Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0007114520000963 http://hdl.handle.net/11449/201657 |
Resumo: | Less invasive protocols are necessary to study energy expenditure (EE) of cats living in homes for expressing their normal living conditions. The present study compared sampling times and the use of saliva, urine and blood to measure 2H and 18O to apply the doubly labelled water method. In the first study, four cats were used to evaluate the enrichment (2, 4, 6, 7 and 8 h) and elimination (2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 d) of 2H and 18O (subcutaneously injected). The maximum enrichment was after 5 h (R2 0·82) of injection, with an Ln linear elimination of both isotopes (P < 0·001; R2 0·99). The results of EE were similar, regardless of the sampling time used (P = 0·999). In the second study, seven male cats and seven female cats were used. Before and after isotope injection (5 h, 7 d, 10 d and 14 d), blood, saliva and urine were collected. Isotope enrichment was lower in urine (P < 0·05) and at the similar level in blood and saliva. Isotope elimination was similar for all fluids (P < 0·473). The EE calculated with blood and saliva was similar but higher for urine (P = 0·015). According to Bland-Altman statistics, blood and saliva presented low bias and high correlation (P < 0·001), but this was not observed for urine (P = 0·096). Higher EE was observed for male cats (384 (se 39) kJ/kg0·67 per d) than for female cats (337 (se 34) kJ/kg0·67 per d; P < 0·05). The sampling time for the method is flexible, and saliva can be used as a substitute for blood. |
id |
UNSP_2e89f8305a528776be14c3ed7984482c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/201657 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats2HEnergy metabolismFatty massFelinesLess invasive protocols are necessary to study energy expenditure (EE) of cats living in homes for expressing their normal living conditions. The present study compared sampling times and the use of saliva, urine and blood to measure 2H and 18O to apply the doubly labelled water method. In the first study, four cats were used to evaluate the enrichment (2, 4, 6, 7 and 8 h) and elimination (2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 d) of 2H and 18O (subcutaneously injected). The maximum enrichment was after 5 h (R2 0·82) of injection, with an Ln linear elimination of both isotopes (P < 0·001; R2 0·99). The results of EE were similar, regardless of the sampling time used (P = 0·999). In the second study, seven male cats and seven female cats were used. Before and after isotope injection (5 h, 7 d, 10 d and 14 d), blood, saliva and urine were collected. Isotope enrichment was lower in urine (P < 0·05) and at the similar level in blood and saliva. Isotope elimination was similar for all fluids (P < 0·473). The EE calculated with blood and saliva was similar but higher for urine (P = 0·015). According to Bland-Altman statistics, blood and saliva presented low bias and high correlation (P < 0·001), but this was not observed for urine (P = 0·096). Higher EE was observed for male cats (384 (se 39) kJ/kg0·67 per d) than for female cats (337 (se 34) kJ/kg0·67 per d; P < 0·05). The sampling time for the method is flexible, and saliva can be used as a substitute for blood.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp)Departamento de Zootecnia Universidade Federal da ParaíbaEscola de Medicina de Ribeirão Preto Universidade de São PauloFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp)Universidade Estadual Paulista (Unesp)Universidade Federal da Paraíba (UFPB)Universidade de São Paulo (USP)Goloni, Camila [UNESP]Peres, Francine M. [UNESP]Senhorello, Igor L. S. [UNESP]Di Santo, Ludmilla G. [UNESP]Mendonça, Fernanda S. [UNESP]Loureiro, Bruna A.Pfrimer, KarinaFerriolli, EduardoPereira, Gener T. [UNESP]Carciofi, Aulus C. [UNESP]2020-12-12T02:38:20Z2020-12-12T02:38:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article457-469http://dx.doi.org/10.1017/S0007114520000963British Journal of Nutrition, v. 124, n. 4, p. 457-469, 2020.1475-26620007-1145http://hdl.handle.net/11449/20165710.1017/S00071145200009632-s2.0-85082532395Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Nutritioninfo:eu-repo/semantics/openAccess2024-06-06T14:10:22Zoai:repositorio.unesp.br:11449/201657Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:54:36.742911Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
title |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
spellingShingle |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats Goloni, Camila [UNESP] 2H Energy metabolism Fatty mass Felines |
title_short |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
title_full |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
title_fullStr |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
title_full_unstemmed |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
title_sort |
Validation of saliva and urine use and sampling time on the doubly labelled water method to measure energy expenditure, body composition and water turnover in male and female cats |
author |
Goloni, Camila [UNESP] |
author_facet |
Goloni, Camila [UNESP] Peres, Francine M. [UNESP] Senhorello, Igor L. S. [UNESP] Di Santo, Ludmilla G. [UNESP] Mendonça, Fernanda S. [UNESP] Loureiro, Bruna A. Pfrimer, Karina Ferriolli, Eduardo Pereira, Gener T. [UNESP] Carciofi, Aulus C. [UNESP] |
author_role |
author |
author2 |
Peres, Francine M. [UNESP] Senhorello, Igor L. S. [UNESP] Di Santo, Ludmilla G. [UNESP] Mendonça, Fernanda S. [UNESP] Loureiro, Bruna A. Pfrimer, Karina Ferriolli, Eduardo Pereira, Gener T. [UNESP] Carciofi, Aulus C. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal da Paraíba (UFPB) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Goloni, Camila [UNESP] Peres, Francine M. [UNESP] Senhorello, Igor L. S. [UNESP] Di Santo, Ludmilla G. [UNESP] Mendonça, Fernanda S. [UNESP] Loureiro, Bruna A. Pfrimer, Karina Ferriolli, Eduardo Pereira, Gener T. [UNESP] Carciofi, Aulus C. [UNESP] |
dc.subject.por.fl_str_mv |
2H Energy metabolism Fatty mass Felines |
topic |
2H Energy metabolism Fatty mass Felines |
description |
Less invasive protocols are necessary to study energy expenditure (EE) of cats living in homes for expressing their normal living conditions. The present study compared sampling times and the use of saliva, urine and blood to measure 2H and 18O to apply the doubly labelled water method. In the first study, four cats were used to evaluate the enrichment (2, 4, 6, 7 and 8 h) and elimination (2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 d) of 2H and 18O (subcutaneously injected). The maximum enrichment was after 5 h (R2 0·82) of injection, with an Ln linear elimination of both isotopes (P < 0·001; R2 0·99). The results of EE were similar, regardless of the sampling time used (P = 0·999). In the second study, seven male cats and seven female cats were used. Before and after isotope injection (5 h, 7 d, 10 d and 14 d), blood, saliva and urine were collected. Isotope enrichment was lower in urine (P < 0·05) and at the similar level in blood and saliva. Isotope elimination was similar for all fluids (P < 0·473). The EE calculated with blood and saliva was similar but higher for urine (P = 0·015). According to Bland-Altman statistics, blood and saliva presented low bias and high correlation (P < 0·001), but this was not observed for urine (P = 0·096). Higher EE was observed for male cats (384 (se 39) kJ/kg0·67 per d) than for female cats (337 (se 34) kJ/kg0·67 per d; P < 0·05). The sampling time for the method is flexible, and saliva can be used as a substitute for blood. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:38:20Z 2020-12-12T02:38:20Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0007114520000963 British Journal of Nutrition, v. 124, n. 4, p. 457-469, 2020. 1475-2662 0007-1145 http://hdl.handle.net/11449/201657 10.1017/S0007114520000963 2-s2.0-85082532395 |
url |
http://dx.doi.org/10.1017/S0007114520000963 http://hdl.handle.net/11449/201657 |
identifier_str_mv |
British Journal of Nutrition, v. 124, n. 4, p. 457-469, 2020. 1475-2662 0007-1145 10.1017/S0007114520000963 2-s2.0-85082532395 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
British Journal of Nutrition |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
457-469 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129138433720320 |