The relevance of miRNAs as promising biomarkers in lip cancer

Detalhes bibliográficos
Autor(a) principal: Assao, Agnes
Data de Publicação: 2021
Outros Autores: Domingues, Maria Aparecida Custodio [UNESP], Minicucci, Eliana Maria [UNESP], Marchi, Fabio Albuquerque, Coutinho-Camillo, Claudia Malheiros, Oliveira, Denise Tostes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-020-03773-9
http://hdl.handle.net/11449/209903
Resumo: Objectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
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spelling The relevance of miRNAs as promising biomarkers in lip cancerOral cancermiRLip cancerActinic cheilitisObjectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Bauru Sch Dent, Area Pathol, Dept Surg Stomatol Pathol & Radiol, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Histopathol, Sao Paulo, BrazilAC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Histopathol, Sao Paulo, BrazilCNPq: 870891/1999-5CAPES: 001SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)AC Camargo Canc CtrAssao, AgnesDomingues, Maria Aparecida Custodio [UNESP]Minicucci, Eliana Maria [UNESP]Marchi, Fabio AlbuquerqueCoutinho-Camillo, Claudia MalheirosOliveira, Denise Tostes2021-06-25T12:33:10Z2021-06-25T12:33:10Z2021-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1007/s00784-020-03773-9Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021.1432-6981http://hdl.handle.net/11449/20990310.1007/s00784-020-03773-9WOS:000607507200001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-03T13:14:14Zoai:repositorio.unesp.br:11449/209903Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The relevance of miRNAs as promising biomarkers in lip cancer
title The relevance of miRNAs as promising biomarkers in lip cancer
spellingShingle The relevance of miRNAs as promising biomarkers in lip cancer
Assao, Agnes
Oral cancer
miR
Lip cancer
Actinic cheilitis
title_short The relevance of miRNAs as promising biomarkers in lip cancer
title_full The relevance of miRNAs as promising biomarkers in lip cancer
title_fullStr The relevance of miRNAs as promising biomarkers in lip cancer
title_full_unstemmed The relevance of miRNAs as promising biomarkers in lip cancer
title_sort The relevance of miRNAs as promising biomarkers in lip cancer
author Assao, Agnes
author_facet Assao, Agnes
Domingues, Maria Aparecida Custodio [UNESP]
Minicucci, Eliana Maria [UNESP]
Marchi, Fabio Albuquerque
Coutinho-Camillo, Claudia Malheiros
Oliveira, Denise Tostes
author_role author
author2 Domingues, Maria Aparecida Custodio [UNESP]
Minicucci, Eliana Maria [UNESP]
Marchi, Fabio Albuquerque
Coutinho-Camillo, Claudia Malheiros
Oliveira, Denise Tostes
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
AC Camargo Canc Ctr
dc.contributor.author.fl_str_mv Assao, Agnes
Domingues, Maria Aparecida Custodio [UNESP]
Minicucci, Eliana Maria [UNESP]
Marchi, Fabio Albuquerque
Coutinho-Camillo, Claudia Malheiros
Oliveira, Denise Tostes
dc.subject.por.fl_str_mv Oral cancer
miR
Lip cancer
Actinic cheilitis
topic Oral cancer
miR
Lip cancer
Actinic cheilitis
description Objectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation. Materials and methods The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square. Results A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers. Conclusions The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:33:10Z
2021-06-25T12:33:10Z
2021-01-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-020-03773-9
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021.
1432-6981
http://hdl.handle.net/11449/209903
10.1007/s00784-020-03773-9
WOS:000607507200001
url http://dx.doi.org/10.1007/s00784-020-03773-9
http://hdl.handle.net/11449/209903
identifier_str_mv Clinical Oral Investigations. Heidelberg: Springer Heidelberg, 8 p., 2021.
1432-6981
10.1007/s00784-020-03773-9
WOS:000607507200001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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