Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3791/61773 http://hdl.handle.net/11449/207720 |
Resumo: | Natural products provide structurally different substances, with a myriad of biological activities. However, the identification and isolation of active compounds from plants are challenging because of the complex plant matrix and time-consuming isolation and identification procedures. Therefore, a stepwise approach for screening natural compounds from plants, including the isolation and identification of potentially active molecules, is presented. It includes the collection of the plant material; preparation and fractionation of crude extracts; chromatography and spectrometry (UHPLC-DAD-HRMS and NMR) approaches for analysis and compounds identification; bioassays (antimicrobial and antibiofilm activities; bacterial adhesion strength to the salivary pellicle and initial glucan matrix treated with selected treatments); and data analysis. The model is simple, reproducible, and allows high-throughput screening of multiple compounds, concentrations, and treatment steps can be consistently controlled. The data obtained provide the foundation for future studies, including formulations with the most active extracts and/or fractions, isolation of molecules, modeling molecules to specific targets in microbial cells and biofilms. For example, one target to control cariogenic biofilm is to inhibit the activity of Streptococcus mutans glucosyltransferases that synthesize the extracellular matrix’ glucans. The inhibition of those enzymes prevents the biofilm build-up, decreasing its virulence. |
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Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental cariesNatural products provide structurally different substances, with a myriad of biological activities. However, the identification and isolation of active compounds from plants are challenging because of the complex plant matrix and time-consuming isolation and identification procedures. Therefore, a stepwise approach for screening natural compounds from plants, including the isolation and identification of potentially active molecules, is presented. It includes the collection of the plant material; preparation and fractionation of crude extracts; chromatography and spectrometry (UHPLC-DAD-HRMS and NMR) approaches for analysis and compounds identification; bioassays (antimicrobial and antibiofilm activities; bacterial adhesion strength to the salivary pellicle and initial glucan matrix treated with selected treatments); and data analysis. The model is simple, reproducible, and allows high-throughput screening of multiple compounds, concentrations, and treatment steps can be consistently controlled. The data obtained provide the foundation for future studies, including formulations with the most active extracts and/or fractions, isolation of molecules, modeling molecules to specific targets in microbial cells and biofilms. For example, one target to control cariogenic biofilm is to inhibit the activity of Streptococcus mutans glucosyltransferases that synthesize the extracellular matrix’ glucans. The inhibition of those enzymes prevents the biofilm build-up, decreasing its virulence.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Faculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP) Department of Physics and Chemistry University of São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)FAPESP: 2011/21440–3FAPESP: 2012/21921– 4FAPESP: 2013/07600–3FAPESP: 2014/50926–0FAPESP: 2017/07408–6FAPESP: 2019/23175-7FAPESP: 465637/2014–0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Ribeiro, Sabrina M. [UNESP]Fratucelli, Érick D. O. [UNESP]Fernandes, Júlia M. [UNESP]Bueno, Paula C. P. [UNESP]Cavalheiro, Alberto José [UNESP]Klein, Marlise I. [UNESP]2021-06-25T10:59:52Z2021-06-25T10:59:52Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3791/61773Journal of Visualized Experiments, v. 2021, n. 169, 2021.1940-087Xhttp://hdl.handle.net/11449/20772010.3791/617732-s2.0-85105690789Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Visualized Experimentsinfo:eu-repo/semantics/openAccess2021-10-23T17:45:56Zoai:repositorio.unesp.br:11449/207720Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:45:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
title |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
spellingShingle |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries Ribeiro, Sabrina M. [UNESP] |
title_short |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
title_full |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
title_fullStr |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
title_full_unstemmed |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
title_sort |
Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries |
author |
Ribeiro, Sabrina M. [UNESP] |
author_facet |
Ribeiro, Sabrina M. [UNESP] Fratucelli, Érick D. O. [UNESP] Fernandes, Júlia M. [UNESP] Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
author_role |
author |
author2 |
Fratucelli, Érick D. O. [UNESP] Fernandes, Júlia M. [UNESP] Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ribeiro, Sabrina M. [UNESP] Fratucelli, Érick D. O. [UNESP] Fernandes, Júlia M. [UNESP] Bueno, Paula C. P. [UNESP] Cavalheiro, Alberto José [UNESP] Klein, Marlise I. [UNESP] |
description |
Natural products provide structurally different substances, with a myriad of biological activities. However, the identification and isolation of active compounds from plants are challenging because of the complex plant matrix and time-consuming isolation and identification procedures. Therefore, a stepwise approach for screening natural compounds from plants, including the isolation and identification of potentially active molecules, is presented. It includes the collection of the plant material; preparation and fractionation of crude extracts; chromatography and spectrometry (UHPLC-DAD-HRMS and NMR) approaches for analysis and compounds identification; bioassays (antimicrobial and antibiofilm activities; bacterial adhesion strength to the salivary pellicle and initial glucan matrix treated with selected treatments); and data analysis. The model is simple, reproducible, and allows high-throughput screening of multiple compounds, concentrations, and treatment steps can be consistently controlled. The data obtained provide the foundation for future studies, including formulations with the most active extracts and/or fractions, isolation of molecules, modeling molecules to specific targets in microbial cells and biofilms. For example, one target to control cariogenic biofilm is to inhibit the activity of Streptococcus mutans glucosyltransferases that synthesize the extracellular matrix’ glucans. The inhibition of those enzymes prevents the biofilm build-up, decreasing its virulence. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:52Z 2021-06-25T10:59:52Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3791/61773 Journal of Visualized Experiments, v. 2021, n. 169, 2021. 1940-087X http://hdl.handle.net/11449/207720 10.3791/61773 2-s2.0-85105690789 |
url |
http://dx.doi.org/10.3791/61773 http://hdl.handle.net/11449/207720 |
identifier_str_mv |
Journal of Visualized Experiments, v. 2021, n. 169, 2021. 1940-087X 10.3791/61773 2-s2.0-85105690789 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Visualized Experiments |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964990746132480 |