Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling

Detalhes bibliográficos
Autor(a) principal: Ferron, Artur Junio Togneri [UNESP]
Data de Publicação: 2020
Outros Autores: Francisqueti-Ferron, Fabiane Valentini [UNESP], De Almeida Silva, Carol Cristina Vágula [UNESP], Bazan, Silmeia Garcia Zanati [UNESP], De Campos, Dijon Henrique Salomé [UNESP], Garcia, Jéssica Leite [UNESP], Ghiraldeli, Luciana [UNESP], Minatel, Igor Otávio [UNESP], Correa, Camila Renata [UNESP], Moreto, Fernando [UNESP], Ferreira, Ana Lucia Anjos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.33594/000000284
http://hdl.handle.net/11449/205320
Resumo: Background/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling.
id UNSP_2d58650e05136a7dae9583f1093959d7
oai_identifier_str oai:repositorio.unesp.br:11449/205320
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handlingCalcium kineticCardiac dysfunctionInflammationLycopeneObesityPapillary muscleBackground/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Medical School Sao Paulo State University (UNESP)Institute of Biosciences Sao Paulo State University (UNESP)Medical School Sao Paulo State University (UNESP)Institute of Biosciences Sao Paulo State University (UNESP)FAPESP: 2020/12578-0Universidade Estadual Paulista (Unesp)Ferron, Artur Junio Togneri [UNESP]Francisqueti-Ferron, Fabiane Valentini [UNESP]De Almeida Silva, Carol Cristina Vágula [UNESP]Bazan, Silmeia Garcia Zanati [UNESP]De Campos, Dijon Henrique Salomé [UNESP]Garcia, Jéssica Leite [UNESP]Ghiraldeli, Luciana [UNESP]Minatel, Igor Otávio [UNESP]Correa, Camila Renata [UNESP]Moreto, Fernando [UNESP]Ferreira, Ana Lucia Anjos [UNESP]2021-06-25T10:13:21Z2021-06-25T10:13:21Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1013-1025http://dx.doi.org/10.33594/000000284Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020.1421-97781015-8987http://hdl.handle.net/11449/20532010.33594/0000002842-s2.0-85092660703Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T12:31:47Zoai:repositorio.unesp.br:11449/205320Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:52.441875Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
title Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
spellingShingle Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
Ferron, Artur Junio Togneri [UNESP]
Calcium kinetic
Cardiac dysfunction
Inflammation
Lycopene
Obesity
Papillary muscle
title_short Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
title_full Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
title_fullStr Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
title_full_unstemmed Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
title_sort Tomato-oleoresin anti-inflammatory effect recovers obesity-induced cardiac dysfunction by modulating myocardial calcium handling
author Ferron, Artur Junio Togneri [UNESP]
author_facet Ferron, Artur Junio Togneri [UNESP]
Francisqueti-Ferron, Fabiane Valentini [UNESP]
De Almeida Silva, Carol Cristina Vágula [UNESP]
Bazan, Silmeia Garcia Zanati [UNESP]
De Campos, Dijon Henrique Salomé [UNESP]
Garcia, Jéssica Leite [UNESP]
Ghiraldeli, Luciana [UNESP]
Minatel, Igor Otávio [UNESP]
Correa, Camila Renata [UNESP]
Moreto, Fernando [UNESP]
Ferreira, Ana Lucia Anjos [UNESP]
author_role author
author2 Francisqueti-Ferron, Fabiane Valentini [UNESP]
De Almeida Silva, Carol Cristina Vágula [UNESP]
Bazan, Silmeia Garcia Zanati [UNESP]
De Campos, Dijon Henrique Salomé [UNESP]
Garcia, Jéssica Leite [UNESP]
Ghiraldeli, Luciana [UNESP]
Minatel, Igor Otávio [UNESP]
Correa, Camila Renata [UNESP]
Moreto, Fernando [UNESP]
Ferreira, Ana Lucia Anjos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ferron, Artur Junio Togneri [UNESP]
Francisqueti-Ferron, Fabiane Valentini [UNESP]
De Almeida Silva, Carol Cristina Vágula [UNESP]
Bazan, Silmeia Garcia Zanati [UNESP]
De Campos, Dijon Henrique Salomé [UNESP]
Garcia, Jéssica Leite [UNESP]
Ghiraldeli, Luciana [UNESP]
Minatel, Igor Otávio [UNESP]
Correa, Camila Renata [UNESP]
Moreto, Fernando [UNESP]
Ferreira, Ana Lucia Anjos [UNESP]
dc.subject.por.fl_str_mv Calcium kinetic
Cardiac dysfunction
Inflammation
Lycopene
Obesity
Papillary muscle
topic Calcium kinetic
Cardiac dysfunction
Inflammation
Lycopene
Obesity
Papillary muscle
description Background/Aims: Considering the importance of inflammation on obesity-related disorders pathogenesis, including cardiac dysfunction, the interest in natural anti-inflammatory therapeutic strategies has emerged. The lycopene is a carotenoid presents in tomato and red fruits that displays anti-inflammatory properties. In this sense, we will evaluate the anti-inflammatory effect of tomato-oleoresin supplementation on obesity- related cardiac dysfunction by modulating myocardial calcium kinetic. Methods: Male Wistar rats were initially randomized into 2 experimental groups: (Control, n= 20) or high sugar- fat diet (HSF, n=20) for 20 weeks. At week 20th, once detected the cardiac dysfunction (cardiac remodeling, systolic and diastolic dysfunction) by echocardiography in HSF group, animals were randomly divided to begin the treatment with tomato-oleoresin, performing 4 groups: Control (n= 10); Control + tomato tomato-oleoresin supplementation (Control + Ly, n= 10); HSF (n= 10) or HSF + tomato tomato-oleoresin supplementation (HSF + Ly, n= 10). Tomato oleoresin was mixed with maize oil equivalent to 10mg lycopene/kg body weight (BW) per day and given orally, by gavage, every morning for a 10-week period. It was analyzed cardiac inflammatory parameters by the enzyme-linked immunosorbent assay (ELISA) and in vivo (echocardiography) and in vitro (studying isolated papillary muscles from the left ventricle) cardiac function. The groups were compared by Two-Way analysis of variance (ANOVA). Results: The HSF diet induced cardiac dysfunction (FS(%) C: 60.4±1.3; C+Ly: 60.9±1.3; HSF: 51.7±1.3; HSF+Ly: 59.4±1.4) and inflammation (TNF-a: C: 1.88±0.41; C+Ly: 1.93±1.01; HSF: 4.58±1.99; HSF+Ly: 2.03±0.55; IL-6: C: 0.58±0.16; C+Ly: 0.40±0.16; HSF: 2.00±0.45; HSF+Ly: 0.53±0.26; MCP-1: C: 0.31±0.08; C+Ly: 0.43±0.22; HSF: 1.54±0.32; HSF+Ly: 0.50±0.16). Tomato-oleoresin supplementation improved cardiac remodeling and dysfunction, cardiac inflammation and myocardial calcium kinetic. Conclusion: the anti-inflammatory effect of tomato-oleoresin supplementation treated the obesity-induced cardiac dysfunction by modulating myocardial calcium handling.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
2021-06-25T10:13:21Z
2021-06-25T10:13:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.33594/000000284
Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020.
1421-9778
1015-8987
http://hdl.handle.net/11449/205320
10.33594/000000284
2-s2.0-85092660703
url http://dx.doi.org/10.33594/000000284
http://hdl.handle.net/11449/205320
identifier_str_mv Cellular Physiology and Biochemistry, v. 54, n. 5, p. 1013-1025, 2020.
1421-9778
1015-8987
10.33594/000000284
2-s2.0-85092660703
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1013-1025
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129297076977664