Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cimid.2018.01.003 http://hdl.handle.net/11449/175981 |
Resumo: | Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer. |
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Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasisApoptosisCanis familiarisCD14Cellular immunityFlow cytometryMacrophagesNitric oxideParasite loadVisceral leishmaniasisZoonosisNitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer.Department of Clinical Care Surgery and Animal Reproduction Laboratory of Cellular Immunology Faculty of Veterinary Medicine of Araçatuba Universidade Estadual Paulista “Julio de Mesquita Filho” FMVA/UNESP, Rua Clovis Pestana, 793, AraçatubaDepartment of Clinical Care Surgery and Animal Reproduction Laboratory of Cellular Immunology Faculty of Veterinary Medicine of Araçatuba Universidade Estadual Paulista “Julio de Mesquita Filho” FMVA/UNESP, Rua Clovis Pestana, 793, AraçatubaUniversidade Estadual Paulista (Unesp)de Martini, Cleber Costa [UNESP]de Andrade, Jéssica Thomé [UNESP]de Almeida, Stéfani Karin Martiniano [UNESP]Oliveira Silva, Kathlenn Liezbeth [UNESP]de Rezende Eugenio, Flavia [UNESP]dos Santos, Paulo Sergio Patto [UNESP]de Lima, Valéria Marçal Felix [UNESP]2018-12-11T17:18:25Z2018-12-11T17:18:25Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1016/j.cimid.2018.01.003Comparative Immunology, Microbiology and Infectious Diseases, v. 57, p. 1-7.1878-16670147-9571http://hdl.handle.net/11449/17598110.1016/j.cimid.2018.01.0032-s2.0-850429216972-s2.0-85042921697.pdf53319389628966640000-0002-8535-5569Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Immunology, Microbiology and Infectious Diseases0,877info:eu-repo/semantics/openAccess2024-09-04T18:04:10Zoai:repositorio.unesp.br:11449/175981Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
title |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
spellingShingle |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis de Martini, Cleber Costa [UNESP] Apoptosis Canis familiaris CD14 Cellular immunity Flow cytometry Macrophages Nitric oxide Parasite load Visceral leishmaniasis Zoonosis |
title_short |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
title_full |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
title_fullStr |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
title_full_unstemmed |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
title_sort |
Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis |
author |
de Martini, Cleber Costa [UNESP] |
author_facet |
de Martini, Cleber Costa [UNESP] de Andrade, Jéssica Thomé [UNESP] de Almeida, Stéfani Karin Martiniano [UNESP] Oliveira Silva, Kathlenn Liezbeth [UNESP] de Rezende Eugenio, Flavia [UNESP] dos Santos, Paulo Sergio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author_role |
author |
author2 |
de Andrade, Jéssica Thomé [UNESP] de Almeida, Stéfani Karin Martiniano [UNESP] Oliveira Silva, Kathlenn Liezbeth [UNESP] de Rezende Eugenio, Flavia [UNESP] dos Santos, Paulo Sergio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Martini, Cleber Costa [UNESP] de Andrade, Jéssica Thomé [UNESP] de Almeida, Stéfani Karin Martiniano [UNESP] Oliveira Silva, Kathlenn Liezbeth [UNESP] de Rezende Eugenio, Flavia [UNESP] dos Santos, Paulo Sergio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Canis familiaris CD14 Cellular immunity Flow cytometry Macrophages Nitric oxide Parasite load Visceral leishmaniasis Zoonosis |
topic |
Apoptosis Canis familiaris CD14 Cellular immunity Flow cytometry Macrophages Nitric oxide Parasite load Visceral leishmaniasis Zoonosis |
description |
Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:25Z 2018-12-11T17:18:25Z 2018-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cimid.2018.01.003 Comparative Immunology, Microbiology and Infectious Diseases, v. 57, p. 1-7. 1878-1667 0147-9571 http://hdl.handle.net/11449/175981 10.1016/j.cimid.2018.01.003 2-s2.0-85042921697 2-s2.0-85042921697.pdf 5331938962896664 0000-0002-8535-5569 |
url |
http://dx.doi.org/10.1016/j.cimid.2018.01.003 http://hdl.handle.net/11449/175981 |
identifier_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases, v. 57, p. 1-7. 1878-1667 0147-9571 10.1016/j.cimid.2018.01.003 2-s2.0-85042921697 2-s2.0-85042921697.pdf 5331938962896664 0000-0002-8535-5569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases 0,877 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021406659837952 |