Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jme/tjw053 http://hdl.handle.net/11449/161700 |
Resumo: | Aedes aegypti (L.) resistance to pyrethroids was recorded in Brazil few years after its introduction as the adulticide in the National Dengue Control Program campaigns. Altered susceptibility to pyrethroids had been reported in the state of Sergipe, northeast Brazil, through biological assays, even before its use against Ae. aegypti in the state. Metabolic and target-site resistance mechanisms were also revealed in samples from Aracaju, the capital of Sergipe. Herein, we investigated the presence and distribution of the kdr mutation V1016I(kdr) in Ae. aegypti populations from different municipalities of the state. Aedes aegypti eggs were collected from seven municipalities located in areas showing different climatic types and infestation levels. Approximately 20 Ae. aegypti females from each municipality (total of 135 subjects) were individually submitted to allele-specific polymerase chain reaction (AS-PCR) for the 1016 site of the voltage-gated sodium channel (NaV). The V1016I(kdr) mutation was found in subjects from all the municipalities under study with a high frequency of heterozygotes in several locations. Homozygous recessive subjects (resistant kdr genotype) were found only in one municipality. The results suggest a wide distribution of the V1016I(kdr) mutation in the northeast Brazil, which indicates urgent need for monitoring the effectiveness of the pyrethroids currently used for vector control. |
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Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast BrazilAedes aegyptikdr mutationpyrethroid resistancesodium channelAedes aegypti (L.) resistance to pyrethroids was recorded in Brazil few years after its introduction as the adulticide in the National Dengue Control Program campaigns. Altered susceptibility to pyrethroids had been reported in the state of Sergipe, northeast Brazil, through biological assays, even before its use against Ae. aegypti in the state. Metabolic and target-site resistance mechanisms were also revealed in samples from Aracaju, the capital of Sergipe. Herein, we investigated the presence and distribution of the kdr mutation V1016I(kdr) in Ae. aegypti populations from different municipalities of the state. Aedes aegypti eggs were collected from seven municipalities located in areas showing different climatic types and infestation levels. Approximately 20 Ae. aegypti females from each municipality (total of 135 subjects) were individually submitted to allele-specific polymerase chain reaction (AS-PCR) for the 1016 site of the voltage-gated sodium channel (NaV). The V1016I(kdr) mutation was found in subjects from all the municipalities under study with a high frequency of heterozygotes in several locations. Homozygous recessive subjects (resistant kdr genotype) were found only in one municipality. The results suggest a wide distribution of the V1016I(kdr) mutation in the northeast Brazil, which indicates urgent need for monitoring the effectiveness of the pyrethroids currently used for vector control.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sergipe, Dept Morfol, Lab Entomol & Parasitol Trop, Sao Cristovao, Sergipe, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Botucatu, SP, BrazilUniv Fed Sergipe, Dept Farm, Lab Quim Med, Sao Cristovao, Sergipe, BrazilIOC Fiocruz, Lab Fisiol & Controle Artropodes Vetores, Rio De Janeiro, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Botucatu, SP, BrazilCAPES: CAPES/FAPITEC 2345/2012Oxford Univ Press IncUniversidade Federal de Sergipe (UFS)Universidade Estadual Paulista (Unesp)IOC FiocruzDolabella, S. S.Santos, R. L. C.Silva, M. C. N.Steffler, L. M.Ribolla, P. E. M. [UNESP]Cavalcanti, S. C. H.Jain, S.Martins, A. J.2018-11-26T16:48:17Z2018-11-26T16:48:17Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article967-971application/pdfhttp://dx.doi.org/10.1093/jme/tjw053Journal Of Medical Entomology. Cary: Oxford Univ Press Inc, v. 53, n. 4, p. 967-971, 2016.0022-2585http://hdl.handle.net/11449/16170010.1093/jme/tjw053WOS:000379204800032WOS000379204800032.pdf35771497484568800000-0001-8735-6090Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Medical Entomology0,962info:eu-repo/semantics/openAccess2024-01-19T06:28:09Zoai:repositorio.unesp.br:11449/161700Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-19T06:28:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
title |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
spellingShingle |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil Dolabella, S. S. Aedes aegypti kdr mutation pyrethroid resistance sodium channel |
title_short |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
title_full |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
title_fullStr |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
title_full_unstemmed |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
title_sort |
Detection and Distribution of V1016I(kdr) Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil |
author |
Dolabella, S. S. |
author_facet |
Dolabella, S. S. Santos, R. L. C. Silva, M. C. N. Steffler, L. M. Ribolla, P. E. M. [UNESP] Cavalcanti, S. C. H. Jain, S. Martins, A. J. |
author_role |
author |
author2 |
Santos, R. L. C. Silva, M. C. N. Steffler, L. M. Ribolla, P. E. M. [UNESP] Cavalcanti, S. C. H. Jain, S. Martins, A. J. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Sergipe (UFS) Universidade Estadual Paulista (Unesp) IOC Fiocruz |
dc.contributor.author.fl_str_mv |
Dolabella, S. S. Santos, R. L. C. Silva, M. C. N. Steffler, L. M. Ribolla, P. E. M. [UNESP] Cavalcanti, S. C. H. Jain, S. Martins, A. J. |
dc.subject.por.fl_str_mv |
Aedes aegypti kdr mutation pyrethroid resistance sodium channel |
topic |
Aedes aegypti kdr mutation pyrethroid resistance sodium channel |
description |
Aedes aegypti (L.) resistance to pyrethroids was recorded in Brazil few years after its introduction as the adulticide in the National Dengue Control Program campaigns. Altered susceptibility to pyrethroids had been reported in the state of Sergipe, northeast Brazil, through biological assays, even before its use against Ae. aegypti in the state. Metabolic and target-site resistance mechanisms were also revealed in samples from Aracaju, the capital of Sergipe. Herein, we investigated the presence and distribution of the kdr mutation V1016I(kdr) in Ae. aegypti populations from different municipalities of the state. Aedes aegypti eggs were collected from seven municipalities located in areas showing different climatic types and infestation levels. Approximately 20 Ae. aegypti females from each municipality (total of 135 subjects) were individually submitted to allele-specific polymerase chain reaction (AS-PCR) for the 1016 site of the voltage-gated sodium channel (NaV). The V1016I(kdr) mutation was found in subjects from all the municipalities under study with a high frequency of heterozygotes in several locations. Homozygous recessive subjects (resistant kdr genotype) were found only in one municipality. The results suggest a wide distribution of the V1016I(kdr) mutation in the northeast Brazil, which indicates urgent need for monitoring the effectiveness of the pyrethroids currently used for vector control. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-11-26T16:48:17Z 2018-11-26T16:48:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jme/tjw053 Journal Of Medical Entomology. Cary: Oxford Univ Press Inc, v. 53, n. 4, p. 967-971, 2016. 0022-2585 http://hdl.handle.net/11449/161700 10.1093/jme/tjw053 WOS:000379204800032 WOS000379204800032.pdf 3577149748456880 0000-0001-8735-6090 |
url |
http://dx.doi.org/10.1093/jme/tjw053 http://hdl.handle.net/11449/161700 |
identifier_str_mv |
Journal Of Medical Entomology. Cary: Oxford Univ Press Inc, v. 53, n. 4, p. 967-971, 2016. 0022-2585 10.1093/jme/tjw053 WOS:000379204800032 WOS000379204800032.pdf 3577149748456880 0000-0001-8735-6090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Medical Entomology 0,962 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
967-971 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965662450286592 |