Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.nutres.2018.12.013 http://hdl.handle.net/11449/188921 |
Resumo: | Skeletal muscle is a highly adaptable tissue capable of remodeling when dynamic stress is altered, including changes in mechanical loading and stretch. When muscle is subjected to an unloaded state (e.g., bedrest, immobilization, spaceflight) the resulting loss of muscle cross sectional area (CSA) impairs force production. In addition, muscle fiber-type shifts from slow to fast-twitch fibers. Unloading also results in a downregulation of heat shock proteins (e.g., HSP70) and anabolic signaling, which further exacerbate these morphological changes. Our lab recently showed reactive oxygen species (ROS) are causal in unloading-induced alterations in Akt and FoxO3a phosphorylation, muscle fiber atrophy, and fiber-type shift. Nutritional supplements such as fish oil and curcumin enhance anabolic signaling, glutathione levels, and heat shock proteins. We hypothesized that fish oil, rich in omega-3-fatty acids, combined with the polyphenol curcumin would enhance stress protective proteins and anabolic signaling in the rat soleus muscle, concomitant with synergistic protection of morphology. C57BL/6 mice were assigned to 3 groups (n = 6/group): ambulatory controls (CON), hindlimb unloading (HU), and hindlimb unloading with 5% fish oil, 1% curcumin in diet (FOC). FOC treatments began 10 days prior to HU and tissues were harvested following 7 days of HU. FOC mitigated the unloading induced decrease in CSA. FOC also enhanced abundance of HSP70 and anabolic signaling (Akt phosphorylation, p70S6K phosphorylation), while reducing Nox2, a source of oxidative stress. Therefore, we concluded that the combination of fish oil and curcumin prevents skeletal muscle atrophy due to a boost of heat shock proteins and anabolic signaling in an unloaded state. |
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Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloadingAnabolicAtrophyHSP70Nox2NutraceuticalSkeletal MuscleSpaceflightSkeletal muscle is a highly adaptable tissue capable of remodeling when dynamic stress is altered, including changes in mechanical loading and stretch. When muscle is subjected to an unloaded state (e.g., bedrest, immobilization, spaceflight) the resulting loss of muscle cross sectional area (CSA) impairs force production. In addition, muscle fiber-type shifts from slow to fast-twitch fibers. Unloading also results in a downregulation of heat shock proteins (e.g., HSP70) and anabolic signaling, which further exacerbate these morphological changes. Our lab recently showed reactive oxygen species (ROS) are causal in unloading-induced alterations in Akt and FoxO3a phosphorylation, muscle fiber atrophy, and fiber-type shift. Nutritional supplements such as fish oil and curcumin enhance anabolic signaling, glutathione levels, and heat shock proteins. We hypothesized that fish oil, rich in omega-3-fatty acids, combined with the polyphenol curcumin would enhance stress protective proteins and anabolic signaling in the rat soleus muscle, concomitant with synergistic protection of morphology. C57BL/6 mice were assigned to 3 groups (n = 6/group): ambulatory controls (CON), hindlimb unloading (HU), and hindlimb unloading with 5% fish oil, 1% curcumin in diet (FOC). FOC treatments began 10 days prior to HU and tissues were harvested following 7 days of HU. FOC mitigated the unloading induced decrease in CSA. FOC also enhanced abundance of HSP70 and anabolic signaling (Akt phosphorylation, p70S6K phosphorylation), while reducing Nox2, a source of oxidative stress. Therefore, we concluded that the combination of fish oil and curcumin prevents skeletal muscle atrophy due to a boost of heat shock proteins and anabolic signaling in an unloaded state.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Center for Research ResourcesNorges IdrettshøgskoleNational Aeronautics and Space AdministrationNational Space Biomedical Research InstituteRedox Biology & Cell Signaling Laboratory Department of Health and Kinesiology Texas A&M UniversityDepartment of Nutrition and Food Science Texas A&M UniversityDepartment of Cellular and Molecular Biology Federal University of ParaíbaDepartment of Molecular Physiology and Biophysics Carver School of Medicine University of IowaDepartment of Biomedical Engineering Georgia Tech UniversityDepartment of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)Muscle Biology Laboratory Department of Health and Kinesiology Texas A&M UniversityDepartment of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)National Center for Research Resources: 1 S10 RR22532-01Norges Idrettshøgskole: AR054084National Aeronautics and Space Administration: NNX13AE45GTexas A&M UniversityFederal University of ParaíbaUniversity of IowaGeorgia Tech UniversityUniversidade Estadual Paulista (Unesp)Lawler, John M.Garcia-Villatoro, Erika L.Guzzoni, ViniciusHord, Jeff M.Botchlett, RachelHolly, DylanLawler, Matthew S.Janini Gomes, Mariana [UNESP]Ryan, PatRodriguez, DinahKuczmarski, J. MatthewFluckey, James D.Talcott, Susanne2019-10-06T16:23:24Z2019-10-06T16:23:24Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-28http://dx.doi.org/10.1016/j.nutres.2018.12.013Nutrition Research, v. 65, p. 17-28.1879-07390271-5317http://hdl.handle.net/11449/18892110.1016/j.nutres.2018.12.0132-s2.0-85063724504Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNutrition Researchinfo:eu-repo/semantics/openAccess2024-08-14T17:22:59Zoai:repositorio.unesp.br:11449/188921Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
title |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
spellingShingle |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading Lawler, John M. Anabolic Atrophy HSP70 Nox2 Nutraceutical Skeletal Muscle Spaceflight |
title_short |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
title_full |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
title_fullStr |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
title_full_unstemmed |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
title_sort |
Effect of combined fish oil & Curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading |
author |
Lawler, John M. |
author_facet |
Lawler, John M. Garcia-Villatoro, Erika L. Guzzoni, Vinicius Hord, Jeff M. Botchlett, Rachel Holly, Dylan Lawler, Matthew S. Janini Gomes, Mariana [UNESP] Ryan, Pat Rodriguez, Dinah Kuczmarski, J. Matthew Fluckey, James D. Talcott, Susanne |
author_role |
author |
author2 |
Garcia-Villatoro, Erika L. Guzzoni, Vinicius Hord, Jeff M. Botchlett, Rachel Holly, Dylan Lawler, Matthew S. Janini Gomes, Mariana [UNESP] Ryan, Pat Rodriguez, Dinah Kuczmarski, J. Matthew Fluckey, James D. Talcott, Susanne |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Texas A&M University Federal University of Paraíba University of Iowa Georgia Tech University Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lawler, John M. Garcia-Villatoro, Erika L. Guzzoni, Vinicius Hord, Jeff M. Botchlett, Rachel Holly, Dylan Lawler, Matthew S. Janini Gomes, Mariana [UNESP] Ryan, Pat Rodriguez, Dinah Kuczmarski, J. Matthew Fluckey, James D. Talcott, Susanne |
dc.subject.por.fl_str_mv |
Anabolic Atrophy HSP70 Nox2 Nutraceutical Skeletal Muscle Spaceflight |
topic |
Anabolic Atrophy HSP70 Nox2 Nutraceutical Skeletal Muscle Spaceflight |
description |
Skeletal muscle is a highly adaptable tissue capable of remodeling when dynamic stress is altered, including changes in mechanical loading and stretch. When muscle is subjected to an unloaded state (e.g., bedrest, immobilization, spaceflight) the resulting loss of muscle cross sectional area (CSA) impairs force production. In addition, muscle fiber-type shifts from slow to fast-twitch fibers. Unloading also results in a downregulation of heat shock proteins (e.g., HSP70) and anabolic signaling, which further exacerbate these morphological changes. Our lab recently showed reactive oxygen species (ROS) are causal in unloading-induced alterations in Akt and FoxO3a phosphorylation, muscle fiber atrophy, and fiber-type shift. Nutritional supplements such as fish oil and curcumin enhance anabolic signaling, glutathione levels, and heat shock proteins. We hypothesized that fish oil, rich in omega-3-fatty acids, combined with the polyphenol curcumin would enhance stress protective proteins and anabolic signaling in the rat soleus muscle, concomitant with synergistic protection of morphology. C57BL/6 mice were assigned to 3 groups (n = 6/group): ambulatory controls (CON), hindlimb unloading (HU), and hindlimb unloading with 5% fish oil, 1% curcumin in diet (FOC). FOC treatments began 10 days prior to HU and tissues were harvested following 7 days of HU. FOC mitigated the unloading induced decrease in CSA. FOC also enhanced abundance of HSP70 and anabolic signaling (Akt phosphorylation, p70S6K phosphorylation), while reducing Nox2, a source of oxidative stress. Therefore, we concluded that the combination of fish oil and curcumin prevents skeletal muscle atrophy due to a boost of heat shock proteins and anabolic signaling in an unloaded state. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:23:24Z 2019-10-06T16:23:24Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.nutres.2018.12.013 Nutrition Research, v. 65, p. 17-28. 1879-0739 0271-5317 http://hdl.handle.net/11449/188921 10.1016/j.nutres.2018.12.013 2-s2.0-85063724504 |
url |
http://dx.doi.org/10.1016/j.nutres.2018.12.013 http://hdl.handle.net/11449/188921 |
identifier_str_mv |
Nutrition Research, v. 65, p. 17-28. 1879-0739 0271-5317 10.1016/j.nutres.2018.12.013 2-s2.0-85063724504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nutrition Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-28 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128142444855296 |