Rabies laboratory diagnosis: Peculiar features of samples from equine origin
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/195701 |
Resumo: | Rabies laboratory diagnosis is performed by using microscopic examination for Negri bodies (MEN), fluorescent-antibody test (FAT) and mouse inoculation test (MIT). In the majority of cases, when specimens are properly collected and conserved and the laboratory worker has good experience, agreement among employed techniques is verified. Comparing the sensitivity of these three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from bovine (983 positives) and 703 from equine (Ill positives) species, it was observed that in equine rabid samples, this agreement was not maintained. For the latter specie, only in few opportunities the Negri bodies could be observed. With respect to FAT, the test detected a lower percentage of positive equine samples compared to bovine species. Statistical analysis demonstrated that the difference was significative. Mouse inoculation test proved to be more sensitive. However, a significant difference in mice incubation period was observed for samples from both species. The absence of inclusion bodies and the longer incubation period for equine samples suggest that rabies pathogenesis studies for equine species have to be intensified. |
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Repositório Institucional da UNESP |
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Rabies laboratory diagnosis: Peculiar features of samples from equine originequine rabieslaboratory diagnosisRabies laboratory diagnosis is performed by using microscopic examination for Negri bodies (MEN), fluorescent-antibody test (FAT) and mouse inoculation test (MIT). In the majority of cases, when specimens are properly collected and conserved and the laboratory worker has good experience, agreement among employed techniques is verified. Comparing the sensitivity of these three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from bovine (983 positives) and 703 from equine (Ill positives) species, it was observed that in equine rabid samples, this agreement was not maintained. For the latter specie, only in few opportunities the Negri bodies could be observed. With respect to FAT, the test detected a lower percentage of positive equine samples compared to bovine species. Statistical analysis demonstrated that the difference was significative. Mouse inoculation test proved to be more sensitive. However, a significant difference in mice incubation period was observed for samples from both species. The absence of inclusion bodies and the longer incubation period for equine samples suggest that rabies pathogenesis studies for equine species have to be intensified.Inst Biol, Lab Raiva & Encefalites Virais, Secao Raiva & Encefalomielite, BR-04014001 Sao Paulo, BrazilInst Biol, Lab Reg Pindamonhangaba, Sao Paulo, BrazilUniv Estadual Paulista, Dept Apoio Prod & Saude Anim, Aracatuba, SP, BrazilUniv Sao Paulo, Fac Saude Publ, Sao Paulo, BrazilUniv Estadual Paulista, Dept Apoio Prod & Saude Anim, Aracatuba, SP, BrazilSoc Brasileira MicrobiologiaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Peixoto, ZMPCunha, EMSSacramento, DRVSouza, MCAMSilva, LHQ daGermano, P. L.Kroeff, S. S.Kotait, I2020-12-10T18:00:39Z2020-12-10T18:00:39Z2000-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article72-75Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 31, n. 1, p. 72-75, 2000.1517-8382http://hdl.handle.net/11449/195701WOS:000165675900016Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T10:49:09Zoai:repositorio.unesp.br:11449/195701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:49:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
title |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
spellingShingle |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin Peixoto, ZMP equine rabies laboratory diagnosis |
title_short |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
title_full |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
title_fullStr |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
title_full_unstemmed |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
title_sort |
Rabies laboratory diagnosis: Peculiar features of samples from equine origin |
author |
Peixoto, ZMP |
author_facet |
Peixoto, ZMP Cunha, EMS Sacramento, DRV Souza, MCAM Silva, LHQ da Germano, P. L. Kroeff, S. S. Kotait, I |
author_role |
author |
author2 |
Cunha, EMS Sacramento, DRV Souza, MCAM Silva, LHQ da Germano, P. L. Kroeff, S. S. Kotait, I |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Peixoto, ZMP Cunha, EMS Sacramento, DRV Souza, MCAM Silva, LHQ da Germano, P. L. Kroeff, S. S. Kotait, I |
dc.subject.por.fl_str_mv |
equine rabies laboratory diagnosis |
topic |
equine rabies laboratory diagnosis |
description |
Rabies laboratory diagnosis is performed by using microscopic examination for Negri bodies (MEN), fluorescent-antibody test (FAT) and mouse inoculation test (MIT). In the majority of cases, when specimens are properly collected and conserved and the laboratory worker has good experience, agreement among employed techniques is verified. Comparing the sensitivity of these three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from bovine (983 positives) and 703 from equine (Ill positives) species, it was observed that in equine rabid samples, this agreement was not maintained. For the latter specie, only in few opportunities the Negri bodies could be observed. With respect to FAT, the test detected a lower percentage of positive equine samples compared to bovine species. Statistical analysis demonstrated that the difference was significative. Mouse inoculation test proved to be more sensitive. However, a significant difference in mice incubation period was observed for samples from both species. The absence of inclusion bodies and the longer incubation period for equine samples suggest that rabies pathogenesis studies for equine species have to be intensified. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 2020-12-10T18:00:39Z 2020-12-10T18:00:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 31, n. 1, p. 72-75, 2000. 1517-8382 http://hdl.handle.net/11449/195701 WOS:000165675900016 |
identifier_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 31, n. 1, p. 72-75, 2000. 1517-8382 WOS:000165675900016 |
url |
http://hdl.handle.net/11449/195701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
72-75 |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649303446552576 |