Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1017/S0967199421000125 http://hdl.handle.net/11449/207926 |
Resumo: | Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus. |
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Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservationAquacultureChromosome manipulationFishHeat shockPolyploidySummary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.Lab. of Fish Biotech. Natl. Ctr. for Res. and Conserv. of Contl. Fish Chico Mendes Inst. of Biodiv. Conserv. Rodovia Pref. Euberto Nemésio Pereira de GodoyUniversidade de Saõ Paulo Faculdade de Medicina Veterinária e Ciência Animal Departamento de Reproducąõ AnimalUniversidade Federal Rural de Pernambuco (UFRPE) Unidade Acadêmica de Serra Talhada, Av. Gregório Ferraz Nogueira, s/n,-PEAquaculture Center Saõ Paulo State University Via de Acesso Prof. Paulo Donato Castellane S/nUniversidade Estadual Paulista (UNESP) Departamento de Zoologia SpChina Three Gorges Corporation (CTG)Universidade Estadual Paulista (UNESP) Departamento de Zoologia SpRodovia Pref. Euberto Nemésio Pereira de GodoyFaculdade de Medicina Veterinária e Ciência AnimalUnidade Acadêmica de Serra TalhadaVia de Acesso Prof. Paulo Donato Castellane S/nUniversidade Estadual Paulista (Unesp)China Three Gorges Corporation (CTG)Do Nascimento, Nivaldo FerreiraBertolini, Rafaela Manchin [UNESP]Lopez, Lucia Soares [UNESP]Nakaghi, Laura Satiko OkadaMonzani, Paulo SérgioSenhorini, José Augusto [UNESP]Vianna, Roberto CastroYasui, George Shigueki [UNESP]2021-06-25T11:03:23Z2021-06-25T11:03:23Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S0967199421000125Zygote.1469-87300967-1994http://hdl.handle.net/11449/20792610.1017/S09671994210001252-s2.0-85103703930Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2024-04-09T15:10:54Zoai:repositorio.unesp.br:11449/207926Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:10:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| title |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| spellingShingle |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation Do Nascimento, Nivaldo Ferreira Aquaculture Chromosome manipulation Fish Heat shock Polyploidy |
| title_short |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| title_full |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| title_fullStr |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| title_full_unstemmed |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| title_sort |
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation |
| author |
Do Nascimento, Nivaldo Ferreira |
| author_facet |
Do Nascimento, Nivaldo Ferreira Bertolini, Rafaela Manchin [UNESP] Lopez, Lucia Soares [UNESP] Nakaghi, Laura Satiko Okada Monzani, Paulo Sérgio Senhorini, José Augusto [UNESP] Vianna, Roberto Castro Yasui, George Shigueki [UNESP] |
| author_role |
author |
| author2 |
Bertolini, Rafaela Manchin [UNESP] Lopez, Lucia Soares [UNESP] Nakaghi, Laura Satiko Okada Monzani, Paulo Sérgio Senhorini, José Augusto [UNESP] Vianna, Roberto Castro Yasui, George Shigueki [UNESP] |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Rodovia Pref. Euberto Nemésio Pereira de Godoy Faculdade de Medicina Veterinária e Ciência Animal Unidade Acadêmica de Serra Talhada Via de Acesso Prof. Paulo Donato Castellane S/n Universidade Estadual Paulista (Unesp) China Three Gorges Corporation (CTG) |
| dc.contributor.author.fl_str_mv |
Do Nascimento, Nivaldo Ferreira Bertolini, Rafaela Manchin [UNESP] Lopez, Lucia Soares [UNESP] Nakaghi, Laura Satiko Okada Monzani, Paulo Sérgio Senhorini, José Augusto [UNESP] Vianna, Roberto Castro Yasui, George Shigueki [UNESP] |
| dc.subject.por.fl_str_mv |
Aquaculture Chromosome manipulation Fish Heat shock Polyploidy |
| topic |
Aquaculture Chromosome manipulation Fish Heat shock Polyploidy |
| description |
Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-06-25T11:03:23Z 2021-06-25T11:03:23Z 2021-01-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199421000125 Zygote. 1469-8730 0967-1994 http://hdl.handle.net/11449/207926 10.1017/S0967199421000125 2-s2.0-85103703930 |
| url |
http://dx.doi.org/10.1017/S0967199421000125 http://hdl.handle.net/11449/207926 |
| identifier_str_mv |
Zygote. 1469-8730 0967-1994 10.1017/S0967199421000125 2-s2.0-85103703930 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Zygote |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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