RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes

Detalhes bibliográficos
Autor(a) principal: Mathias, Lucas Solla [UNESP]
Data de Publicação: 2023
Outros Autores: Herman-de-Sousa, Carina, Cury, Sarah Santiloni [UNESP], Nogueira, Célia Regina [UNESP], Correia-de-Sá, Paulo, de Oliveira, Miriane [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bbalip.2022.159276
http://hdl.handle.net/11449/249576
Resumo: The anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies.
id UNSP_3290f482b7486c64eedf127f90e97d0c
oai_identifier_str oai:repositorio.unesp.br:11449/249576
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytesAdenosineATPHuman adipocytesIrisinObesityPurinergic signalingThyroid hormonesThe anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)European Social FundFundação para a Ciência e a TecnologiaDepartment of Internal Clinic Botucatu Medical School São Paulo State University (UNESP), São PauloLaboratório de Farmacologia e Neurobiologia Departamento de Imuno-Fisiologia e Farmacologia Instituto de Ciências Biomédicas de Abel Salazar Universidade do Porto (ICBAS-UP)Center for Drug Discovery and Innovative Medicines (MedInUP) ICBAS-UPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloDepartment of Internal Clinic Botucatu Medical School São Paulo State University (UNESP), São PauloDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloFAPESP: 2016/03242-3FAPESP: 2020/06763-0CNPq: 409438/2016European Social Fund: NORTE-08-5369-FSE-000011Fundação para a Ciência e a Tecnologia: UIDB/04308/2020Universidade Estadual Paulista (UNESP)Universidade do Porto (ICBAS-UP)ICBAS-UPMathias, Lucas Solla [UNESP]Herman-de-Sousa, CarinaCury, Sarah Santiloni [UNESP]Nogueira, Célia Regina [UNESP]Correia-de-Sá, Paulode Oliveira, Miriane [UNESP]2023-07-29T16:03:31Z2023-07-29T16:03:31Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbalip.2022.159276Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023.1879-26181388-1981http://hdl.handle.net/11449/24957610.1016/j.bbalip.2022.1592762-s2.0-85146576762Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipidsinfo:eu-repo/semantics/openAccess2024-08-14T17:22:48Zoai:repositorio.unesp.br:11449/249576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
title RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
spellingShingle RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
Mathias, Lucas Solla [UNESP]
Adenosine
ATP
Human adipocytes
Irisin
Obesity
Purinergic signaling
Thyroid hormones
title_short RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
title_full RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
title_fullStr RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
title_full_unstemmed RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
title_sort RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes
author Mathias, Lucas Solla [UNESP]
author_facet Mathias, Lucas Solla [UNESP]
Herman-de-Sousa, Carina
Cury, Sarah Santiloni [UNESP]
Nogueira, Célia Regina [UNESP]
Correia-de-Sá, Paulo
de Oliveira, Miriane [UNESP]
author_role author
author2 Herman-de-Sousa, Carina
Cury, Sarah Santiloni [UNESP]
Nogueira, Célia Regina [UNESP]
Correia-de-Sá, Paulo
de Oliveira, Miriane [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade do Porto (ICBAS-UP)
ICBAS-UP
dc.contributor.author.fl_str_mv Mathias, Lucas Solla [UNESP]
Herman-de-Sousa, Carina
Cury, Sarah Santiloni [UNESP]
Nogueira, Célia Regina [UNESP]
Correia-de-Sá, Paulo
de Oliveira, Miriane [UNESP]
dc.subject.por.fl_str_mv Adenosine
ATP
Human adipocytes
Irisin
Obesity
Purinergic signaling
Thyroid hormones
topic Adenosine
ATP
Human adipocytes
Irisin
Obesity
Purinergic signaling
Thyroid hormones
description The anti-obesity thyroid hormone, triiodothyronine (T3), and irisin, an exercise- and/or cold-induced myokine, stimulate thermogenesis and energy consumption while decreasing lipid accumulation. The involvement of ATP signaling in adipocyte cell function and obesity has attracted increasing attention, but the crosstalk between the purinergic signaling cascade and anti-obesity hormones lacks experimental evidence. In this study, we investigated the effects of T3 and irisin in the transcriptomics of membrane-bound purinoceptors, ectonucleotidase enzymes and nucleoside transporters participating in the purinergic signaling in cultured human adipocytes. The RNA-seq analysis revealed that differentiated adipocytes express high amounts of ADORA1, P2RY11, P2RY12, and P2RX6 gene transcripts, along with abundant levels of transcriptional products encoding to purine metabolizing enzymes (ENPP2, ENPP1, NT5E, ADA and ADK) and transporters (SLC29A1, SCL29A2). The transcriptomics of purinergic signaling markers changed in parallel to the upsurge of “browning” adipocyte markers, like UCP1 and P2RX5, after treatment with T3 and irisin. Upregulation of ADORA1, ADORA2A and P2RX4 gene transcription was obtained with irisin, whereas T3 preferentially upregulated NT5E, SLC29A2 and P2RY11 genes. Irisin was more powerful than T3 towards inhibition of the leptin gene transcription, the SCL29A1 gene encoding for the ENT1 transporter, the E-NPP2 (autotaxin) gene, and genes that encode for two ADP-sensitive P2Y receptors, P2RY1 and P2RY12. These findings indicate that anti-obesity irisin and T3 hormones differentially affect the purinergic signaling transcriptomics, which might point towards new directions for the treatment of obesity and related metabolic disorders that are worth to be pursued in future functional studies.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:03:31Z
2023-07-29T16:03:31Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bbalip.2022.159276
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023.
1879-2618
1388-1981
http://hdl.handle.net/11449/249576
10.1016/j.bbalip.2022.159276
2-s2.0-85146576762
url http://dx.doi.org/10.1016/j.bbalip.2022.159276
http://hdl.handle.net/11449/249576
identifier_str_mv Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, v. 1868, n. 4, 2023.
1879-2618
1388-1981
10.1016/j.bbalip.2022.159276
2-s2.0-85146576762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128133819269120