Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11694-022-01565-1 http://hdl.handle.net/11449/242142 |
Resumo: | Five protease-producing and non-mycotoxin-producing fungi (Mucor subtilissimus URM 4133, Mucor sp. URM 4146, Mucor guilliermondii URM 5848, Aspergillus viride-nutans URM 6629 and Penicillium decumbens URM 6018) were used for hydrolysis of caprine casein. Peptides obtained from different fungi were separated on two fractions: molecular mass (MM) < 3 kDa and MM from 3 to 10 kDa, and the peptide fractions were investigated for antimicrobial, antioxidant and antihypertensive bioactive properties. All the 3 to 10 kDa fractions of all fungi were able to inhibit the growth of the three Gram-negative bacteria and the hydrolysate from URM 5848 inhibited all bacteria, except the bacteria Gram-positive Enterococcus faecalis. All hydrolysates with the peptides between 3 and 10 kDa possessed a strong scavenging capacity for ABTS•+ [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals, in which URM 5848 (100% and 32%) and URM 4133 (99% and 29%), respectively for (ABTS•+ and DPPH) were the best hydrolysates. The antihypertensive activity was only observed for hydrolysates from the URM 5848 and URM 4133 fungi, and the higher inhibitory effect was observed on fractions < 3 kDa with URM 5848, 19% and URM 4133, 16%. This work was successful in demonstrating the hydrolysates from goat casein by selected fungal proteases are effective in producing different bioactive peptides. |
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Repositório Institucional da UNESP |
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Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysatesACE inhibitory activityAntimicrobial activityAntioxidant activityBioactive peptidesFungi proteaseGoat caseinFive protease-producing and non-mycotoxin-producing fungi (Mucor subtilissimus URM 4133, Mucor sp. URM 4146, Mucor guilliermondii URM 5848, Aspergillus viride-nutans URM 6629 and Penicillium decumbens URM 6018) were used for hydrolysis of caprine casein. Peptides obtained from different fungi were separated on two fractions: molecular mass (MM) < 3 kDa and MM from 3 to 10 kDa, and the peptide fractions were investigated for antimicrobial, antioxidant and antihypertensive bioactive properties. All the 3 to 10 kDa fractions of all fungi were able to inhibit the growth of the three Gram-negative bacteria and the hydrolysate from URM 5848 inhibited all bacteria, except the bacteria Gram-positive Enterococcus faecalis. All hydrolysates with the peptides between 3 and 10 kDa possessed a strong scavenging capacity for ABTS•+ [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals, in which URM 5848 (100% and 32%) and URM 4133 (99% and 29%), respectively for (ABTS•+ and DPPH) were the best hydrolysates. The antihypertensive activity was only observed for hydrolysates from the URM 5848 and URM 4133 fungi, and the higher inhibitory effect was observed on fractions < 3 kDa with URM 5848, 19% and URM 4133, 16%. This work was successful in demonstrating the hydrolysates from goat casein by selected fungal proteases are effective in producing different bioactive peptides.Institute of Biosciences Humanities and Exact Sciences (IBILCE) São Paulo State University (UNESP), Cristóvão Colombo Street, 2265, Jardim Nazareth, Campus São José do Rio PretoLaboratory of Microbial Ecology and Biotechnology Department of Biology Federal University of Ceará (UFC), Humberto Montel Avenue, CearáDepartment of Mycology Center of Biosciences Federal University of Pernambuco (UFPE), Prof. Nelson Chaves Avenue, PernambucoDepartment of Statistics and Applied Mathematics Federal University of Ceará (UFC)Laboratory of Microbiology Enzymatic Technology and Bioproducts Federal University of Agreste of Pernambuco (UFAPE), Bom Pastor Avenue, PernambucoCentro de Biotecnologia e Química Fina (CBQF) Escola Superior de Biotecnologia Universidade Católica Portuguesa (UCP), Rua Arquiteto Lobão Vital, 172Institute of Biosciences Humanities and Exact Sciences (IBILCE) São Paulo State University (UNESP), Cristóvão Colombo Street, 2265, Jardim Nazareth, Campus São José do Rio PretoUniversidade Estadual Paulista (UNESP)Federal University of Ceará (UFC)Universidade Federal de Pernambuco (UFPE)Federal University of Agreste of Pernambuco (UFAPE)Universidade Católica Portuguesa (UCP)Gomes, José Erick Galindo [UNESP]da Silva Nascimento, Talita Camila Evaristode Souza-Motta, Cristina MariaMontalvo, Gualberto Segundo AgamezBoscolo, Mauricio [UNESP]Gomes, Eleni [UNESP]Moreira, Keila AparecidaPintado, Maria Manuelada Silva, Roberto [UNESP]2023-03-02T10:06:50Z2023-03-02T10:06:50Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11694-022-01565-1Journal of Food Measurement and Characterization.2193-41342193-4126http://hdl.handle.net/11449/24214210.1007/s11694-022-01565-12-s2.0-85135847371Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Food Measurement and Characterizationinfo:eu-repo/semantics/openAccess2023-03-02T10:06:50Zoai:repositorio.unesp.br:11449/242142Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T10:06:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
title |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
spellingShingle |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates Gomes, José Erick Galindo [UNESP] ACE inhibitory activity Antimicrobial activity Antioxidant activity Bioactive peptides Fungi protease Goat casein |
title_short |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
title_full |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
title_fullStr |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
title_full_unstemmed |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
title_sort |
Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates |
author |
Gomes, José Erick Galindo [UNESP] |
author_facet |
Gomes, José Erick Galindo [UNESP] da Silva Nascimento, Talita Camila Evaristo de Souza-Motta, Cristina Maria Montalvo, Gualberto Segundo Agamez Boscolo, Mauricio [UNESP] Gomes, Eleni [UNESP] Moreira, Keila Aparecida Pintado, Maria Manuela da Silva, Roberto [UNESP] |
author_role |
author |
author2 |
da Silva Nascimento, Talita Camila Evaristo de Souza-Motta, Cristina Maria Montalvo, Gualberto Segundo Agamez Boscolo, Mauricio [UNESP] Gomes, Eleni [UNESP] Moreira, Keila Aparecida Pintado, Maria Manuela da Silva, Roberto [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Ceará (UFC) Universidade Federal de Pernambuco (UFPE) Federal University of Agreste of Pernambuco (UFAPE) Universidade Católica Portuguesa (UCP) |
dc.contributor.author.fl_str_mv |
Gomes, José Erick Galindo [UNESP] da Silva Nascimento, Talita Camila Evaristo de Souza-Motta, Cristina Maria Montalvo, Gualberto Segundo Agamez Boscolo, Mauricio [UNESP] Gomes, Eleni [UNESP] Moreira, Keila Aparecida Pintado, Maria Manuela da Silva, Roberto [UNESP] |
dc.subject.por.fl_str_mv |
ACE inhibitory activity Antimicrobial activity Antioxidant activity Bioactive peptides Fungi protease Goat casein |
topic |
ACE inhibitory activity Antimicrobial activity Antioxidant activity Bioactive peptides Fungi protease Goat casein |
description |
Five protease-producing and non-mycotoxin-producing fungi (Mucor subtilissimus URM 4133, Mucor sp. URM 4146, Mucor guilliermondii URM 5848, Aspergillus viride-nutans URM 6629 and Penicillium decumbens URM 6018) were used for hydrolysis of caprine casein. Peptides obtained from different fungi were separated on two fractions: molecular mass (MM) < 3 kDa and MM from 3 to 10 kDa, and the peptide fractions were investigated for antimicrobial, antioxidant and antihypertensive bioactive properties. All the 3 to 10 kDa fractions of all fungi were able to inhibit the growth of the three Gram-negative bacteria and the hydrolysate from URM 5848 inhibited all bacteria, except the bacteria Gram-positive Enterococcus faecalis. All hydrolysates with the peptides between 3 and 10 kDa possessed a strong scavenging capacity for ABTS•+ [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals, in which URM 5848 (100% and 32%) and URM 4133 (99% and 29%), respectively for (ABTS•+ and DPPH) were the best hydrolysates. The antihypertensive activity was only observed for hydrolysates from the URM 5848 and URM 4133 fungi, and the higher inhibitory effect was observed on fractions < 3 kDa with URM 5848, 19% and URM 4133, 16%. This work was successful in demonstrating the hydrolysates from goat casein by selected fungal proteases are effective in producing different bioactive peptides. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T10:06:50Z 2023-03-02T10:06:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11694-022-01565-1 Journal of Food Measurement and Characterization. 2193-4134 2193-4126 http://hdl.handle.net/11449/242142 10.1007/s11694-022-01565-1 2-s2.0-85135847371 |
url |
http://dx.doi.org/10.1007/s11694-022-01565-1 http://hdl.handle.net/11449/242142 |
identifier_str_mv |
Journal of Food Measurement and Characterization. 2193-4134 2193-4126 10.1007/s11694-022-01565-1 2-s2.0-85135847371 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Food Measurement and Characterization |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965442425487360 |