Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0066-782X2011005000061 http://hdl.handle.net/11449/226562 |
Resumo: | Background: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase. |
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Myocardial dysfunction and abnormalities in intracellular calcium handling in obese ratsAdrenergicBetaCalcium elevationMyocardial dysfunctionObesityRatsReceptorsBackground: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase.Departamento de Desportos Centro de Educação Física e Desportos UFES-Universidade Federal do Espírito Santo, Botucatu, SPVitória, ES; Departamento de Clínica Médica Faculdade de Medicina, UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPInstituto de Ciências da Saúde UFMT-Universidade Federal de Mato Grosso, Sinop, MTDepartamento de Fisioterapia UFMS-Universidade Federal do Mato Grosso do Sul, Campo Grande, MSDepartamento de Bioestatística Instituto de Biociências UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPVitória, ES; Departamento de Clínica Médica Faculdade de Medicina, UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPDepartamento de Bioestatística Instituto de Biociências UNESP-Universidade Estadual Paulista Júlio Mesquita Filho, Botucatu, SPUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (UNESP)UFMT-Universidade Federal de Mato GrossoUniversidade Federal de Mato Grosso do Sul (UFMS)Lima-Leopoldo, Ana PaulaLeopoldo, André S. [UNESP]Sugizaki, Mário M.Bruno, Alessandro [UNESP]Nascimento, André F. [UNESP]Luvizotto, Renata A.M. [UNESP]Junior, Silvio A. OliveiraCastardeli, EdsonPadovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2022-04-29T01:14:05Z2022-04-29T01:14:05Z2011-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article232-240http://dx.doi.org/10.1590/S0066-782X2011005000061Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011.0066-782X1678-4170http://hdl.handle.net/11449/22656210.1590/S0066-782X20110050000612-s2.0-80054012501Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:23:33Zoai:repositorio.unesp.br:11449/226562Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
title |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
spellingShingle |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats Lima-Leopoldo, Ana Paula Adrenergic Beta Calcium elevation Myocardial dysfunction Obesity Rats Receptors |
title_short |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
title_full |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
title_fullStr |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
title_full_unstemmed |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
title_sort |
Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats |
author |
Lima-Leopoldo, Ana Paula |
author_facet |
Lima-Leopoldo, Ana Paula Leopoldo, André S. [UNESP] Sugizaki, Mário M. Bruno, Alessandro [UNESP] Nascimento, André F. [UNESP] Luvizotto, Renata A.M. [UNESP] Junior, Silvio A. Oliveira Castardeli, Edson Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author_role |
author |
author2 |
Leopoldo, André S. [UNESP] Sugizaki, Mário M. Bruno, Alessandro [UNESP] Nascimento, André F. [UNESP] Luvizotto, Renata A.M. [UNESP] Junior, Silvio A. Oliveira Castardeli, Edson Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Espírito Santo (UFES) Universidade Estadual Paulista (UNESP) UFMT-Universidade Federal de Mato Grosso Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Lima-Leopoldo, Ana Paula Leopoldo, André S. [UNESP] Sugizaki, Mário M. Bruno, Alessandro [UNESP] Nascimento, André F. [UNESP] Luvizotto, Renata A.M. [UNESP] Junior, Silvio A. Oliveira Castardeli, Edson Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
dc.subject.por.fl_str_mv |
Adrenergic Beta Calcium elevation Myocardial dysfunction Obesity Rats Receptors |
topic |
Adrenergic Beta Calcium elevation Myocardial dysfunction Obesity Rats Receptors |
description |
Background: Several mechanisms have been proposed to contribute to cardiac dysfunction in obesity models, such as alterations in calcium (Ca 2+) handling proteins and β-adrenergic receptors. Nevertheless, the role of these factors in the development of myocardial dysfunction induced by obesity is still not clear. Objective: The purpose of this study was to investigate whether obesity induced by hypercaloric diets results in cardiac dysfunction. Furthermore, it was evaluated whether this functional abnormality in obese rats is related to abnormal Ca 2+ handling and the β-adrenoceptor system. Methods: Male 30-day-old Wistar rats were fed with standard food (C) and a cycle of five hypercaloric diets (Ob) for 15 weeks. Obesity was defined as increases in body fat percentage in rats. Cardiac function was evaluated by isolated analysis of the left ventricle papillary muscle under basal conditions and after inotropic and lusitropic maneuvers. Results: Compared with the control group, the obese rats had increased body fat and glucose intolerance. The muscles of obese rats developed similar baseline data, but the myocardial responsiveness to post-rest contraction stimulus and increased extracellular Ca 2+ were compromised. There were no changes in cardiac function between groups after β-adrenergic stimulation. Conclusion: Obesity promotes cardiac dysfunction related to changes in intracellular Ca 2+ handling. This functional damage is probably caused by reduced cardiac sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) activation via Ca 2+ calmodulin kinase. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 2022-04-29T01:14:05Z 2022-04-29T01:14:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0066-782X2011005000061 Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011. 0066-782X 1678-4170 http://hdl.handle.net/11449/226562 10.1590/S0066-782X2011005000061 2-s2.0-80054012501 |
url |
http://dx.doi.org/10.1590/S0066-782X2011005000061 http://hdl.handle.net/11449/226562 |
identifier_str_mv |
Arquivos Brasileiros de Cardiologia, v. 97, n. 3, p. 232-240, 2011. 0066-782X 1678-4170 10.1590/S0066-782X2011005000061 2-s2.0-80054012501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
232-240 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128168916156416 |