Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates

Detalhes bibliográficos
Autor(a) principal: Paiva, J. B. [UNESP]
Data de Publicação: 2009
Outros Autores: Cavallini, J. S. [UNESP], Silva, M. D. [UNESP], Almeida, M. A. [UNESP], Ângela, H. L. [UNESP], Berchieri Junior, A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-635X2009000400009
http://hdl.handle.net/11449/3107
Resumo: Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was demonstrated that enzymatic restriction of the amplified fliC gene using Hinp1I enzyme can differentiate SG from SP. In the present study, this method was adopted to analyze 14 SP and 22 SG strains, including some strains with atypical results in biochemical tests assessing the utilization of dulcitol and ornithine. The results showed that all SG strains were broken by the enzyme, whereas the 14 SP strains were not.
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spelling Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolatesFliC geneDifferentiationSalmonella GallinarumSalmonella PullorumAlthough Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was demonstrated that enzymatic restriction of the amplified fliC gene using Hinp1I enzyme can differentiate SG from SP. In the present study, this method was adopted to analyze 14 SP and 22 SG strains, including some strains with atypical results in biochemical tests assessing the utilization of dulcitol and ornithine. The results showed that all SG strains were broken by the enzyme, whereas the 14 SP strains were not.UNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia VeterináriaUNESP Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia VeterináriaFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Paiva, J. B. [UNESP]Cavallini, J. S. [UNESP]Silva, M. D. [UNESP]Almeida, M. A. [UNESP]Ângela, H. L. [UNESP]Berchieri Junior, A. [UNESP]2014-05-20T13:16:09Z2014-05-20T13:16:09Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article271-275application/pdfhttp://dx.doi.org/10.1590/S1516-635X2009000400009Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 4, p. 271-275, 2009.1516-635Xhttp://hdl.handle.net/11449/310710.1590/S1516-635X2009000400009S1516-635X2009000400009WOS:000276475000009S1516-635X2009000400009.pdf3508096260678286SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2024-06-07T13:03:18Zoai:repositorio.unesp.br:11449/3107Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:05:46.037099Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
title Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
spellingShingle Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
Paiva, J. B. [UNESP]
FliC gene
Differentiation
Salmonella Gallinarum
Salmonella Pullorum
title_short Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
title_full Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
title_fullStr Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
title_full_unstemmed Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
title_sort Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
author Paiva, J. B. [UNESP]
author_facet Paiva, J. B. [UNESP]
Cavallini, J. S. [UNESP]
Silva, M. D. [UNESP]
Almeida, M. A. [UNESP]
Ângela, H. L. [UNESP]
Berchieri Junior, A. [UNESP]
author_role author
author2 Cavallini, J. S. [UNESP]
Silva, M. D. [UNESP]
Almeida, M. A. [UNESP]
Ângela, H. L. [UNESP]
Berchieri Junior, A. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Paiva, J. B. [UNESP]
Cavallini, J. S. [UNESP]
Silva, M. D. [UNESP]
Almeida, M. A. [UNESP]
Ângela, H. L. [UNESP]
Berchieri Junior, A. [UNESP]
dc.subject.por.fl_str_mv FliC gene
Differentiation
Salmonella Gallinarum
Salmonella Pullorum
topic FliC gene
Differentiation
Salmonella Gallinarum
Salmonella Pullorum
description Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was demonstrated that enzymatic restriction of the amplified fliC gene using Hinp1I enzyme can differentiate SG from SP. In the present study, this method was adopted to analyze 14 SP and 22 SG strains, including some strains with atypical results in biochemical tests assessing the utilization of dulcitol and ornithine. The results showed that all SG strains were broken by the enzyme, whereas the 14 SP strains were not.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2014-05-20T13:16:09Z
2014-05-20T13:16:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-635X2009000400009
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 4, p. 271-275, 2009.
1516-635X
http://hdl.handle.net/11449/3107
10.1590/S1516-635X2009000400009
S1516-635X2009000400009
WOS:000276475000009
S1516-635X2009000400009.pdf
3508096260678286
url http://dx.doi.org/10.1590/S1516-635X2009000400009
http://hdl.handle.net/11449/3107
identifier_str_mv Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 4, p. 271-275, 2009.
1516-635X
10.1590/S1516-635X2009000400009
S1516-635X2009000400009
WOS:000276475000009
S1516-635X2009000400009.pdf
3508096260678286
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Ciência Avícola
0.463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 271-275
application/pdf
dc.publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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