Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-04341-5 http://hdl.handle.net/11449/223282 |
Resumo: | Objectives: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. Materials and methods: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey’s test were used to analyze the data (α = 0.05). Results: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. Conclusions: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells’ viability. Clinical relevance: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients’ cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness. |
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Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materialsCandida albicansCinnamomum verumDenture stomatitisElectrospinningNanofibersObjectives: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. Materials and methods: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey’s test were used to analyze the data (α = 0.05). Results: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. Conclusions: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells’ viability. Clinical relevance: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients’ cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness.Department of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry, 1011 N. University (Room 5223)Department of Restorative Dentistry School of Dentistry Federal University of Pelotas, RSDepartment of Physiology and Pathology University Estadual Paulista – UNESP, SPPost-Graduate Program in Oral Science Faculty of Dentistry Federal University of Santa Maria (UFSM), Rio Grande Do Sul StateDepartment of Periodontics and Oral Medicine University of Michigan School of DentistryDepartment of Physiology and Pathology University Estadual Paulista – UNESP, SPUniversity of Michigan School of DentistryFederal University of PelotasUniversidade Estadual Paulista (UNESP)Universidade Federal de Sergipe (UFS)Ribeiro, Juliana SilvaBordini, Ester Alves Ferreira [UNESP]Pereira, Gabriel Kalil RochaPolasani, Rohitha RaoSquarize, Cristiane HelenaKantorski, Karla ZaniniValandro, Luiz FelipeBottino, Marco Cícero2022-04-28T19:49:41Z2022-04-28T19:49:41Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-021-04341-5Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/22328210.1007/s00784-021-04341-52-s2.0-85123060681Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2022-04-28T19:49:41Zoai:repositorio.unesp.br:11449/223282Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:49:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
title |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
spellingShingle |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials Ribeiro, Juliana Silva Candida albicans Cinnamomum verum Denture stomatitis Electrospinning Nanofibers |
title_short |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
title_full |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
title_fullStr |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
title_full_unstemmed |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
title_sort |
Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials |
author |
Ribeiro, Juliana Silva |
author_facet |
Ribeiro, Juliana Silva Bordini, Ester Alves Ferreira [UNESP] Pereira, Gabriel Kalil Rocha Polasani, Rohitha Rao Squarize, Cristiane Helena Kantorski, Karla Zanini Valandro, Luiz Felipe Bottino, Marco Cícero |
author_role |
author |
author2 |
Bordini, Ester Alves Ferreira [UNESP] Pereira, Gabriel Kalil Rocha Polasani, Rohitha Rao Squarize, Cristiane Helena Kantorski, Karla Zanini Valandro, Luiz Felipe Bottino, Marco Cícero |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Michigan School of Dentistry Federal University of Pelotas Universidade Estadual Paulista (UNESP) Universidade Federal de Sergipe (UFS) |
dc.contributor.author.fl_str_mv |
Ribeiro, Juliana Silva Bordini, Ester Alves Ferreira [UNESP] Pereira, Gabriel Kalil Rocha Polasani, Rohitha Rao Squarize, Cristiane Helena Kantorski, Karla Zanini Valandro, Luiz Felipe Bottino, Marco Cícero |
dc.subject.por.fl_str_mv |
Candida albicans Cinnamomum verum Denture stomatitis Electrospinning Nanofibers |
topic |
Candida albicans Cinnamomum verum Denture stomatitis Electrospinning Nanofibers |
description |
Objectives: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. Materials and methods: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey’s test were used to analyze the data (α = 0.05). Results: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. Conclusions: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells’ viability. Clinical relevance: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients’ cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:49:41Z 2022-04-28T19:49:41Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-04341-5 Clinical Oral Investigations. 1436-3771 1432-6981 http://hdl.handle.net/11449/223282 10.1007/s00784-021-04341-5 2-s2.0-85123060681 |
url |
http://dx.doi.org/10.1007/s00784-021-04341-5 http://hdl.handle.net/11449/223282 |
identifier_str_mv |
Clinical Oral Investigations. 1436-3771 1432-6981 10.1007/s00784-021-04341-5 2-s2.0-85123060681 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965303279452160 |