Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay

Detalhes bibliográficos
Autor(a) principal: Miyaji, CK
Data de Publicação: 2004
Outros Autores: Jordão, BQ, Ribeiro, LR [UNESP], Eira, Augusto Ferreira da [UNESP], Cólus, IMS
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572004000100018
http://hdl.handle.net/11449/6104
Resumo: The mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at 22 ± 2 and 60 °C showed moderate genotoxic activity. To test the protective effect of the three concentrations of the extracts against the genotoxicity induced by methyl methanesulfonate, three protocols were used: pre-treatment, simultaneous-treatment and post-treatment. Treatments were repeated for all combinations of preparation temperature and concentration. Two extracts (22 ± 2 °C 1.0 mg/mL (simultaneous-treatment) and 4 °C 0.5 mg/mL (post-treatment)) showed antigenotoxic activity.
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spelling Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assayshiitakeComet assayHEp-2 cellsgenotoxicityantigenotoxicityThe mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at 22 ± 2 and 60 °C showed moderate genotoxic activity. To test the protective effect of the three concentrations of the extracts against the genotoxicity induced by methyl methanesulfonate, three protocols were used: pre-treatment, simultaneous-treatment and post-treatment. Treatments were repeated for all combinations of preparation temperature and concentration. Two extracts (22 ± 2 °C 1.0 mg/mL (simultaneous-treatment) and 4 °C 0.5 mg/mL (post-treatment)) showed antigenotoxic activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual de Londrina (UEL)Universidade Estadual PaulistaUniversidade Luterana do BrasilUniversidade Estadual PaulistaSociedade Brasileira de GenéticaUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Universidade Luterana do BrasilMiyaji, CKJordão, BQRibeiro, LR [UNESP]Eira, Augusto Ferreira da [UNESP]Cólus, IMS2014-05-20T13:21:15Z2014-05-20T13:21:15Z2004-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-114application/pdfhttp://dx.doi.org/10.1590/S1415-47572004000100018Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 27, n. 1, p. 108-114, 2004.1415-4757http://hdl.handle.net/11449/610410.1590/S1415-47572004000100018S1415-47572004000100018S1415-47572004000100018.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-26T06:06:11Zoai:repositorio.unesp.br:11449/6104Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:00:23.114548Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
title Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
spellingShingle Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
Miyaji, CK
shiitake
Comet assay
HEp-2 cells
genotoxicity
antigenotoxicity
title_short Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
title_full Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
title_fullStr Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
title_full_unstemmed Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
title_sort Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
author Miyaji, CK
author_facet Miyaji, CK
Jordão, BQ
Ribeiro, LR [UNESP]
Eira, Augusto Ferreira da [UNESP]
Cólus, IMS
author_role author
author2 Jordão, BQ
Ribeiro, LR [UNESP]
Eira, Augusto Ferreira da [UNESP]
Cólus, IMS
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (Unesp)
Universidade Luterana do Brasil
dc.contributor.author.fl_str_mv Miyaji, CK
Jordão, BQ
Ribeiro, LR [UNESP]
Eira, Augusto Ferreira da [UNESP]
Cólus, IMS
dc.subject.por.fl_str_mv shiitake
Comet assay
HEp-2 cells
genotoxicity
antigenotoxicity
topic shiitake
Comet assay
HEp-2 cells
genotoxicity
antigenotoxicity
description The mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at 22 ± 2 and 60 °C showed moderate genotoxic activity. To test the protective effect of the three concentrations of the extracts against the genotoxicity induced by methyl methanesulfonate, three protocols were used: pre-treatment, simultaneous-treatment and post-treatment. Treatments were repeated for all combinations of preparation temperature and concentration. Two extracts (22 ± 2 °C 1.0 mg/mL (simultaneous-treatment) and 4 °C 0.5 mg/mL (post-treatment)) showed antigenotoxic activity.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
2014-05-20T13:21:15Z
2014-05-20T13:21:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572004000100018
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 27, n. 1, p. 108-114, 2004.
1415-4757
http://hdl.handle.net/11449/6104
10.1590/S1415-47572004000100018
S1415-47572004000100018
S1415-47572004000100018.pdf
url http://dx.doi.org/10.1590/S1415-47572004000100018
http://hdl.handle.net/11449/6104
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 27, n. 1, p. 108-114, 2004.
1415-4757
10.1590/S1415-47572004000100018
S1415-47572004000100018
S1415-47572004000100018.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 108-114
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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