The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2174/1871530319666191204130007 http://hdl.handle.net/11449/198938 |
Resumo: | Background: Limited studies have been carried out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of β-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM+PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and immunoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high-density and very lowdensity lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should be replaced in order to not to aggravate this condition. |
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The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell responseDiabetesGlucocorticoidsGlycometabolismImmunosuppressantLipometabolismPrednisoneBackground: Limited studies have been carried out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of β-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM+PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and immunoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high-density and very lowdensity lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should be replaced in order to not to aggravate this condition.Postgraduate Program in Pharmacology and Biotechnology São Paulo State University (UNESP) Institute of BiosciencesDepartment of Physiology Faculty of Medicine of Ribeirão Preto University of São PauloLaboratory of System Physiology and Reproductive Toxicology Institute of Biological Sciences and Health Federal University of Mato Grosso (UFMT)Postgraduate Program in Pharmacology and Biotechnology São Paulo State University (UNESP) Institute of BiosciencesUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Federal University of Mato Grosso (UFMT)Machado, Mariana P. R. [UNESP]Schavinski, Aline Z.Deluque, Amanda L.Volpato, Gustavo T.Campos, Kleber E.2020-12-12T01:26:01Z2020-12-12T01:26:01Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article797-805http://dx.doi.org/10.2174/1871530319666191204130007Endocrine, Metabolic and Immune Disorders - Drug Targets, v. 20, n. 5, p. 797-805, 2020.2212-38731871-5303http://hdl.handle.net/11449/19893810.2174/18715303196661912041300072-s2.0-85085961513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEndocrine, Metabolic and Immune Disorders - Drug Targetsinfo:eu-repo/semantics/openAccess2021-10-22T21:09:40Zoai:repositorio.unesp.br:11449/198938Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:34:12.802501Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
title |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
spellingShingle |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response Machado, Mariana P. R. [UNESP] Diabetes Glucocorticoids Glycometabolism Immunosuppressant Lipometabolism Prednisone |
title_short |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
title_full |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
title_fullStr |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
title_full_unstemmed |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
title_sort |
The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response |
author |
Machado, Mariana P. R. [UNESP] |
author_facet |
Machado, Mariana P. R. [UNESP] Schavinski, Aline Z. Deluque, Amanda L. Volpato, Gustavo T. Campos, Kleber E. |
author_role |
author |
author2 |
Schavinski, Aline Z. Deluque, Amanda L. Volpato, Gustavo T. Campos, Kleber E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Federal University of Mato Grosso (UFMT) |
dc.contributor.author.fl_str_mv |
Machado, Mariana P. R. [UNESP] Schavinski, Aline Z. Deluque, Amanda L. Volpato, Gustavo T. Campos, Kleber E. |
dc.subject.por.fl_str_mv |
Diabetes Glucocorticoids Glycometabolism Immunosuppressant Lipometabolism Prednisone |
topic |
Diabetes Glucocorticoids Glycometabolism Immunosuppressant Lipometabolism Prednisone |
description |
Background: Limited studies have been carried out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of β-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM+PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and immunoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high-density and very lowdensity lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should be replaced in order to not to aggravate this condition. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:26:01Z 2020-12-12T01:26:01Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2174/1871530319666191204130007 Endocrine, Metabolic and Immune Disorders - Drug Targets, v. 20, n. 5, p. 797-805, 2020. 2212-3873 1871-5303 http://hdl.handle.net/11449/198938 10.2174/1871530319666191204130007 2-s2.0-85085961513 |
url |
http://dx.doi.org/10.2174/1871530319666191204130007 http://hdl.handle.net/11449/198938 |
identifier_str_mv |
Endocrine, Metabolic and Immune Disorders - Drug Targets, v. 20, n. 5, p. 797-805, 2020. 2212-3873 1871-5303 10.2174/1871530319666191204130007 2-s2.0-85085961513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Endocrine, Metabolic and Immune Disorders - Drug Targets |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
797-805 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129088069566464 |