The relevance of miRNAs as promising biomarkers in lip cancer
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-020-03773-9 http://hdl.handle.net/11449/209903 |
Resumo: | Objectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression. |
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The relevance of miRNAs as promising biomarkers in lip cancerOral cancermiRLip cancerActinic cheilitisObjectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Bauru Sch Dent, Area Pathol, Dept Surg Stomatol Pathol & Radiol, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Histopathol, Sao Paulo, BrazilAC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Histopathol, Sao Paulo, BrazilCNPq: 870891/1999-5CAPES: 001SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)AC Camargo Canc CtrAssao, AgnesDomingues, Maria Aparecida Custodio [UNESP]Minicucci, Eliana Maria [UNESP]Marchi, Fabio AlbuquerqueCoutinho-Camillo, Claudia MalheirosOliveira, Denise Tostes2021-06-25T12:33:10Z2021-06-25T12:33:10Z2021-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1007/s00784-020-03773-9Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021.1432-6981http://hdl.handle.net/11449/20990310.1007/s00784-020-03773-9WOS:000607507200001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-03T13:14:14Zoai:repositorio.unesp.br:11449/209903Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The relevance of miRNAs as promising biomarkers in lip cancer |
title |
The relevance of miRNAs as promising biomarkers in lip cancer |
spellingShingle |
The relevance of miRNAs as promising biomarkers in lip cancer Assao, Agnes Oral cancer miR Lip cancer Actinic cheilitis |
title_short |
The relevance of miRNAs as promising biomarkers in lip cancer |
title_full |
The relevance of miRNAs as promising biomarkers in lip cancer |
title_fullStr |
The relevance of miRNAs as promising biomarkers in lip cancer |
title_full_unstemmed |
The relevance of miRNAs as promising biomarkers in lip cancer |
title_sort |
The relevance of miRNAs as promising biomarkers in lip cancer |
author |
Assao, Agnes |
author_facet |
Assao, Agnes Domingues, Maria Aparecida Custodio [UNESP] Minicucci, Eliana Maria [UNESP] Marchi, Fabio Albuquerque Coutinho-Camillo, Claudia Malheiros Oliveira, Denise Tostes |
author_role |
author |
author2 |
Domingues, Maria Aparecida Custodio [UNESP] Minicucci, Eliana Maria [UNESP] Marchi, Fabio Albuquerque Coutinho-Camillo, Claudia Malheiros Oliveira, Denise Tostes |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) AC Camargo Canc Ctr |
dc.contributor.author.fl_str_mv |
Assao, Agnes Domingues, Maria Aparecida Custodio [UNESP] Minicucci, Eliana Maria [UNESP] Marchi, Fabio Albuquerque Coutinho-Camillo, Claudia Malheiros Oliveira, Denise Tostes |
dc.subject.por.fl_str_mv |
Oral cancer miR Lip cancer Actinic cheilitis |
topic |
Oral cancer miR Lip cancer Actinic cheilitis |
description |
Objectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:33:10Z 2021-06-25T12:33:10Z 2021-01-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-020-03773-9 Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021. 1432-6981 http://hdl.handle.net/11449/209903 10.1007/s00784-020-03773-9 WOS:000607507200001 |
url |
http://dx.doi.org/10.1007/s00784-020-03773-9 http://hdl.handle.net/11449/209903 |
identifier_str_mv |
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021. 1432-6981 10.1007/s00784-020-03773-9 WOS:000607507200001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021358394933248 |