Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.21475/ajcs.17.11.04.384 http://hdl.handle.net/11449/228310 |
Resumo: | The aim of this investigation was to evaluate cryopreservation by vitrification of seeds from the orchid species Cattleya walkeriana and to achieve ex vitro acclimatization of in vitro derived cryopreserved seedlings. Seeds were removed from eight-month-old and ten-month-old capsules, and immersed in LN under different PVS2 exposure times. In in vitro experiment, ten different treatments and five replications with approximately 600 seeds each one were distributed in a completely random design and germination was calculated by the number of germinated seeds developing into protocorms and subsequently into seedlings. Seeds that were immersed directly into LN (0 min PVS2) and germinated in 1/2 MS culture medium after plunged directly into LN, returned germination rates of 3.0 (eight-month-old) and 36.8% (ten-month-old). Ten-month-old seeds in vitrification for 1h in PVS2 returned 53% germination in 1/2 MS culture medium. However, when the same mature seeds were exposed for 1h to PVS2 with the addition of 1% phloroglucinol, germination was 4.36% higher compared to the treatment without this additive. Seedlings from cryopreserved seeds showed normal development after 210 days of in vitro culture. Seedlings from ten-month-old seeds cryopreserved for 1h in PVS2 were acclimatized in greenhouse conditions in either sphagnum moss or sphagnum moss with pine bark (1:1, v/v) revealed the best ex vitro growth. Seed cryopreservation of this orchid species has shown to be efficient, demonstrating the effect of seed maturation time and ex vitro seedling acclimatization was established, with successful development of seedlings. |
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Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchidAcclimatizationGermplasm conservationOrchidaceaePhloroglucinolVitrificationThe aim of this investigation was to evaluate cryopreservation by vitrification of seeds from the orchid species Cattleya walkeriana and to achieve ex vitro acclimatization of in vitro derived cryopreserved seedlings. Seeds were removed from eight-month-old and ten-month-old capsules, and immersed in LN under different PVS2 exposure times. In in vitro experiment, ten different treatments and five replications with approximately 600 seeds each one were distributed in a completely random design and germination was calculated by the number of germinated seeds developing into protocorms and subsequently into seedlings. Seeds that were immersed directly into LN (0 min PVS2) and germinated in 1/2 MS culture medium after plunged directly into LN, returned germination rates of 3.0 (eight-month-old) and 36.8% (ten-month-old). Ten-month-old seeds in vitrification for 1h in PVS2 returned 53% germination in 1/2 MS culture medium. However, when the same mature seeds were exposed for 1h to PVS2 with the addition of 1% phloroglucinol, germination was 4.36% higher compared to the treatment without this additive. Seedlings from cryopreserved seeds showed normal development after 210 days of in vitro culture. Seedlings from ten-month-old seeds cryopreserved for 1h in PVS2 were acclimatized in greenhouse conditions in either sphagnum moss or sphagnum moss with pine bark (1:1, v/v) revealed the best ex vitro growth. Seed cryopreservation of this orchid species has shown to be efficient, demonstrating the effect of seed maturation time and ex vitro seedling acclimatization was established, with successful development of seedlings.Department of Technology Univ Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nUniversity of Florida Tropical Research and Education Center, 18905 SW 280th StUniversidade Estadual de Londrina Centro de Ciências Agrárias, Caixa Postal 6001Department of Technology Univ Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/nUniversidade Estadual Paulista (UNESP)Tropical Research and Education CenterUniversidade Estadual de Londrina (UEL)Galdiano, Renato Fernandes [UNESP]Vendrame, Wagner AparecidoMoretto, Cristiane [UNESP]de Faria, Ricardo TadeuLemos, Eliana Gertrudes de Macedo [UNESP]2022-04-29T08:03:54Z2022-04-29T08:03:54Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article485-490http://dx.doi.org/10.21475/ajcs.17.11.04.384Australian Journal of Crop Science, v. 11, n. 4, p. 485-490, 2017.1835-27071835-2693http://hdl.handle.net/11449/22831010.21475/ajcs.17.11.04.3842-s2.0-85018353910Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAustralian Journal of Crop Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:03:54Zoai:repositorio.unesp.br:11449/228310Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:03:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
title |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
spellingShingle |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid Galdiano, Renato Fernandes [UNESP] Acclimatization Germplasm conservation Orchidaceae Phloroglucinol Vitrification |
title_short |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
title_full |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
title_fullStr |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
title_full_unstemmed |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
title_sort |
Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid |
author |
Galdiano, Renato Fernandes [UNESP] |
author_facet |
Galdiano, Renato Fernandes [UNESP] Vendrame, Wagner Aparecido Moretto, Cristiane [UNESP] de Faria, Ricardo Tadeu Lemos, Eliana Gertrudes de Macedo [UNESP] |
author_role |
author |
author2 |
Vendrame, Wagner Aparecido Moretto, Cristiane [UNESP] de Faria, Ricardo Tadeu Lemos, Eliana Gertrudes de Macedo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Tropical Research and Education Center Universidade Estadual de Londrina (UEL) |
dc.contributor.author.fl_str_mv |
Galdiano, Renato Fernandes [UNESP] Vendrame, Wagner Aparecido Moretto, Cristiane [UNESP] de Faria, Ricardo Tadeu Lemos, Eliana Gertrudes de Macedo [UNESP] |
dc.subject.por.fl_str_mv |
Acclimatization Germplasm conservation Orchidaceae Phloroglucinol Vitrification |
topic |
Acclimatization Germplasm conservation Orchidaceae Phloroglucinol Vitrification |
description |
The aim of this investigation was to evaluate cryopreservation by vitrification of seeds from the orchid species Cattleya walkeriana and to achieve ex vitro acclimatization of in vitro derived cryopreserved seedlings. Seeds were removed from eight-month-old and ten-month-old capsules, and immersed in LN under different PVS2 exposure times. In in vitro experiment, ten different treatments and five replications with approximately 600 seeds each one were distributed in a completely random design and germination was calculated by the number of germinated seeds developing into protocorms and subsequently into seedlings. Seeds that were immersed directly into LN (0 min PVS2) and germinated in 1/2 MS culture medium after plunged directly into LN, returned germination rates of 3.0 (eight-month-old) and 36.8% (ten-month-old). Ten-month-old seeds in vitrification for 1h in PVS2 returned 53% germination in 1/2 MS culture medium. However, when the same mature seeds were exposed for 1h to PVS2 with the addition of 1% phloroglucinol, germination was 4.36% higher compared to the treatment without this additive. Seedlings from cryopreserved seeds showed normal development after 210 days of in vitro culture. Seedlings from ten-month-old seeds cryopreserved for 1h in PVS2 were acclimatized in greenhouse conditions in either sphagnum moss or sphagnum moss with pine bark (1:1, v/v) revealed the best ex vitro growth. Seed cryopreservation of this orchid species has shown to be efficient, demonstrating the effect of seed maturation time and ex vitro seedling acclimatization was established, with successful development of seedlings. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2022-04-29T08:03:54Z 2022-04-29T08:03:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.21475/ajcs.17.11.04.384 Australian Journal of Crop Science, v. 11, n. 4, p. 485-490, 2017. 1835-2707 1835-2693 http://hdl.handle.net/11449/228310 10.21475/ajcs.17.11.04.384 2-s2.0-85018353910 |
url |
http://dx.doi.org/10.21475/ajcs.17.11.04.384 http://hdl.handle.net/11449/228310 |
identifier_str_mv |
Australian Journal of Crop Science, v. 11, n. 4, p. 485-490, 2017. 1835-2707 1835-2693 10.21475/ajcs.17.11.04.384 2-s2.0-85018353910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Australian Journal of Crop Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
485-490 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965589194670080 |