Atividade antimicrobiana e antibiofilme de flavonois como agentes para aplicação endodôntica

• Autor(a) principal: Rabelo, Rafaela Laruzo [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/203978; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-04-18/000913672.pdf
• topic: Flavonoides; Anti-infecciosos; Biofilmes; Flavonoids

Even after endodontic treatment, in permanent teeth with open apex or closed, refractory infections may develop due to the persistence or invasion of new pathogenic microorganisms. Many authors have explored the use of phytotherapics in order to obtain new compounds that have th erapeutic properties, such as flavonoids. An important class of flavonoids is the flavonois, characterized by a chemical skeleton of 3-hydroxyflavone, which may or may not be glycosylated (or bound to a sugar). They are present in fruits and vegetables and have several properties, such as antimicrobial, antioxidant, osteogenic and antiosteoclastogenic properties. Therefore, the objective of this study is to analyze the antimicrobial and antibiofilm activity of flavonols as agents for endodontic application. The following flavonols: myricetin, kaempferol, quercetin and galangin were evaluated against Streptococcus mutans, Enterococcus faecalis, Lactobacillus casei, Actinomyces israelii, Fusobacterium nucleatum and Candida albicans in planktonic culture and 48 hours biofilm in polystyrene plates, after 24 hours of treament. The results showed MIC/MBC values ranging from 0.03 to 2 mg/mL. Kaempferol and quercetin had no effect against any tested microorganism. Galangin reduced the growth of C. albicans and A. israelii, and myricetin had bactericidal action against S. mutans, A. israelii, L. casei and F. nucleatum. For the antibiofilm action, galangin significantly reduced biofilm of C. albicans and A. israelii. Mirycetin eliminated S. mutans and E. faecalis biofilms and reduced A. israelii, L. casei and F. nucleatum biofilms. It is concluded that, among the flavonols tested, myricetin had the best antimicrobial and antibiotic effects against microorganisms associated with endodontic infection