Citotoxicidade da terapia fotodinâmica e soluções irrigadoras

• Autor(a) principal: Nakao, Jaqueline Midori [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156667; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000901156.pdf
• topic: Tratamento do canal radicular; Irrigantes do canal radicular; Fotoquimioterapia; Citotoxicidade; Root canal therapy

Photodynamic therapy (PDT) is a group of physical, chemical and biological procedures, which occurs after the use of a photosensitizer (PS) activated by light of a specific wavelength (Laser or Led), to destroy the target cell and / or micro-organisms. The aim of this study was to evaluate the cytotoxicity of PDT and the irrigating solutions. L-929 mouse lineage fibroblasts cells were used in this study. The cultures were maintained under standard cell culture conditions (37 ºC and 5% CO2). The solutions were distributed in groups as follow: G1 - Culture medium DMEM (control); G2 - Sodium chloride 0.9%; G3 - 2.5% NaOCl; G4 - 5% NaOCl; G5 - TFD (PS curcumin 1000 mg/L + blue Led λ 480 nm, fluency of 75 J/cm², for 4 minutes); G6 - TFD (PS curcumin 750 mg/L + blue Led λ 480 nm, fluency of 75 J/cm², for 4 minutes). After 6h, 24h and 48h, viable cells were stained with formazan dye MTT, at 37 ºC, 5% CO2 for 4h. The results were statistically analyzed by ANOVA with Bonferroni correction (p<0.05). The control group showed statistically significant difference (p<0.05) with all tested solutions, in all tested periods. There was statistically significant difference about the group of sodium chloride 0.9% with NaOCl 5% and PDT curcumin (1000 mg/L and 750 mg/L) in the period of 6h (p<0.05); with NaOCl (2.5% and 5%) and PDT curcumin (1000 mg/L and 750 mg/L) in the period of 24h (p<0.05); and with PDT curcumin (1000 mg/L) 48h (p<0.05). The NaOCl 2.5% showed less cytotoxic than NaOCl 5% and curcumin 1000 mg/L and 750 mg/L in all experimental periods. The NaOCl 5%, PDT curcumin 1000 mg/L and 750 mg/L were the most cytotoxics solutions (p>0.05) in all periods. There was not statistically significant difference between PDT curcumin 1000 mg/L and 750 mg/L (p>0.05). Conclude that PDT curcumin 1000 mg/L was cytotoxic in L-929 fibroblasts culture. The sodium chloride solution 0.9%, PDT curcumin 750 mg/L, NaOCl (2.5% and 5%) reduced cell viability