Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans

Detalhes bibliográficos
Autor(a) principal: Corrêa, Camila R. [UNESP]
Data de Publicação: 2011
Outros Autores: Dias-Melicio, Luciane A. [UNESP], Calvi, Sueli A. [UNESP], Lastária, Sidney [UNESP], Soares, Angela M.V.C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1476-9255-8-23
http://hdl.handle.net/11449/226490
Resumo: Background: Arterial peripheral disease is a condition caused by the blocked blood flow resulting from arterial cholesterol deposits within the arms, legs and aorta. Studies have shown that macrophages in atherosclerotic plaque are highly activated, which makes these cells important antigen-presenting cells that develop a specific immune response, in which LDLox is the inducing antigen. As functional changes of cells which participate in the atherogenesis process may occur in the peripheral blood, the objectives of the present study were to evaluate plasma levels of anti-inflammatory and inflammatory cytokines including TNF-, IFN-, interleukin-6 (IL-6), IL-10 and TGF- in patients with peripheral arteriosclerosis obliterans, to assess the monocyte activation level in peripheral blood through the ability of these cells to release hydrogen peroxide (H2O2) and to develop fungicidal activity against Candida albicans (C. albicans) in vitro. Methods. TNF-, IFN-, IL-6, IL-10 and TGF- from plasma of patients were detected by ELISA. Monocyte cultures activated in vitro with TNF-alpha and IFN-gamma were evaluated by fungicidal activity against C. albicans by culture plating and Colony Forming Unit (CFU) recovery, and by H2O2production. Results: Plasma levels of all cytokines were significantly higher in patients compared to those detected in control subjects. Control group monocytes did not release substantial levels of H2O2 in vitro, but these levels were significantly increased after activation with IFN- and TNF-. Monocytes of patients, before and after activation, responded less than those of control subjects. Similar results were found when fungicidal activity was evaluated. The results seen in patients were always significantly smaller than among control subjects. Conclusions:The results revealed an unresponsiveness of patient monocytes in vitro probably due to the high activation process occurring in vivo as corroborated by high plasma cytokine levels. © 2011 Corrêa et al; licensee BioMed Central Ltd.
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spelling Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliteranscytokinesmonocytesPeripheral arteriosclerosis obliteransperipheral bloodBackground: Arterial peripheral disease is a condition caused by the blocked blood flow resulting from arterial cholesterol deposits within the arms, legs and aorta. Studies have shown that macrophages in atherosclerotic plaque are highly activated, which makes these cells important antigen-presenting cells that develop a specific immune response, in which LDLox is the inducing antigen. As functional changes of cells which participate in the atherogenesis process may occur in the peripheral blood, the objectives of the present study were to evaluate plasma levels of anti-inflammatory and inflammatory cytokines including TNF-, IFN-, interleukin-6 (IL-6), IL-10 and TGF- in patients with peripheral arteriosclerosis obliterans, to assess the monocyte activation level in peripheral blood through the ability of these cells to release hydrogen peroxide (H2O2) and to develop fungicidal activity against Candida albicans (C. albicans) in vitro. Methods. TNF-, IFN-, IL-6, IL-10 and TGF- from plasma of patients were detected by ELISA. Monocyte cultures activated in vitro with TNF-alpha and IFN-gamma were evaluated by fungicidal activity against C. albicans by culture plating and Colony Forming Unit (CFU) recovery, and by H2O2production. Results: Plasma levels of all cytokines were significantly higher in patients compared to those detected in control subjects. Control group monocytes did not release substantial levels of H2O2 in vitro, but these levels were significantly increased after activation with IFN- and TNF-. Monocytes of patients, before and after activation, responded less than those of control subjects. Similar results were found when fungicidal activity was evaluated. The results seen in patients were always significantly smaller than among control subjects. Conclusions:The results revealed an unresponsiveness of patient monocytes in vitro probably due to the high activation process occurring in vivo as corroborated by high plasma cytokine levels. © 2011 Corrêa et al; licensee BioMed Central Ltd.Departamento de Patologia UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Doenças Tropicais e Diagnástico Por Imagem UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Cirurgia e Ortopedia UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Microbiologia e Imunologia UNESP - Univ Estadual Paulista Instituto de Biociências, CEP 18618-970, SPDepartamento de Patologia UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Doenças Tropicais e Diagnástico Por Imagem UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Cirurgia e Ortopedia UNESP - Univ Estadual Paulista Faculdade de Medicina, CEP 18618-970, SPDepartamento de Microbiologia e Imunologia UNESP - Univ Estadual Paulista Instituto de Biociências, CEP 18618-970, SPUniversidade Estadual Paulista (UNESP)Corrêa, Camila R. [UNESP]Dias-Melicio, Luciane A. [UNESP]Calvi, Sueli A. [UNESP]Lastária, Sidney [UNESP]Soares, Angela M.V.C. [UNESP]2022-04-29T00:13:44Z2022-04-29T00:13:44Z2011-08-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/1476-9255-8-23Journal of Inflammation, v. 8.1476-9255http://hdl.handle.net/11449/22649010.1186/1476-9255-8-232-s2.0-80052103627Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Inflammationinfo:eu-repo/semantics/openAccess2024-09-03T13:14:53Zoai:repositorio.unesp.br:11449/226490Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
title Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
spellingShingle Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
Corrêa, Camila R. [UNESP]
cytokines
monocytes
Peripheral arteriosclerosis obliterans
peripheral blood
title_short Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
title_full Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
title_fullStr Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
title_full_unstemmed Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
title_sort Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans
author Corrêa, Camila R. [UNESP]
author_facet Corrêa, Camila R. [UNESP]
Dias-Melicio, Luciane A. [UNESP]
Calvi, Sueli A. [UNESP]
Lastária, Sidney [UNESP]
Soares, Angela M.V.C. [UNESP]
author_role author
author2 Dias-Melicio, Luciane A. [UNESP]
Calvi, Sueli A. [UNESP]
Lastária, Sidney [UNESP]
Soares, Angela M.V.C. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Corrêa, Camila R. [UNESP]
Dias-Melicio, Luciane A. [UNESP]
Calvi, Sueli A. [UNESP]
Lastária, Sidney [UNESP]
Soares, Angela M.V.C. [UNESP]
dc.subject.por.fl_str_mv cytokines
monocytes
Peripheral arteriosclerosis obliterans
peripheral blood
topic cytokines
monocytes
Peripheral arteriosclerosis obliterans
peripheral blood
description Background: Arterial peripheral disease is a condition caused by the blocked blood flow resulting from arterial cholesterol deposits within the arms, legs and aorta. Studies have shown that macrophages in atherosclerotic plaque are highly activated, which makes these cells important antigen-presenting cells that develop a specific immune response, in which LDLox is the inducing antigen. As functional changes of cells which participate in the atherogenesis process may occur in the peripheral blood, the objectives of the present study were to evaluate plasma levels of anti-inflammatory and inflammatory cytokines including TNF-, IFN-, interleukin-6 (IL-6), IL-10 and TGF- in patients with peripheral arteriosclerosis obliterans, to assess the monocyte activation level in peripheral blood through the ability of these cells to release hydrogen peroxide (H2O2) and to develop fungicidal activity against Candida albicans (C. albicans) in vitro. Methods. TNF-, IFN-, IL-6, IL-10 and TGF- from plasma of patients were detected by ELISA. Monocyte cultures activated in vitro with TNF-alpha and IFN-gamma were evaluated by fungicidal activity against C. albicans by culture plating and Colony Forming Unit (CFU) recovery, and by H2O2production. Results: Plasma levels of all cytokines were significantly higher in patients compared to those detected in control subjects. Control group monocytes did not release substantial levels of H2O2 in vitro, but these levels were significantly increased after activation with IFN- and TNF-. Monocytes of patients, before and after activation, responded less than those of control subjects. Similar results were found when fungicidal activity was evaluated. The results seen in patients were always significantly smaller than among control subjects. Conclusions:The results revealed an unresponsiveness of patient monocytes in vitro probably due to the high activation process occurring in vivo as corroborated by high plasma cytokine levels. © 2011 Corrêa et al; licensee BioMed Central Ltd.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-31
2022-04-29T00:13:44Z
2022-04-29T00:13:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1476-9255-8-23
Journal of Inflammation, v. 8.
1476-9255
http://hdl.handle.net/11449/226490
10.1186/1476-9255-8-23
2-s2.0-80052103627
url http://dx.doi.org/10.1186/1476-9255-8-23
http://hdl.handle.net/11449/226490
identifier_str_mv Journal of Inflammation, v. 8.
1476-9255
10.1186/1476-9255-8-23
2-s2.0-80052103627
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Inflammation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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