESP] |
author_facet |
Ferron, Artur Junio Togneri [UNESP] Francisqueti-Ferron, Fabiane Valentini [UNESP] De Almeida Silva, Carol Cristina Vágula [UNESP] Bazan, Silmeia Garcia Zanati [UNESP] De Campos, Dijon Henrique Salomé [UNESP] Garcia, Jéssica Leite [UNESP] Ghiraldeli, Luciana [UNESP] Minatel, Igor Otávio [UNESP] Correa, Camila Renata [UNESP] Moreto, Fernando [UNESP] Ferreira, Ana Lucia Anjos [UNESP] |
author_role |
author |
author2 |
Francisqueti-Ferron, Fabiane Valentini [UNESP] De Almeida Silva, Carol Cristina Vágula [UNESP] Bazan, Silmeia Garcia Zanati [UNESP] De Campos, Dijon Henrique Salomé [UNESP] Garcia, Jéssica Leite [UNESP] Ghiraldeli, Luciana [UNESP] Minatel, Igor Otávio [UNESP] Correa, Camila Renata [UNESP] Moreto, Fernando [UNESP] Ferreira, Ana Lucia Anjos [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferron, Artur Junio Togneri [UNESP] Francisqueti-Ferron, Fabiane Valentini [UNESP] De Almeida Silva, Carol Cristina Vágula [UNESP] Bazan, Silmeia Garcia Zanati [UNESP] De Campos, Dijon Henrique Salomé [UNESP] Garcia, Jéssica Leite [UNESP] Ghiraldeli, Luciana [UNESP] Minatel, Igor Otávio [UNESP] Correa, Camila Renata [UNESP] Moreto, Fernando [UNESP] Ferreira, Ana Lucia Anjos [UNESP] |
dc.subject.por.fl_str_mv |
Calcium kinetic Cardiac dysfunction Inflammation Lycopene Obesity Papillary muscle |
topic |
Calcium kinetic Cardiac dysfunction Inflammation Lycopene Obesity Papillary muscle |
description |
Background/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:13:21Z 2021-06-25T10:13:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.33594/000000284 Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020. 1421-9778 1015-8987 http://hdl.handle.net/11449/205320 10.33594/000000284 2-s2.0-85092660703 |
url |
http://dx.doi.org/10.33594/000000284 http://hdl.handle.net/11449/205320 |
identifier_str_mv |
Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020. 1421-9778 1015-8987 10.33594/000000284 2-s2.0-85092660703 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1013-1025 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129297076977664 |