Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pharmaceutics14061149 http://hdl.handle.net/11449/241104 |
Resumo: | Trans-resveratrol can promote various dermatological effects. However, its high crystallinity decreases its solubility and bioavailability. Therefore, solid dispersions have been developed to promote its amorphization; even so, they present as powders, making cutaneous controlled drug delivery unfeasible and an alternative necessary for their incorporation into other systems. Thus, polyvinylpyrrolidone (PVP) films were chosen with the aim of developing a controlled delivery system to treat inflammation and bacterial infections associated with atopic dermatitis. Four formulations were developed: two with solid dispersions (and trans-resveratrol) and two as controls. The films presented with uniformity, as well as bioadhesive and good barrier properties. X-ray diffraction showed that trans-resveratrol did not recrystallize. Fourier-transform infrared spectroscopy (FT-IR) and thermal analysis evidenced good chemical compatibilities. The in vitro release assay showed release values from 82.27 ± 2.60 to 92.81 ± 2.50% (being a prolonged release). In the in vitro retention assay, trans-resveratrol was retained in the skin, over 24 h, from 42.88 to 53.28%. They also had low cytotoxicity over fibroblasts. The in vivo assay showed a reduction in inflammation up to 66%. The films also avoided Staphylococcus aureus’s growth, which worsens atopic dermatitis. According to the results, the developed system is suitable for drug delivery and capable of simultaneously treating inflammation and infections related to atopic dermatitis. |
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Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluationamorphizationanti-inflammatory activityantimicrobial effectcrystallinityskin permeationsolubilitytechnological innovationTrans-resveratrol can promote various dermatological effects. However, its high crystallinity decreases its solubility and bioavailability. Therefore, solid dispersions have been developed to promote its amorphization; even so, they present as powders, making cutaneous controlled drug delivery unfeasible and an alternative necessary for their incorporation into other systems. Thus, polyvinylpyrrolidone (PVP) films were chosen with the aim of developing a controlled delivery system to treat inflammation and bacterial infections associated with atopic dermatitis. Four formulations were developed: two with solid dispersions (and trans-resveratrol) and two as controls. The films presented with uniformity, as well as bioadhesive and good barrier properties. X-ray diffraction showed that trans-resveratrol did not recrystallize. Fourier-transform infrared spectroscopy (FT-IR) and thermal analysis evidenced good chemical compatibilities. The in vitro release assay showed release values from 82.27 ± 2.60 to 92.81 ± 2.50% (being a prolonged release). In the in vitro retention assay, trans-resveratrol was retained in the skin, over 24 h, from 42.88 to 53.28%. They also had low cytotoxicity over fibroblasts. The in vivo assay showed a reduction in inflammation up to 66%. The films also avoided Staphylococcus aureus’s growth, which worsens atopic dermatitis. According to the results, the developed system is suitable for drug delivery and capable of simultaneously treating inflammation and infections related to atopic dermatitis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Drugs and Medicines São Paulo State University, Araraquara-Jaú Hwy. Km 1, Machados, SPDepartment of Clinical Analysis São Paulo State University, Araraquara-Jaú Hwy. Km 1, Machados, SPDepartment of Biotechnology University of Araraquara, Carlos Gomes St., 1338, Centro, SPDepartment of Pharmaceutical Sciences Ponta Grossa State University, General Carlos Cavalcantti Av., 4748, Uvaranas, PRDepartment of Drugs and Medicines São Paulo State University, Araraquara-Jaú Hwy. Km 1, Machados, SPDepartment of Clinical Analysis São Paulo State University, Araraquara-Jaú Hwy. Km 1, Machados, SPFAPESP: 2018/09088-1FAPESP: 2018/23015-7FAPESP: 2018/23357-5FAPESP: 2018/25377-3FAPESP: 2019/ 19817-3FAPESP: 2019/00164-0Universidade Estadual Paulista (UNESP)University of AraraquaraPonta Grossa State UniversityRiccio, Bruno Vincenzo Fiod [UNESP]Do Nascimento, André Luiz Carneiro Soares [UNESP]Meneguin, Andréia Bagliotti [UNESP]Rodero, Camila Fernanda [UNESP]Santos, Kaio Pini [UNESP]Sábio, Rafael Miguel [UNESP]de Annunzio, Sarah Raquel [UNESP]Fontana, Carla Raquel [UNESP]Barud, Hernane da SilvaFerrari, Priscileila ColeratoChorilli, Marlus [UNESP]2023-03-01T20:47:22Z2023-03-01T20:47:22Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pharmaceutics14061149Pharmaceutics, v. 14, n. 6, 2022.1999-4923http://hdl.handle.net/11449/24110410.3390/pharmaceutics140611492-s2.0-85131352574Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticsinfo:eu-repo/semantics/openAccess2024-06-24T13:46:00Zoai:repositorio.unesp.br:11449/241104Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:05:47.662226Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
title |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
spellingShingle |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation Riccio, Bruno Vincenzo Fiod [UNESP] amorphization anti-inflammatory activity antimicrobial effect crystallinity skin permeation solubility technological innovation |
title_short |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
title_full |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
title_fullStr |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
title_full_unstemmed |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
title_sort |
Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation |
author |
Riccio, Bruno Vincenzo Fiod [UNESP] |
author_facet |
Riccio, Bruno Vincenzo Fiod [UNESP] Do Nascimento, André Luiz Carneiro Soares [UNESP] Meneguin, Andréia Bagliotti [UNESP] Rodero, Camila Fernanda [UNESP] Santos, Kaio Pini [UNESP] Sábio, Rafael Miguel [UNESP] de Annunzio, Sarah Raquel [UNESP] Fontana, Carla Raquel [UNESP] Barud, Hernane da Silva Ferrari, Priscileila Colerato Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Do Nascimento, André Luiz Carneiro Soares [UNESP] Meneguin, Andréia Bagliotti [UNESP] Rodero, Camila Fernanda [UNESP] Santos, Kaio Pini [UNESP] Sábio, Rafael Miguel [UNESP] de Annunzio, Sarah Raquel [UNESP] Fontana, Carla Raquel [UNESP] Barud, Hernane da Silva Ferrari, Priscileila Colerato Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Araraquara Ponta Grossa State University |
dc.contributor.author.fl_str_mv |
Riccio, Bruno Vincenzo Fiod [UNESP] Do Nascimento, André Luiz Carneiro Soares [UNESP] Meneguin, Andréia Bagliotti [UNESP] Rodero, Camila Fernanda [UNESP] Santos, Kaio Pini [UNESP] Sábio, Rafael Miguel [UNESP] de Annunzio, Sarah Raquel [UNESP] Fontana, Carla Raquel [UNESP] Barud, Hernane da Silva Ferrari, Priscileila Colerato Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
amorphization anti-inflammatory activity antimicrobial effect crystallinity skin permeation solubility technological innovation |
topic |
amorphization anti-inflammatory activity antimicrobial effect crystallinity skin permeation solubility technological innovation |
description |
Trans-resveratrol can promote various dermatological effects. However, its high crystallinity decreases its solubility and bioavailability. Therefore, solid dispersions have been developed to promote its amorphization; even so, they present as powders, making cutaneous controlled drug delivery unfeasible and an alternative necessary for their incorporation into other systems. Thus, polyvinylpyrrolidone (PVP) films were chosen with the aim of developing a controlled delivery system to treat inflammation and bacterial infections associated with atopic dermatitis. Four formulations were developed: two with solid dispersions (and trans-resveratrol) and two as controls. The films presented with uniformity, as well as bioadhesive and good barrier properties. X-ray diffraction showed that trans-resveratrol did not recrystallize. Fourier-transform infrared spectroscopy (FT-IR) and thermal analysis evidenced good chemical compatibilities. The in vitro release assay showed release values from 82.27 ± 2.60 to 92.81 ± 2.50% (being a prolonged release). In the in vitro retention assay, trans-resveratrol was retained in the skin, over 24 h, from 42.88 to 53.28%. They also had low cytotoxicity over fibroblasts. The in vivo assay showed a reduction in inflammation up to 66%. The films also avoided Staphylococcus aureus’s growth, which worsens atopic dermatitis. According to the results, the developed system is suitable for drug delivery and capable of simultaneously treating inflammation and infections related to atopic dermatitis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-01T20:47:22Z 2023-03-01T20:47:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pharmaceutics14061149 Pharmaceutics, v. 14, n. 6, 2022. 1999-4923 http://hdl.handle.net/11449/241104 10.3390/pharmaceutics14061149 2-s2.0-85131352574 |
url |
http://dx.doi.org/10.3390/pharmaceutics14061149 http://hdl.handle.net/11449/241104 |
identifier_str_mv |
Pharmaceutics, v. 14, n. 6, 2022. 1999-4923 10.3390/pharmaceutics14061149 2-s2.0-85131352574 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129159266828288 |