Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells

• Autor(a) principal: Santiago, Karina Basso [UNESP]
• Data de Publicação: 2023
• Outros Autores: Conti, Bruno Jose [UNESP], Cardoso, Eliza de Oliveira [UNESP], Conte, Fernanda Lopes [UNESP], Tasca, Karen Ingrid [UNESP], Romagnoli, Graziela Gorete [UNESP], Golim, Marjorie de Assis [UNESP], Cruz, Maria Tereza, Sforcin, Jose Mauricio [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0044; http://hdl.handle.net/11449/245797
• topic: Dendritic cells; T CD4+cells; MAGE-1; Retinoic acid; Propolis; Immunomodulation

Background: Propolis exhibits huge potential in the pharmaceutical industry. In the present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) and on T lymphocytes to observe a possible differential activation of T lymphocytes, driving preferentially to Th1 or Treg cells.Methods: Cell viability, lymphocyte proliferation, gene expression (T-bet and FoxP3), and cytokine production by DCs (TNF-alpha, IL-10, IL-6 and IL-1 beta) and lymphocytes (IFN-gamma and TGF-beta) were analyzed.Results: MAGE-1 and RA alone or in combination with propolis inhibited TNF-alpha production and induced a higher lymphoproliferation compared to control, while MAGE-1 + propolis induced IL-6 production. Propolis in combination with RA induced FoxP3 expression. MAGE-1 induced IFN-gamma production while propolis inhibited it, returning to basal levels. RA inhibited TGF-beta production, what was counteracted by propolis.Conclusion: Propolis affected immunological parameters inhibiting pro-inflammatory cytokines and favoring the regulatory profile, opening perspectives for the control of inflammatory conditions.