Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1365-2567.2004.01931.x http://hdl.handle.net/11449/67846 |
Resumo: | A DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed the question of which protective mechanisms are activated in Mycobacterium tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation of the cellular immune response in the lungs of infected mice 30 days after infection (initiation of immune therapy) and in those of uninfected mice. After 70 days (end of immune therapy), the immune responses of infected untreated mice, infected pHSP65-treated mice and infected pCDNA3-treated mice were also evaluated. Our results show that the most significant effect of pHSP65 was the stimulation of CD8+ lung cell activation, interferon-γ recovery and reduction of lung injury. There was also partial restoration of the production of tumour necrosis factor-α. Treatment with pcDNA3 vector also induced an immune stimulatory effect. However, only infected pHSP65-treated mice were able to produce significant levels of interferon-γ and to restrict the growth of bacilli. |
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Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injuryInterferon-γMycobacterium tuberculosispHSP65 DNA therapyProtectionT CD8+ lymphocytesCD8 antigenDNA vaccinegamma interferonheat shock proteinsynaptotagminanimal experimentanimal modelanimal tissuecell activationcellular immunitycontrolled studydrug effectdrug mechanismevaluationfemaleimmune responseimmunoregulationimmunotherapylung injurylung tuberculosismousenonhumanpriority journaltreatment outcomeAnimalsAntigens, CD18Antigens, CD28Antigens, CD95Bacterial ProteinsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesChaperoninsFas Ligand ProteinFemaleInterferon Type IILymphocyte ActivationMembrane GlycoproteinsMiceMice, Inbred BALB CTuberculosis, PulmonaryUp-RegulationVaccines, DNAA DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed the question of which protective mechanisms are activated in Mycobacterium tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation of the cellular immune response in the lungs of infected mice 30 days after infection (initiation of immune therapy) and in those of uninfected mice. After 70 days (end of immune therapy), the immune responses of infected untreated mice, infected pHSP65-treated mice and infected pCDNA3-treated mice were also evaluated. Our results show that the most significant effect of pHSP65 was the stimulation of CD8+ lung cell activation, interferon-γ recovery and reduction of lung injury. There was also partial restoration of the production of tumour necrosis factor-α. Treatment with pcDNA3 vector also induced an immune stimulatory effect. However, only infected pHSP65-treated mice were able to produce significant levels of interferon-γ and to restrict the growth of bacilli.TB Network Dept. of Biochemistry and Immunology University of São PauloDepartment of Pathology Ribeirão Preto Sch. of Med. University of São PauloDept. of Microbiology and Immunology Biosciences Institute São Paulo State UniversityDept. of Biochemistry and Immunology Ribeirão Preto Sch. of Med. University of São Paulo, Avenida Bandeirantes, 3900, 14049-900, Ribeirão Preto, SPDept. of Microbiology and Immunology Biosciences Institute São Paulo State UniversityUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Bonato, V. L DGonçalves, E. D CSoares, E. G.Santos, R. R.Sartori, A. [UNESP]Coelho-Castelo, A. A MSilva, C. L.2014-05-27T11:21:08Z2014-05-27T11:21:08Z2004-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article130-138application/pdfhttp://dx.doi.org/10.1111/j.1365-2567.2004.01931.xImmunology, v. 113, n. 1, p. 130-138, 2004.0019-2805http://hdl.handle.net/11449/6784610.1111/j.1365-2567.2004.01931.x2-s2.0-44442719052-s2.0-4444271905.pdf4977572416129527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengImmunology3.3581,690info:eu-repo/semantics/openAccess2024-01-08T06:25:56Zoai:repositorio.unesp.br:11449/67846Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T20:08:39.887141Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
title |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
spellingShingle |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury Bonato, V. L D Interferon-γ Mycobacterium tuberculosis pHSP65 DNA therapy Protection T CD8+ lymphocytes CD8 antigen DNA vaccine gamma interferon heat shock protein synaptotagmin animal experiment animal model animal tissue cell activation cellular immunity controlled study drug effect drug mechanism evaluation female immune response immunoregulation immunotherapy lung injury lung tuberculosis mouse nonhuman priority journal treatment outcome Animals Antigens, CD18 Antigens, CD28 Antigens, CD95 Bacterial Proteins CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Chaperonins Fas Ligand Protein Female Interferon Type II Lymphocyte Activation Membrane Glycoproteins Mice Mice, Inbred BALB C Tuberculosis, Pulmonary Up-Regulation Vaccines, DNA |
title_short |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
title_full |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
title_fullStr |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
title_full_unstemmed |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
title_sort |
Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: Activation of CD8+ cells, interferon-γ recovery and reduction of lung injury |
author |
Bonato, V. L D |
author_facet |
Bonato, V. L D Gonçalves, E. D C Soares, E. G. Santos, R. R. Sartori, A. [UNESP] Coelho-Castelo, A. A M Silva, C. L. |
author_role |
author |
author2 |
Gonçalves, E. D C Soares, E. G. Santos, R. R. Sartori, A. [UNESP] Coelho-Castelo, A. A M Silva, C. L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bonato, V. L D Gonçalves, E. D C Soares, E. G. Santos, R. R. Sartori, A. [UNESP] Coelho-Castelo, A. A M Silva, C. L. |
dc.subject.por.fl_str_mv |
Interferon-γ Mycobacterium tuberculosis pHSP65 DNA therapy Protection T CD8+ lymphocytes CD8 antigen DNA vaccine gamma interferon heat shock protein synaptotagmin animal experiment animal model animal tissue cell activation cellular immunity controlled study drug effect drug mechanism evaluation female immune response immunoregulation immunotherapy lung injury lung tuberculosis mouse nonhuman priority journal treatment outcome Animals Antigens, CD18 Antigens, CD28 Antigens, CD95 Bacterial Proteins CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Chaperonins Fas Ligand Protein Female Interferon Type II Lymphocyte Activation Membrane Glycoproteins Mice Mice, Inbred BALB C Tuberculosis, Pulmonary Up-Regulation Vaccines, DNA |
topic |
Interferon-γ Mycobacterium tuberculosis pHSP65 DNA therapy Protection T CD8+ lymphocytes CD8 antigen DNA vaccine gamma interferon heat shock protein synaptotagmin animal experiment animal model animal tissue cell activation cellular immunity controlled study drug effect drug mechanism evaluation female immune response immunoregulation immunotherapy lung injury lung tuberculosis mouse nonhuman priority journal treatment outcome Animals Antigens, CD18 Antigens, CD28 Antigens, CD95 Bacterial Proteins CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Chaperonins Fas Ligand Protein Female Interferon Type II Lymphocyte Activation Membrane Glycoproteins Mice Mice, Inbred BALB C Tuberculosis, Pulmonary Up-Regulation Vaccines, DNA |
description |
A DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed the question of which protective mechanisms are activated in Mycobacterium tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation of the cellular immune response in the lungs of infected mice 30 days after infection (initiation of immune therapy) and in those of uninfected mice. After 70 days (end of immune therapy), the immune responses of infected untreated mice, infected pHSP65-treated mice and infected pCDNA3-treated mice were also evaluated. Our results show that the most significant effect of pHSP65 was the stimulation of CD8+ lung cell activation, interferon-γ recovery and reduction of lung injury. There was also partial restoration of the production of tumour necrosis factor-α. Treatment with pcDNA3 vector also induced an immune stimulatory effect. However, only infected pHSP65-treated mice were able to produce significant levels of interferon-γ and to restrict the growth of bacilli. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 2014-05-27T11:21:08Z 2014-05-27T11:21:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1365-2567.2004.01931.x Immunology, v. 113, n. 1, p. 130-138, 2004. 0019-2805 http://hdl.handle.net/11449/67846 10.1111/j.1365-2567.2004.01931.x 2-s2.0-4444271905 2-s2.0-4444271905.pdf 4977572416129527 |
url |
http://dx.doi.org/10.1111/j.1365-2567.2004.01931.x http://hdl.handle.net/11449/67846 |
identifier_str_mv |
Immunology, v. 113, n. 1, p. 130-138, 2004. 0019-2805 10.1111/j.1365-2567.2004.01931.x 2-s2.0-4444271905 2-s2.0-4444271905.pdf 4977572416129527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Immunology 3.358 1,690 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
130-138 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045652077215744 |