Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992005000300010 http://hdl.handle.net/11449/211858 |
Resumo: | Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom. |
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Repositório Institucional da UNESP |
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Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenomingBothrops jararaca venomovine antiserumvenom levelsedemaELISAAlternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom.Universidade de São Paulo, Institute of PhysicsUniversidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Institute of BiosciencesCentro de Estudos de Venenos e Animais PeçonhentosUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ferreira, J.Bastos, M. F. [UNESP]Pelizon, A. C. [UNESP]Peres, C. M. [UNESP]Cavalcante, D. P. [UNESP]Sartori, A. [UNESP]2021-07-14T10:30:44Z2021-07-14T10:30:44Z2005-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article336-349application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000300010Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 3, p. 336-349, 2005.1678-9199http://hdl.handle.net/11449/21185810.1590/S1678-91992005000300010S1678-91992005000300010S1678-91992005000300010.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-11-21T06:16:12Zoai:repositorio.unesp.br:11449/211858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:23:05.369769Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
spellingShingle |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming Ferreira, J. Bothrops jararaca venom ovine antiserum venom levels edema ELISA |
title_short |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_full |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_fullStr |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_full_unstemmed |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_sort |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
author |
Ferreira, J. |
author_facet |
Ferreira, J. Bastos, M. F. [UNESP] Pelizon, A. C. [UNESP] Peres, C. M. [UNESP] Cavalcante, D. P. [UNESP] Sartori, A. [UNESP] |
author_role |
author |
author2 |
Bastos, M. F. [UNESP] Pelizon, A. C. [UNESP] Peres, C. M. [UNESP] Cavalcante, D. P. [UNESP] Sartori, A. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferreira, J. Bastos, M. F. [UNESP] Pelizon, A. C. [UNESP] Peres, C. M. [UNESP] Cavalcante, D. P. [UNESP] Sartori, A. [UNESP] |
dc.subject.por.fl_str_mv |
Bothrops jararaca venom ovine antiserum venom levels edema ELISA |
topic |
Bothrops jararaca venom ovine antiserum venom levels edema ELISA |
description |
Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09 2021-07-14T10:30:44Z 2021-07-14T10:30:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992005000300010 Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 3, p. 336-349, 2005. 1678-9199 http://hdl.handle.net/11449/211858 10.1590/S1678-91992005000300010 S1678-91992005000300010 S1678-91992005000300010.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992005000300010 http://hdl.handle.net/11449/211858 |
identifier_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 3, p. 336-349, 2005. 1678-9199 10.1590/S1678-91992005000300010 S1678-91992005000300010 S1678-91992005000300010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
336-349 application/pdf |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128925349445632 |