Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12879-017-2562-y http://hdl.handle.net/11449/163011 |
Resumo: | Background: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water. The objective of the present study was to verify whether ATP, as measured by the 3 M (TM) Clean-Trace Water (TM) ATP test, can be used as an alternative tool for presumptive testing for the presence of microorganisms in hospital water. Methods: Water samples (N = 88) were collected from faucets (74) and water purifiers (14) in a university hospital. The sample were filtered by the membrane filter technique (100 mL for bacterial analysis and 100 mL for fungal analysis) and then submitted to ATP bioluminescence assay to the determine quantity of RLU in each sample. In order to compare RLU and the presence of microorganisms, a receiver operating characteristic (ROC) curve was used to calculate sensitivity and specificity (levels higher than 90% were considered significant). In addition, control tests were conducted to compare RLU to the quantities of bacterial and fungal organisms added to distilled water (ANOVA and Tukey's tests; p <= 0.05). This inoculum was compared to RLU emission, and the data were analyzed by calculating the Pearson's correlation coefficient, with a 95% confidence interval. Results: In the present study, 94.3% of the water samples presented bacterial growth. Of these, 15.6% showed heterotrophic bacteria above recommended levels and fungal contamination was detected in 55.6% of samples. Sensitivity and specificity of the samples were not significant (<90%), and the correlation between ATP and the presence of these microorganisms in the samples (hospital water) was not significant, whereas, in distilled water, the results revealed a significant difference (p < 0.0001). Conclusions: These results demonstrated that the ATP test cannot be used as an alternative tool for presumptive assessment of the presence of microorganisms in water. |
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Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sourcesAdenosine triphosphateBacteriaFungiHospitalWaterBackground: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water. The objective of the present study was to verify whether ATP, as measured by the 3 M (TM) Clean-Trace Water (TM) ATP test, can be used as an alternative tool for presumptive testing for the presence of microorganisms in hospital water. Methods: Water samples (N = 88) were collected from faucets (74) and water purifiers (14) in a university hospital. The sample were filtered by the membrane filter technique (100 mL for bacterial analysis and 100 mL for fungal analysis) and then submitted to ATP bioluminescence assay to the determine quantity of RLU in each sample. In order to compare RLU and the presence of microorganisms, a receiver operating characteristic (ROC) curve was used to calculate sensitivity and specificity (levels higher than 90% were considered significant). In addition, control tests were conducted to compare RLU to the quantities of bacterial and fungal organisms added to distilled water (ANOVA and Tukey's tests; p <= 0.05). This inoculum was compared to RLU emission, and the data were analyzed by calculating the Pearson's correlation coefficient, with a 95% confidence interval. Results: In the present study, 94.3% of the water samples presented bacterial growth. Of these, 15.6% showed heterotrophic bacteria above recommended levels and fungal contamination was detected in 55.6% of samples. Sensitivity and specificity of the samples were not significant (<90%), and the correlation between ATP and the presence of these microorganisms in the samples (hospital water) was not significant, whereas, in distilled water, the results revealed a significant difference (p < 0.0001). Conclusions: These results demonstrated that the ATP test cannot be used as an alternative tool for presumptive assessment of the presence of microorganisms in water.Sao Paulo State Univ, Grad Program Microbiol, St Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUniv Fed Mato Grosso do Sul, Master & Doctoral Grad Program Hlth & Dev West Ce, Av Costa & Silva,S-N 79070-900, Campo Grande, Mato Grosso Do, BrazilUniv Fed Mato Grosso do Sul, Master Grad Program Nursing, Av Costa & Silva,S-N 79070-900, Campo Grande, Mato Grosso Do, BrazilUniv Fed Mato Grosso do Sul, Undergrad Program Nursing, Av Ranulpho Marques Leal 3484, BR-79610100 Tres Lagoas, Mato Grosso Do, BrazilUniv Sao Paulo, Ribeirao Preto Coll Nursing, Dept Gen & Specialized Nursing, WHO Collaborating Ctr Nursing Res Dev, Av Bandeirantes 3900, BR-14040902 Ribeirao Preto, SP, BrazilFac Med Sao Jose do Rio Preto, Dept Infect & Parasit Dis, Av Brg Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilAdolfo Lutz Inst, Ctr Reg Lab Sao Jose do Rio Preto, St Rua Alberto Sufredini Bertoni 2325, BR-15060020 Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ, Grad Program Microbiol, St Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilBiomed Central LtdUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade de São Paulo (USP)Fac Med Sao Jose do Rio PretoAdolfo Lutz InstArroyo, Maira Gazzola [UNESP]Ferreira, Adriano MenisFrota, Oleci PereiraRigotti, Marcelo AlessandroAndrade, Denise deBrizzotti, Natalia SeronMacruz Peresi, Jacqueline TanuryCastilho, Elza MariaGottardo de Almeida, Margarete Teresa2018-11-26T17:39:45Z2018-11-26T17:39:45Z2017-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttp://dx.doi.org/10.1186/s12879-017-2562-yBmc Infectious Diseases. London: Biomed Central Ltd, v. 17, 5 p., 2017.1471-2334http://hdl.handle.net/11449/16301110.1186/s12879-017-2562-yWOS:000405719000002WOS000405719000002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Infectious Diseases1,576info:eu-repo/semantics/openAccess2023-12-17T06:18:20Zoai:repositorio.unesp.br:11449/163011Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-17T06:18:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
title |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
spellingShingle |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources Arroyo, Maira Gazzola [UNESP] Adenosine triphosphate Bacteria Fungi Hospital Water |
title_short |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
title_full |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
title_fullStr |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
title_full_unstemmed |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
title_sort |
Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources |
author |
Arroyo, Maira Gazzola [UNESP] |
author_facet |
Arroyo, Maira Gazzola [UNESP] Ferreira, Adriano Menis Frota, Oleci Pereira Rigotti, Marcelo Alessandro Andrade, Denise de Brizzotti, Natalia Seron Macruz Peresi, Jacqueline Tanury Castilho, Elza Maria Gottardo de Almeida, Margarete Teresa |
author_role |
author |
author2 |
Ferreira, Adriano Menis Frota, Oleci Pereira Rigotti, Marcelo Alessandro Andrade, Denise de Brizzotti, Natalia Seron Macruz Peresi, Jacqueline Tanury Castilho, Elza Maria Gottardo de Almeida, Margarete Teresa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade de São Paulo (USP) Fac Med Sao Jose do Rio Preto Adolfo Lutz Inst |
dc.contributor.author.fl_str_mv |
Arroyo, Maira Gazzola [UNESP] Ferreira, Adriano Menis Frota, Oleci Pereira Rigotti, Marcelo Alessandro Andrade, Denise de Brizzotti, Natalia Seron Macruz Peresi, Jacqueline Tanury Castilho, Elza Maria Gottardo de Almeida, Margarete Teresa |
dc.subject.por.fl_str_mv |
Adenosine triphosphate Bacteria Fungi Hospital Water |
topic |
Adenosine triphosphate Bacteria Fungi Hospital Water |
description |
Background: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water. The objective of the present study was to verify whether ATP, as measured by the 3 M (TM) Clean-Trace Water (TM) ATP test, can be used as an alternative tool for presumptive testing for the presence of microorganisms in hospital water. Methods: Water samples (N = 88) were collected from faucets (74) and water purifiers (14) in a university hospital. The sample were filtered by the membrane filter technique (100 mL for bacterial analysis and 100 mL for fungal analysis) and then submitted to ATP bioluminescence assay to the determine quantity of RLU in each sample. In order to compare RLU and the presence of microorganisms, a receiver operating characteristic (ROC) curve was used to calculate sensitivity and specificity (levels higher than 90% were considered significant). In addition, control tests were conducted to compare RLU to the quantities of bacterial and fungal organisms added to distilled water (ANOVA and Tukey's tests; p <= 0.05). This inoculum was compared to RLU emission, and the data were analyzed by calculating the Pearson's correlation coefficient, with a 95% confidence interval. Results: In the present study, 94.3% of the water samples presented bacterial growth. Of these, 15.6% showed heterotrophic bacteria above recommended levels and fungal contamination was detected in 55.6% of samples. Sensitivity and specificity of the samples were not significant (<90%), and the correlation between ATP and the presence of these microorganisms in the samples (hospital water) was not significant, whereas, in distilled water, the results revealed a significant difference (p < 0.0001). Conclusions: These results demonstrated that the ATP test cannot be used as an alternative tool for presumptive assessment of the presence of microorganisms in water. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-30 2018-11-26T17:39:45Z 2018-11-26T17:39:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12879-017-2562-y Bmc Infectious Diseases. London: Biomed Central Ltd, v. 17, 5 p., 2017. 1471-2334 http://hdl.handle.net/11449/163011 10.1186/s12879-017-2562-y WOS:000405719000002 WOS000405719000002.pdf |
url |
http://dx.doi.org/10.1186/s12879-017-2562-y http://hdl.handle.net/11449/163011 |
identifier_str_mv |
Bmc Infectious Diseases. London: Biomed Central Ltd, v. 17, 5 p., 2017. 1471-2334 10.1186/s12879-017-2562-y WOS:000405719000002 WOS000405719000002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Infectious Diseases 1,576 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046986579968000 |