Use of weathered diesel oil as a low-cost raw material for biosurfactant production
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0104-66322008000200006 http://hdl.handle.net/11449/20111 |
Resumo: | This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the de Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures. |
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Repositório Institucional da UNESP |
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Use of weathered diesel oil as a low-cost raw material for biosurfactant productionBiosurfactantDiesel oilRaw materialWeatheredThis work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the de Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Instituto de Geociências e Ciências ExatasUniversidade Estadual Paulista Instituto de Biociências Departamento de Bioquímica e MicrobiologiaUniversidade Estadual Paulista Instituto de Geociências e Ciências ExatasUniversidade Estadual Paulista Instituto de Biociências Departamento de Bioquímica e MicrobiologiaBrazilian Society of Chemical EngineeringUniversidade Estadual Paulista (Unesp)Mariano, A. P. [UNESP]Bonotto, Daniel Marcos [UNESP]Angelis, D. F. [UNESP]Pirôllo, M. P. S. [UNESP]Contiero, Jonas [UNESP]2013-09-30T20:03:01Z2014-05-20T13:56:16Z2013-09-30T20:03:01Z2014-05-20T13:56:16Z2008-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article269-274application/pdfhttp://dx.doi.org/10.1590/S0104-66322008000200006Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 25, n. 2, p. 269-274, 2008.0104-6632http://hdl.handle.net/11449/2011110.1590/S0104-66322008000200006S0104-66322008000200006WOS:000256309800006S0104-66322008000200006.pdf7430102726026121SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Chemical Engineering0.9250,395info:eu-repo/semantics/openAccess2023-11-22T06:17:54Zoai:repositorio.unesp.br:11449/20111Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:26:58.352408Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
title |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
spellingShingle |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production Mariano, A. P. [UNESP] Biosurfactant Diesel oil Raw material Weathered |
title_short |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
title_full |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
title_fullStr |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
title_full_unstemmed |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
title_sort |
Use of weathered diesel oil as a low-cost raw material for biosurfactant production |
author |
Mariano, A. P. [UNESP] |
author_facet |
Mariano, A. P. [UNESP] Bonotto, Daniel Marcos [UNESP] Angelis, D. F. [UNESP] Pirôllo, M. P. S. [UNESP] Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Bonotto, Daniel Marcos [UNESP] Angelis, D. F. [UNESP] Pirôllo, M. P. S. [UNESP] Contiero, Jonas [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mariano, A. P. [UNESP] Bonotto, Daniel Marcos [UNESP] Angelis, D. F. [UNESP] Pirôllo, M. P. S. [UNESP] Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
Biosurfactant Diesel oil Raw material Weathered |
topic |
Biosurfactant Diesel oil Raw material Weathered |
description |
This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the de Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 2013-09-30T20:03:01Z 2013-09-30T20:03:01Z 2014-05-20T13:56:16Z 2014-05-20T13:56:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0104-66322008000200006 Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 25, n. 2, p. 269-274, 2008. 0104-6632 http://hdl.handle.net/11449/20111 10.1590/S0104-66322008000200006 S0104-66322008000200006 WOS:000256309800006 S0104-66322008000200006.pdf 7430102726026121 |
url |
http://dx.doi.org/10.1590/S0104-66322008000200006 http://hdl.handle.net/11449/20111 |
identifier_str_mv |
Brazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 25, n. 2, p. 269-274, 2008. 0104-6632 10.1590/S0104-66322008000200006 S0104-66322008000200006 WOS:000256309800006 S0104-66322008000200006.pdf 7430102726026121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Chemical Engineering 0.925 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
269-274 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128933873319936 |