RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbalip.2022.159276 http://hdl.handle.net/11449/249576 |
Resumo: | The anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies. |
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RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytesAdenosineATPHuman adipocytesIrisinObesityPurinergic signalingThyroid hormonesThe anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)European Social FundFundação para a Ciência e a TecnologiaDepartment of Internal Clinic Botucatu Medical School São Paulo State University (UNESP), São PauloLaboratório de Farmacologia e Neurobiologia Departamento de Imuno-Fisiologia e Farmacologia Instituto de Ciências Biomédicas de Abel Salazar Universidade do Porto (ICBAS-UP)Center for Drug Discovery and Innovative Medicines (MedInUP) ICBAS-UPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloDepartment of Internal Clinic Botucatu Medical School São Paulo State University (UNESP), São PauloDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloFAPESP: 2016/03242-3FAPESP: 2020/06763-0CNPq: 409438/2016European Social Fund: NORTE-08-5369-FSE-000011Fundação para a Ciência e a Tecnologia: UIDB/04308/2020Universidade Estadual Paulista (UNESP)Universidade do Porto (ICBAS-UP)ICBAS-UPMathias, Lucas Solla [UNESP]Herman-de-Sousa, CarinaCury, Sarah Santiloni [UNESP]Nogueira, Célia Regina [UNESP]Correia-de-Sá, Paulode Oliveira, Miriane [UNESP]2023-07-29T16:03:31Z2023-07-29T16:03:31Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbalip.2022.159276Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023.1879-26181388-1981http://hdl.handle.net/11449/24957610.1016/j.bbalip.2022.1592762-s2.0-85146576762Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipidsinfo:eu-repo/semantics/openAccess2024-08-14T17:22:48Zoai:repositorio.unesp.br:11449/249576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
title |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
spellingShingle |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes Mathias, Lucas Solla [UNESP] Adenosine ATP Human adipocytes Irisin Obesity Purinergic signaling Thyroid hormones |
title_short |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
title_full |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
title_fullStr |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
title_full_unstemmed |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
title_sort |
RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes |
author |
Mathias, Lucas Solla [UNESP] |
author_facet |
Mathias, Lucas Solla [UNESP] Herman-de-Sousa, Carina Cury, Sarah Santiloni [UNESP] Nogueira, Célia Regina [UNESP] Correia-de-Sá, Paulo de Oliveira, Miriane [UNESP] |
author_role |
author |
author2 |
Herman-de-Sousa, Carina Cury, Sarah Santiloni [UNESP] Nogueira, Célia Regina [UNESP] Correia-de-Sá, Paulo de Oliveira, Miriane [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade do Porto (ICBAS-UP) ICBAS-UP |
dc.contributor.author.fl_str_mv |
Mathias, Lucas Solla [UNESP] Herman-de-Sousa, Carina Cury, Sarah Santiloni [UNESP] Nogueira, Célia Regina [UNESP] Correia-de-Sá, Paulo de Oliveira, Miriane [UNESP] |
dc.subject.por.fl_str_mv |
Adenosine ATP Human adipocytes Irisin Obesity Purinergic signaling Thyroid hormones |
topic |
Adenosine ATP Human adipocytes Irisin Obesity Purinergic signaling Thyroid hormones |
description |
The anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:03:31Z 2023-07-29T16:03:31Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbalip.2022.159276 Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023. 1879-2618 1388-1981 http://hdl.handle.net/11449/249576 10.1016/j.bbalip.2022.159276 2-s2.0-85146576762 |
url |
http://dx.doi.org/10.1016/j.bbalip.2022.159276 http://hdl.handle.net/11449/249576 |
identifier_str_mv |
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023. 1879-2618 1388-1981 10.1016/j.bbalip.2022.159276 2-s2.0-85146576762 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128133819269120 |