Expression of glycosylated proteins in bacterial system and purification by affinity chromatography

Detalhes bibliográficos
Autor(a) principal: Breyer, Carlos Alexandre [UNESP]
Data de Publicação: 2018
Outros Autores: de Oliveira, Marcos Antonio [UNESP], Pessoa, Adalberto
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-4939-7312-5_14
http://hdl.handle.net/11449/228390
Resumo: The bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for the purification of glycosylated proteins to attain homogeneity. In this chapter we present a general process for glycoprotein production in Escherichia coli and a practice method for purification of glycosylated proteins, using affinity chromatography.
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spelling Expression of glycosylated proteins in bacterial system and purification by affinity chromatographyAffinity chromatographyBacterial expressionConjugate vaccinesGlycosylated proteinThe bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for the purification of glycosylated proteins to attain homogeneity. In this chapter we present a general process for glycoprotein production in Escherichia coli and a practice method for purification of glycosylated proteins, using affinity chromatography.Biosciences Institute São Paulo State University, Coastal Campus, São VicenteSchool of Pharmaceutical Sciences University of São PauloInstituto de Biociências Universidade Estadual PaulistaBiosciences Institute São Paulo State University, Coastal Campus, São VicenteInstituto de Biociências Universidade Estadual PaulistaUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Breyer, Carlos Alexandre [UNESP]de Oliveira, Marcos Antonio [UNESP]Pessoa, Adalberto2022-04-29T08:14:25Z2022-04-29T08:14:25Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart183-191http://dx.doi.org/10.1007/978-1-4939-7312-5_14Methods in Molecular Biology, v. 1674, p. 183-191.1064-3745http://hdl.handle.net/11449/22839010.1007/978-1-4939-7312-5_142-s2.0-85029700402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:14:25Zoai:repositorio.unesp.br:11449/228390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:58:30.906953Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
title Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
spellingShingle Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
Breyer, Carlos Alexandre [UNESP]
Affinity chromatography
Bacterial expression
Conjugate vaccines
Glycosylated protein
title_short Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
title_full Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
title_fullStr Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
title_full_unstemmed Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
title_sort Expression of glycosylated proteins in bacterial system and purification by affinity chromatography
author Breyer, Carlos Alexandre [UNESP]
author_facet Breyer, Carlos Alexandre [UNESP]
de Oliveira, Marcos Antonio [UNESP]
Pessoa, Adalberto
author_role author
author2 de Oliveira, Marcos Antonio [UNESP]
Pessoa, Adalberto
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Breyer, Carlos Alexandre [UNESP]
de Oliveira, Marcos Antonio [UNESP]
Pessoa, Adalberto
dc.subject.por.fl_str_mv Affinity chromatography
Bacterial expression
Conjugate vaccines
Glycosylated protein
topic Affinity chromatography
Bacterial expression
Conjugate vaccines
Glycosylated protein
description The bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for the purification of glycosylated proteins to attain homogeneity. In this chapter we present a general process for glycoprotein production in Escherichia coli and a practice method for purification of glycosylated proteins, using affinity chromatography.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
2022-04-29T08:14:25Z
2022-04-29T08:14:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-4939-7312-5_14
Methods in Molecular Biology, v. 1674, p. 183-191.
1064-3745
http://hdl.handle.net/11449/228390
10.1007/978-1-4939-7312-5_14
2-s2.0-85029700402
url http://dx.doi.org/10.1007/978-1-4939-7312-5_14
http://hdl.handle.net/11449/228390
identifier_str_mv Methods in Molecular Biology, v. 1674, p. 183-191.
1064-3745
10.1007/978-1-4939-7312-5_14
2-s2.0-85029700402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 183-191
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129144817451008

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