Toxicidade celular e ação do EGCG sobre biofilme simples e misto de microrganismos de interesse endodôntico

• Autor(a) principal: Assumpção, Thainara de Oliveira [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/203994; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-04-18/000913818.pdf
• topic: Flavonoides; Biofilmes; Anti-infecciosos; Flavonoids

One of the main causes of failure in endodontic therapy is the persistence of microbial infection even after chemical-mechanical preparation and application of intracanal medication. Many authors have explored the use of herbal medicines in order to obtain new compounds that have therapeutic properties without causing microbial resistance. Among these are the flavonoids, natural phenolic compounds which have different properties such as antimicrobial, antioxidant, osteogenic and antiosteoclastogenic. One of these flavonoid that has received attention in the literature is epigallocatechin-3-gallate (EGCG), which has also shown effect against oral pathogens. Thus, the objectives of this study were to evaluate the antimicrobial activity of EGCG on planktonic culture and simple or dual-species biofilms of microorganisms resistant to endodontic treatment, as well as evaluate its toxicity on culture of fibroblasts. Tests were performed in the planktonic culture to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EGCG against Enterococcus faecalis, Streptococcus mutans, Actinomyces israelii, Pseudomonas aeruginosa and Fusobacterium nucleatum and also biofilm assays with some of these microorganisms in combination with E. faecalis. In addition, the cytotoxicity of EGCG was determined on L-929 fibroblast line after 24 h exposure using the MTT assay. Data were statistically analyzed using ANOVA/Tukey tests, considering p<0.05. The results showed that EGCG showed a bactericidal effect against all the evaluated species, with values of CBM varying between 0.03 and 2 mg/mL. except by P. aeruginosa. The elimination of the viable cells from the single biofilm of S. mutans and F. nucleatum and reduction of the biofilms of E. faecalis and A. israelii were observed after treatment of the biofilms with EGCG. It has also...