Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom

Detalhes bibliográficos
Autor(a) principal: Da Silva, Reinaldo J. [UNESP]
Data de Publicação: 2002
Outros Autores: Silva, Marcia Guimarães da [UNESP], Vilela, Lízia C. [UNESP], Fecchio, Denise [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/0962935029000041
http://hdl.handle.net/11449/66946
Resumo: WE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV.
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spelling Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venomBothrops jararacaCytokinesEhrlich ascites tumorSnake venombatroxobincytokineinterleukin 10interleukin 13interleukin 1alphainterleukin 2interleukin 4interleukin 6tumor necrosis factor alphaanimal experimentanimal modelantineoplastic activitycancer inhibitioncontrolled studyenzyme linked immunosorbent assaymalemouseneutrophilnonhumanperitoneal cavitypriority journaltumor growthAnimalsCarcinoma, Ehrlich TumorCrotalid VenomsInterleukin-10Interleukin-13Interleukin-6MaleMiceTumor Necrosis Factor-alphaAnimaliaBothropsSerpentesWE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV.Departamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Botucatu, São PauloDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, S. Paulo CEP 18618-000Departamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Botucatu, São PauloDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, S. Paulo CEP 18618-000Universidade Estadual Paulista (Unesp)Da Silva, Reinaldo J. [UNESP]Silva, Marcia Guimarães da [UNESP]Vilela, Lízia C. [UNESP]Fecchio, Denise [UNESP]2014-05-27T11:20:29Z2014-05-27T11:20:29Z2002-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article197-201application/pdfhttp://dx.doi.org/10.1080/0962935029000041Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002.0962-9351http://hdl.handle.net/11449/6694610.1080/0962935029000041WOS:0001779848000012-s2.0-00366989392-s2.0-0036698939.pdf79562262807211314940791909535775Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators of Inflammation3.5491,370info:eu-repo/semantics/openAccess2024-09-03T13:14:06Zoai:repositorio.unesp.br:11449/66946Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
title Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
spellingShingle Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
Da Silva, Reinaldo J. [UNESP]
Bothrops jararaca
Cytokines
Ehrlich ascites tumor
Snake venom
batroxobin
cytokine
interleukin 10
interleukin 13
interleukin 1alpha
interleukin 2
interleukin 4
interleukin 6
tumor necrosis factor alpha
animal experiment
animal model
antineoplastic activity
cancer inhibition
controlled study
enzyme linked immunosorbent assay
male
mouse
neutrophil
nonhuman
peritoneal cavity
priority journal
tumor growth
Animals
Carcinoma, Ehrlich Tumor
Crotalid Venoms
Interleukin-10
Interleukin-13
Interleukin-6
Male
Mice
Tumor Necrosis Factor-alpha
Animalia
Bothrops
Serpentes
title_short Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
title_full Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
title_fullStr Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
title_full_unstemmed Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
title_sort Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
author Da Silva, Reinaldo J. [UNESP]
author_facet Da Silva, Reinaldo J. [UNESP]
Silva, Marcia Guimarães da [UNESP]
Vilela, Lízia C. [UNESP]
Fecchio, Denise [UNESP]
author_role author
author2 Silva, Marcia Guimarães da [UNESP]
Vilela, Lízia C. [UNESP]
Fecchio, Denise [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Da Silva, Reinaldo J. [UNESP]
Silva, Marcia Guimarães da [UNESP]
Vilela, Lízia C. [UNESP]
Fecchio, Denise [UNESP]
dc.subject.por.fl_str_mv Bothrops jararaca
Cytokines
Ehrlich ascites tumor
Snake venom
batroxobin
cytokine
interleukin 10
interleukin 13
interleukin 1alpha
interleukin 2
interleukin 4
interleukin 6
tumor necrosis factor alpha
animal experiment
animal model
antineoplastic activity
cancer inhibition
controlled study
enzyme linked immunosorbent assay
male
mouse
neutrophil
nonhuman
peritoneal cavity
priority journal
tumor growth
Animals
Carcinoma, Ehrlich Tumor
Crotalid Venoms
Interleukin-10
Interleukin-13
Interleukin-6
Male
Mice
Tumor Necrosis Factor-alpha
Animalia
Bothrops
Serpentes
topic Bothrops jararaca
Cytokines
Ehrlich ascites tumor
Snake venom
batroxobin
cytokine
interleukin 10
interleukin 13
interleukin 1alpha
interleukin 2
interleukin 4
interleukin 6
tumor necrosis factor alpha
animal experiment
animal model
antineoplastic activity
cancer inhibition
controlled study
enzyme linked immunosorbent assay
male
mouse
neutrophil
nonhuman
peritoneal cavity
priority journal
tumor growth
Animals
Carcinoma, Ehrlich Tumor
Crotalid Venoms
Interleukin-10
Interleukin-13
Interleukin-6
Male
Mice
Tumor Necrosis Factor-alpha
Animalia
Bothrops
Serpentes
description WE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV.
publishDate 2002
dc.date.none.fl_str_mv 2002-08-01
2014-05-27T11:20:29Z
2014-05-27T11:20:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/0962935029000041
Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002.
0962-9351
http://hdl.handle.net/11449/66946
10.1080/0962935029000041
WOS:000177984800001
2-s2.0-0036698939
2-s2.0-0036698939.pdf
7956226280721131
4940791909535775
url http://dx.doi.org/10.1080/0962935029000041
http://hdl.handle.net/11449/66946
identifier_str_mv Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002.
0962-9351
10.1080/0962935029000041
WOS:000177984800001
2-s2.0-0036698939
2-s2.0-0036698939.pdf
7956226280721131
4940791909535775
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mediators of Inflammation
3.549
1,370
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 197-201
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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