Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats

Detalhes bibliográficos
Autor(a) principal: Lima-Leopoldo, Ana Paula
Data de Publicação: 2011
Outros Autores: Leopoldo, André S. [UNESP], Sugizaki, Mário M., Bruno, Alessandro [UNESP], Nascimento, André F. [UNESP], Luvizotto, Renata A.M. [UNESP], Junior, Silvio A. Oliveira, Castardeli, Edson, Padovani, Carlos R. [UNESP], Cicogna, Antonio C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0066-782X2011005000061
http://hdl.handle.net/11449/226562
Resumo: Background: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase.
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spelling Myocardial dysfunction and abnormalities in intracellular calcium handling in obese ratsAdrenergicBetaCalcium elevationMyocardial dysfunctionObesityRatsReceptorsBackground: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase.Departamento de Desportos Centro de Educação Física e Desportos UFES-Universidade Federal do Espírito Santo, Botucatu, SPVitória, ES; Departamento de Clínica Médica Faculdade de Medicina, UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPInstituto de Ciências da Saúde UFMT-Universidade Federal de Mato Grosso, Sinop, MTDepartamento de Fisioterapia UFMS-Universidade Federal do Mato Grosso do Sul, Campo Grande, MSDepartamento de Bioestatística Instituto de Biociências UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPVitória, ES; Departamento de Clínica Médica Faculdade de Medicina, UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPDepartamento de Bioestatística Instituto de Biociências UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (UNESP)UFMT-Universidade Federal de Mato GrossoUniversidade Federal de Mato Grosso do Sul (UFMS)Lima-Leopoldo, Ana PaulaLeopoldo, André S. [UNESP]Sugizaki, Mário M.Bruno, Alessandro [UNESP]Nascimento, André F. [UNESP]Luvizotto, Renata A.M. [UNESP]Junior, Silvio A. OliveiraCastardeli, EdsonPadovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2022-04-29T01:14:05Z2022-04-29T01:14:05Z2011-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article232-240http://dx.doi.org/10.1590/S0066-782X2011005000061Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011.0066-782X1678-4170http://hdl.handle.net/11449/22656210.1590/S0066-782X20110050000612-s2.0-80054012501Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:23:33Zoai:repositorio.unesp.br:11449/226562Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
title Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
spellingShingle Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
Lima-Leopoldo, Ana Paula
Adrenergic
Beta
Calcium elevation
Myocardial dysfunction
Obesity
Rats
Receptors
title_short Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
title_full Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
title_fullStr Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
title_full_unstemmed Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
title_sort Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
author Lima-Leopoldo, Ana Paula
author_facet Lima-Leopoldo, Ana Paula
Leopoldo, André S. [UNESP]
Sugizaki, Mário M.
Bruno, Alessandro [UNESP]
Nascimento, André F. [UNESP]
Luvizotto, Renata A.M. [UNESP]
Junior, Silvio A. Oliveira
Castardeli, Edson
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author_role author
author2 Leopoldo, André S. [UNESP]
Sugizaki, Mário M.
Bruno, Alessandro [UNESP]
Nascimento, André F. [UNESP]
Luvizotto, Renata A.M. [UNESP]
Junior, Silvio A. Oliveira
Castardeli, Edson
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Espírito Santo (UFES)
Universidade Estadual Paulista (UNESP)
UFMT-Universidade Federal de Mato Grosso
Universidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.author.fl_str_mv Lima-Leopoldo, Ana Paula
Leopoldo, André S. [UNESP]
Sugizaki, Mário M.
Bruno, Alessandro [UNESP]
Nascimento, André F. [UNESP]
Luvizotto, Renata A.M. [UNESP]
Junior, Silvio A. Oliveira
Castardeli, Edson
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
dc.subject.por.fl_str_mv Adrenergic
Beta
Calcium elevation
Myocardial dysfunction
Obesity
Rats
Receptors
topic Adrenergic
Beta
Calcium elevation
Myocardial dysfunction
Obesity
Rats
Receptors
description Background: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
2022-04-29T01:14:05Z
2022-04-29T01:14:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0066-782X2011005000061
Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011.
0066-782X
1678-4170
http://hdl.handle.net/11449/226562
10.1590/S0066-782X2011005000061
2-s2.0-80054012501
url http://dx.doi.org/10.1590/S0066-782X2011005000061
http://hdl.handle.net/11449/226562
identifier_str_mv Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011.
0066-782X
1678-4170
10.1590/S0066-782X2011005000061
2-s2.0-80054012501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Cardiologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 232-240
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128168916156416