Influência de diferentes soluções irrigadoras associadas à Terapia Fotodinâmica: estudo in vitro da citotoxicidade em fibroblastos L-929

• Autor(a) principal: Maia, Nayara Kívilla do Carmo [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149583; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000867178.pdf
• topic: Tratamento do canal radicular; Fotoquimioterapia; Fibroblastos; Irrigantes do canal radicular; Root canal therapy

Introduction: The photodynamic therapy (PDT) is an aggregate of physics, chemical and biological procedures, that activate the photosensitizer (PS) with light (Laser or Led) to destroy the target cells. The aim of this study was to evaluate in vitro cytotoxicity of PDT, comparing it to different irrigating solutions. Methods: Mouse fibroblasts L-929 were used, held on standard conditions of culture cells (370C with 5% of CO2) which were distributed on: G1-sodium hypochlorite 5%; G2-sodium hypochlorite 2,5%; G3-chlorhexidine 2%; G4-sodium chloride 0,9%; G5-PDT; G6-culture medium (Control). PDT was realized with PS curcumin 500 mg/L and Led wavelength (λ) 480 nm for 4 minutes. Were diluted in culture medium DMEM (1x104 cells), 0,001ml of the solutions to be tested for 6h, 24h and 48h. Three wells were used for each solution containing the fibroblasts which remained incubated for 3h. Cell viability was obtained by colorimetic method MTT. The absorbance was realized with espectrophotometer of λ 570 nm. The results were analysed by ANOVA test with correction of BonFerroni (p<0,05). Results: PDT and sodium chloride 0,9% presented light cytotoxic effect no significant difference statistics compared to the control. There was a significant difference statistics of PDT compared to sodium hypochlorite 5%, 2,5% and chlorhexidine 2% in the period of 6h, 24h and 48h. Conclusions: PDT with curcumin in the concentration used was not cytotoxic unlike the solutions of sodium hypochlorite 5%, 2,5% and chlorhexidine 2%