Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2527/jas2016-0293 http://hdl.handle.net/11449/161494 |
Resumo: | Boar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Furthermore, seminal plasma (SP) can increase the cryotolerance of boar spermatozoa, and in other domestic species, it has the ability to reverse cryopreservation damage. This study aimed to evaluate the effects of boar SP arising from the whole sperm-rich ejaculate fraction (SP-SRF) on the integrity, stability, and peroxidation of sperm membranes after thawing. Each ejaculate (n = 24) was divided among 4 treatments: control (CT), centrifuged and suspended in autologous SP-SRF (CS), centrifuged with withdrawn SP-SRF (CW), and post-thawed SP arising from the whole sperm-rich fraction addition to CW (CWSP). After thawing, all treatments were incubated for 5, 60, and 120 min and were analyzed for membrane integrity, fluidity, and peroxidation by flow cytometer. The absence of SP-SRF increased the lipid disorder (P < 0.05) but had no effect on lipid peroxidation (P > 0.05) or membrane integrity (P > 0.05). However, the increase in lipid disorder by withdrawal of SP-SRF was reversed by SP-SRF addition (P < 0.05) to the post-thawing medium, whereas plasma and acrosomal membrane integrity (P > 0.05) and lipid peroxidation (P > 0.05) were unchanged. In conclusion, despite the centrifugation effects, the addition of SP arising from the whole sperm-rich fraction to post-thawed boar semen decreased sperm lipid disorder without an influence of the sperm membrane integrity and peroxidation. |
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Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawinglipid disorderlipid peroxidationmembrane integrityseminal plasmaspermatozoaswineBoar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Furthermore, seminal plasma (SP) can increase the cryotolerance of boar spermatozoa, and in other domestic species, it has the ability to reverse cryopreservation damage. This study aimed to evaluate the effects of boar SP arising from the whole sperm-rich ejaculate fraction (SP-SRF) on the integrity, stability, and peroxidation of sperm membranes after thawing. Each ejaculate (n = 24) was divided among 4 treatments: control (CT), centrifuged and suspended in autologous SP-SRF (CS), centrifuged with withdrawn SP-SRF (CW), and post-thawed SP arising from the whole sperm-rich fraction addition to CW (CWSP). After thawing, all treatments were incubated for 5, 60, and 120 min and were analyzed for membrane integrity, fluidity, and peroxidation by flow cytometer. The absence of SP-SRF increased the lipid disorder (P < 0.05) but had no effect on lipid peroxidation (P > 0.05) or membrane integrity (P > 0.05). However, the increase in lipid disorder by withdrawal of SP-SRF was reversed by SP-SRF addition (P < 0.05) to the post-thawing medium, whereas plasma and acrosomal membrane integrity (P > 0.05) and lipid peroxidation (P > 0.05) were unchanged. In conclusion, despite the centrifugation effects, the addition of SP arising from the whole sperm-rich fraction to post-thawed boar semen decreased sperm lipid disorder without an influence of the sperm membrane integrity and peroxidation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl & Technol Swine Embryos, BR-13635900 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, Lab Swine Res, BR-13635900 Pirassununga, SP, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Vet Med, BR-13635900 Pirassununga, SP, BrazilSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilFAPESP: 2011/23484-8FAPESP: 2013/08070-8Amer Soc Animal ScienceUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Torres, M. A.Ravagnani, G. M.Leal, D. F.Martins, S. M. M. K.Muro, B. B. D.Meirelles, F. V.Papa, F. O. [UNESP]Dell'aqua Junior, J. A. [UNESP]Alvarenga, M. A. [UNESP]Moretti, A. S.De Andrade, A. F. C.2018-11-26T16:32:57Z2018-11-26T16:32:57Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1906-1912application/pdfhttp://dx.doi.org/10.2527/jas2016-0293Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 5, p. 1906-1912, 2016.0021-8812http://hdl.handle.net/11449/16149410.2527/jas2016-0293WOS:000375783300014WOS000375783300014.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Animal Science0,848info:eu-repo/semantics/openAccess2024-09-09T14:06:05Zoai:repositorio.unesp.br:11449/161494Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
title |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
spellingShingle |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing Torres, M. A. lipid disorder lipid peroxidation membrane integrity seminal plasma spermatozoa swine |
title_short |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
title_full |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
title_fullStr |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
title_full_unstemmed |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
title_sort |
Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing |
author |
Torres, M. A. |
author_facet |
Torres, M. A. Ravagnani, G. M. Leal, D. F. Martins, S. M. M. K. Muro, B. B. D. Meirelles, F. V. Papa, F. O. [UNESP] Dell'aqua Junior, J. A. [UNESP] Alvarenga, M. A. [UNESP] Moretti, A. S. De Andrade, A. F. C. |
author_role |
author |
author2 |
Ravagnani, G. M. Leal, D. F. Martins, S. M. M. K. Muro, B. B. D. Meirelles, F. V. Papa, F. O. [UNESP] Dell'aqua Junior, J. A. [UNESP] Alvarenga, M. A. [UNESP] Moretti, A. S. De Andrade, A. F. C. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Torres, M. A. Ravagnani, G. M. Leal, D. F. Martins, S. M. M. K. Muro, B. B. D. Meirelles, F. V. Papa, F. O. [UNESP] Dell'aqua Junior, J. A. [UNESP] Alvarenga, M. A. [UNESP] Moretti, A. S. De Andrade, A. F. C. |
dc.subject.por.fl_str_mv |
lipid disorder lipid peroxidation membrane integrity seminal plasma spermatozoa swine |
topic |
lipid disorder lipid peroxidation membrane integrity seminal plasma spermatozoa swine |
description |
Boar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Furthermore, seminal plasma (SP) can increase the cryotolerance of boar spermatozoa, and in other domestic species, it has the ability to reverse cryopreservation damage. This study aimed to evaluate the effects of boar SP arising from the whole sperm-rich ejaculate fraction (SP-SRF) on the integrity, stability, and peroxidation of sperm membranes after thawing. Each ejaculate (n = 24) was divided among 4 treatments: control (CT), centrifuged and suspended in autologous SP-SRF (CS), centrifuged with withdrawn SP-SRF (CW), and post-thawed SP arising from the whole sperm-rich fraction addition to CW (CWSP). After thawing, all treatments were incubated for 5, 60, and 120 min and were analyzed for membrane integrity, fluidity, and peroxidation by flow cytometer. The absence of SP-SRF increased the lipid disorder (P < 0.05) but had no effect on lipid peroxidation (P > 0.05) or membrane integrity (P > 0.05). However, the increase in lipid disorder by withdrawal of SP-SRF was reversed by SP-SRF addition (P < 0.05) to the post-thawing medium, whereas plasma and acrosomal membrane integrity (P > 0.05) and lipid peroxidation (P > 0.05) were unchanged. In conclusion, despite the centrifugation effects, the addition of SP arising from the whole sperm-rich fraction to post-thawed boar semen decreased sperm lipid disorder without an influence of the sperm membrane integrity and peroxidation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-11-26T16:32:57Z 2018-11-26T16:32:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2527/jas2016-0293 Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 5, p. 1906-1912, 2016. 0021-8812 http://hdl.handle.net/11449/161494 10.2527/jas2016-0293 WOS:000375783300014 WOS000375783300014.pdf |
url |
http://dx.doi.org/10.2527/jas2016-0293 http://hdl.handle.net/11449/161494 |
identifier_str_mv |
Journal Of Animal Science. Champaign: Amer Soc Animal Science, v. 94, n. 5, p. 1906-1912, 2016. 0021-8812 10.2527/jas2016-0293 WOS:000375783300014 WOS000375783300014.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Animal Science 0,848 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1906-1912 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Animal Science |
publisher.none.fl_str_mv |
Amer Soc Animal Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1826304516445700096 |