Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/0962935029000041 http://hdl.handle.net/11449/66946 |
Resumo: | WE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV. |
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Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venomBothrops jararacaCytokinesEhrlich ascites tumorSnake venombatroxobincytokineinterleukin 10interleukin 13interleukin 1alphainterleukin 2interleukin 4interleukin 6tumor necrosis factor alphaanimal experimentanimal modelantineoplastic activitycancer inhibitioncontrolled studyenzyme linked immunosorbent assaymalemouseneutrophilnonhumanperitoneal cavitypriority journaltumor growthAnimalsCarcinoma, Ehrlich TumorCrotalid VenomsInterleukin-10Interleukin-13Interleukin-6MaleMiceTumor Necrosis Factor-alphaAnimaliaBothropsSerpentesWE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV.Departamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Botucatu, São PauloDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, S. Paulo CEP 18618-000Departamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Botucatu, São PauloDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, S. Paulo CEP 18618-000Universidade Estadual Paulista (Unesp)Da Silva, Reinaldo J. [UNESP]Silva, Marcia Guimarães da [UNESP]Vilela, Lízia C. [UNESP]Fecchio, Denise [UNESP]2014-05-27T11:20:29Z2014-05-27T11:20:29Z2002-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article197-201application/pdfhttp://dx.doi.org/10.1080/0962935029000041Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002.0962-9351http://hdl.handle.net/11449/6694610.1080/0962935029000041WOS:0001779848000012-s2.0-00366989392-s2.0-0036698939.pdf79562262807211314940791909535775Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators of Inflammation3.5491,370info:eu-repo/semantics/openAccess2024-09-03T13:14:06Zoai:repositorio.unesp.br:11449/66946Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
title |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
spellingShingle |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom Da Silva, Reinaldo J. [UNESP] Bothrops jararaca Cytokines Ehrlich ascites tumor Snake venom batroxobin cytokine interleukin 10 interleukin 13 interleukin 1alpha interleukin 2 interleukin 4 interleukin 6 tumor necrosis factor alpha animal experiment animal model antineoplastic activity cancer inhibition controlled study enzyme linked immunosorbent assay male mouse neutrophil nonhuman peritoneal cavity priority journal tumor growth Animals Carcinoma, Ehrlich Tumor Crotalid Venoms Interleukin-10 Interleukin-13 Interleukin-6 Male Mice Tumor Necrosis Factor-alpha Animalia Bothrops Serpentes |
title_short |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
title_full |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
title_fullStr |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
title_full_unstemmed |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
title_sort |
Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom |
author |
Da Silva, Reinaldo J. [UNESP] |
author_facet |
Da Silva, Reinaldo J. [UNESP] Silva, Marcia Guimarães da [UNESP] Vilela, Lízia C. [UNESP] Fecchio, Denise [UNESP] |
author_role |
author |
author2 |
Silva, Marcia Guimarães da [UNESP] Vilela, Lízia C. [UNESP] Fecchio, Denise [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Da Silva, Reinaldo J. [UNESP] Silva, Marcia Guimarães da [UNESP] Vilela, Lízia C. [UNESP] Fecchio, Denise [UNESP] |
dc.subject.por.fl_str_mv |
Bothrops jararaca Cytokines Ehrlich ascites tumor Snake venom batroxobin cytokine interleukin 10 interleukin 13 interleukin 1alpha interleukin 2 interleukin 4 interleukin 6 tumor necrosis factor alpha animal experiment animal model antineoplastic activity cancer inhibition controlled study enzyme linked immunosorbent assay male mouse neutrophil nonhuman peritoneal cavity priority journal tumor growth Animals Carcinoma, Ehrlich Tumor Crotalid Venoms Interleukin-10 Interleukin-13 Interleukin-6 Male Mice Tumor Necrosis Factor-alpha Animalia Bothrops Serpentes |
topic |
Bothrops jararaca Cytokines Ehrlich ascites tumor Snake venom batroxobin cytokine interleukin 10 interleukin 13 interleukin 1alpha interleukin 2 interleukin 4 interleukin 6 tumor necrosis factor alpha animal experiment animal model antineoplastic activity cancer inhibition controlled study enzyme linked immunosorbent assay male mouse neutrophil nonhuman peritoneal cavity priority journal tumor growth Animals Carcinoma, Ehrlich Tumor Crotalid Venoms Interleukin-10 Interleukin-13 Interleukin-6 Male Mice Tumor Necrosis Factor-alpha Animalia Bothrops Serpentes |
description |
WE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-08-01 2014-05-27T11:20:29Z 2014-05-27T11:20:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/0962935029000041 Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002. 0962-9351 http://hdl.handle.net/11449/66946 10.1080/0962935029000041 WOS:000177984800001 2-s2.0-0036698939 2-s2.0-0036698939.pdf 7956226280721131 4940791909535775 |
url |
http://dx.doi.org/10.1080/0962935029000041 http://hdl.handle.net/11449/66946 |
identifier_str_mv |
Mediators of Inflammation, v. 11, n. 4, p. 197-201, 2002. 0962-9351 10.1080/0962935029000041 WOS:000177984800001 2-s2.0-0036698939 2-s2.0-0036698939.pdf 7956226280721131 4940791909535775 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators of Inflammation 3.549 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
197-201 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021354724917248 |