High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2020.734938 http://hdl.handle.net/11449/196611 |
Resumo: | Tetraploid fish are an important source of diploid gametes for polyploid production, and they can be induced by inhibition of the first mitotic cell division. Dispite its potential, very few protocols regarding tetraploidization and subsequent application to mass production of triploid fish for aquaculture exist. In the yellowtail tetra Astyanax altiparanae, triploids present sterility and increased carcass yield, suggesting the generation of tetraploid for large-scale production of such triploids. In this study, the efficacy of heat shock treatments (40 degrees for 2 min) at 16, 18, 20, 22, 24, and 26 min post-fertilization (mpf) at an incubation temperature of 22 degrees C were compared on tetraploid induction of A. altiparanae. Ploidy status was confirmed by flow cytometry, karyotyping, and nuclear diameter of erythrocytes. Afterwards, sperm parameters and fertility capacity of tetraploid males were evaluated. The results showed that heat shock decreased the hatching rate, especially at 22 (19.1 +/- 9.6%), 24 (16.0 +/- 9.1%), and 26 mpf (10.3 +/- 6.2%) in comparison with the control group (62.2 +/- 7.7%). The flow cytometric analysis showed that the control group and the treatments with heat shock at 16 and 18 mpf presented only diploid individuals. However, from 20 mpf onwards, tetraploid individuals were observed, and the highest percentage arose at 26 mpf (94.55%). When mature, tetraploid males are capable of producing diploid spermatozoa with fertilization capacity, generating a 100% triploid offspring. The results indicate that the heat shock treatment at 26 mpf (40 degrees C for 2 min) is optimum for tetraploid induction in the A. altiparanae. Additionally, tetraploid mature males generated diploid spermatozoa and can be used to mass production of triploid fish. The current data are the first report of tetraploids in family Characidae, presenting an application in basic and applied sciences. |
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High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characinsFishChromosome manipulationHeat-shockPolyploidyTetraploid fish are an important source of diploid gametes for polyploid production, and they can be induced by inhibition of the first mitotic cell division. Dispite its potential, very few protocols regarding tetraploidization and subsequent application to mass production of triploid fish for aquaculture exist. In the yellowtail tetra Astyanax altiparanae, triploids present sterility and increased carcass yield, suggesting the generation of tetraploid for large-scale production of such triploids. In this study, the efficacy of heat shock treatments (40 degrees for 2 min) at 16, 18, 20, 22, 24, and 26 min post-fertilization (mpf) at an incubation temperature of 22 degrees C were compared on tetraploid induction of A. altiparanae. Ploidy status was confirmed by flow cytometry, karyotyping, and nuclear diameter of erythrocytes. Afterwards, sperm parameters and fertility capacity of tetraploid males were evaluated. The results showed that heat shock decreased the hatching rate, especially at 22 (19.1 +/- 9.6%), 24 (16.0 +/- 9.1%), and 26 mpf (10.3 +/- 6.2%) in comparison with the control group (62.2 +/- 7.7%). The flow cytometric analysis showed that the control group and the treatments with heat shock at 16 and 18 mpf presented only diploid individuals. However, from 20 mpf onwards, tetraploid individuals were observed, and the highest percentage arose at 26 mpf (94.55%). When mature, tetraploid males are capable of producing diploid spermatozoa with fertilization capacity, generating a 100% triploid offspring. The results indicate that the heat shock treatment at 26 mpf (40 degrees C for 2 min) is optimum for tetraploid induction in the A. altiparanae. Additionally, tetraploid mature males generated diploid spermatozoa and can be used to mass production of triploid fish. The current data are the first report of tetraploids in family Characidae, presenting an application in basic and applied sciences.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, BrazilUniv Fed Rural Rio de Janeiro, BR-465 Km 7, BR-23897000 Seropedica, RJ, BrazilHokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, JapanSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2010/17429-1FAPESP: 2011/11664-1FAPESP: 2016/12383-0CNPq: 471140/2012-0Elsevier B.V.Universidade Estadual Paulista (Unesp)Chico Mendes Inst Biodivers ConservatUniv Fed Rural Rio de JaneiroHokkaido UnivNascimento, Nivaldo Ferreira do [UNESP]Pereira-Santos, Matheus [UNESP]Levy-Pereira, NycolasMonzani, Paulo SergioNiedzielski, DaianeFujimoto, TakafumiSenhorini, Jose AugustoOkada Nakaghi, Laura Satiko [UNESP]Yasui, George Shigueki2020-12-10T19:50:25Z2020-12-10T19:50:25Z2020-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6http://dx.doi.org/10.1016/j.aquaculture.2020.734938Aquaculture. Amsterdam: Elsevier, v. 520, 6 p., 2020.0044-8486http://hdl.handle.net/11449/19661110.1016/j.aquaculture.2020.734938WOS:000515498200040Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/196611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
title |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
spellingShingle |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins Nascimento, Nivaldo Ferreira do [UNESP] Fish Chromosome manipulation Heat-shock Polyploidy |
title_short |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
title_full |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
title_fullStr |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
title_full_unstemmed |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
title_sort |
High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins |
author |
Nascimento, Nivaldo Ferreira do [UNESP] |
author_facet |
Nascimento, Nivaldo Ferreira do [UNESP] Pereira-Santos, Matheus [UNESP] Levy-Pereira, Nycolas Monzani, Paulo Sergio Niedzielski, Daiane Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] Yasui, George Shigueki |
author_role |
author |
author2 |
Pereira-Santos, Matheus [UNESP] Levy-Pereira, Nycolas Monzani, Paulo Sergio Niedzielski, Daiane Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] Yasui, George Shigueki |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Chico Mendes Inst Biodivers Conservat Univ Fed Rural Rio de Janeiro Hokkaido Univ |
dc.contributor.author.fl_str_mv |
Nascimento, Nivaldo Ferreira do [UNESP] Pereira-Santos, Matheus [UNESP] Levy-Pereira, Nycolas Monzani, Paulo Sergio Niedzielski, Daiane Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] Yasui, George Shigueki |
dc.subject.por.fl_str_mv |
Fish Chromosome manipulation Heat-shock Polyploidy |
topic |
Fish Chromosome manipulation Heat-shock Polyploidy |
description |
Tetraploid fish are an important source of diploid gametes for polyploid production, and they can be induced by inhibition of the first mitotic cell division. Dispite its potential, very few protocols regarding tetraploidization and subsequent application to mass production of triploid fish for aquaculture exist. In the yellowtail tetra Astyanax altiparanae, triploids present sterility and increased carcass yield, suggesting the generation of tetraploid for large-scale production of such triploids. In this study, the efficacy of heat shock treatments (40 degrees for 2 min) at 16, 18, 20, 22, 24, and 26 min post-fertilization (mpf) at an incubation temperature of 22 degrees C were compared on tetraploid induction of A. altiparanae. Ploidy status was confirmed by flow cytometry, karyotyping, and nuclear diameter of erythrocytes. Afterwards, sperm parameters and fertility capacity of tetraploid males were evaluated. The results showed that heat shock decreased the hatching rate, especially at 22 (19.1 +/- 9.6%), 24 (16.0 +/- 9.1%), and 26 mpf (10.3 +/- 6.2%) in comparison with the control group (62.2 +/- 7.7%). The flow cytometric analysis showed that the control group and the treatments with heat shock at 16 and 18 mpf presented only diploid individuals. However, from 20 mpf onwards, tetraploid individuals were observed, and the highest percentage arose at 26 mpf (94.55%). When mature, tetraploid males are capable of producing diploid spermatozoa with fertilization capacity, generating a 100% triploid offspring. The results indicate that the heat shock treatment at 26 mpf (40 degrees C for 2 min) is optimum for tetraploid induction in the A. altiparanae. Additionally, tetraploid mature males generated diploid spermatozoa and can be used to mass production of triploid fish. The current data are the first report of tetraploids in family Characidae, presenting an application in basic and applied sciences. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:50:25Z 2020-12-10T19:50:25Z 2020-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2020.734938 Aquaculture. Amsterdam: Elsevier, v. 520, 6 p., 2020. 0044-8486 http://hdl.handle.net/11449/196611 10.1016/j.aquaculture.2020.734938 WOS:000515498200040 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2020.734938 http://hdl.handle.net/11449/196611 |
identifier_str_mv |
Aquaculture. Amsterdam: Elsevier, v. 520, 6 p., 2020. 0044-8486 10.1016/j.aquaculture.2020.734938 WOS:000515498200040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047504688709632 |