Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens

Detalhes bibliográficos
Autor(a) principal: Lima, Edna T.
Data de Publicação: 2007
Outros Autores: Andreatti Filho, Raphael Lucio [UNESP], Okamoto, Adriano S., Noujaim, Jose C., Barros, Mercia R., Crocci, Adalberto J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829185/
http://hdl.handle.net/11449/34196
Resumo: To determine the inhibitory capacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.
id UNSP_3ae3cf382346c571eea553f003b9630d
oai_identifier_str oai:repositorio.unesp.br:11449/34196
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickensTo determine the inhibitory capacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.São Paulo State Univ, Coll Vet Med & Anim Sci, Dept Clin Vet Med, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, Inst Biosci, Dept Biostat, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, Coll Vet Med & Anim Sci, Dept Clin Vet Med, BR-18618000 Botucatu, SP, BrazilSão Paulo State Univ, Inst Biosci, Dept Biostat, BR-18618000 Botucatu, SP, BrazilCanadian Vet Med AssocUniversidade Estadual Paulista (Unesp)Lima, Edna T.Andreatti Filho, Raphael Lucio [UNESP]Okamoto, Adriano S.Noujaim, Jose C.Barros, Mercia R.Crocci, Adalberto J.2014-05-20T15:23:24Z2014-05-20T15:23:24Z2007-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article103-107http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829185/Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 71, n. 2, p. 103-107, 2007.0830-9000http://hdl.handle.net/11449/34196WOS:00024563860000447039833802243730000-0002-0355-9841Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire0.9180,478info:eu-repo/semantics/openAccess2021-10-23T10:31:40Zoai:repositorio.unesp.br:11449/34196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:20:35.247050Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
spellingShingle Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
Lima, Edna T.
title_short Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_full Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_fullStr Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_full_unstemmed Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_sort Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
author Lima, Edna T.
author_facet Lima, Edna T.
Andreatti Filho, Raphael Lucio [UNESP]
Okamoto, Adriano S.
Noujaim, Jose C.
Barros, Mercia R.
Crocci, Adalberto J.
author_role author
author2 Andreatti Filho, Raphael Lucio [UNESP]
Okamoto, Adriano S.
Noujaim, Jose C.
Barros, Mercia R.
Crocci, Adalberto J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Lima, Edna T.
Andreatti Filho, Raphael Lucio [UNESP]
Okamoto, Adriano S.
Noujaim, Jose C.
Barros, Mercia R.
Crocci, Adalberto J.
description To determine the inhibitory capacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.
publishDate 2007
dc.date.none.fl_str_mv 2007-04-01
2014-05-20T15:23:24Z
2014-05-20T15:23:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829185/
Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 71, n. 2, p. 103-107, 2007.
0830-9000
http://hdl.handle.net/11449/34196
WOS:000245638600004
4703983380224373
0000-0002-0355-9841
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1829185/
http://hdl.handle.net/11449/34196
identifier_str_mv Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 71, n. 2, p. 103-107, 2007.
0830-9000
WOS:000245638600004
4703983380224373
0000-0002-0355-9841
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire
0.918
0,478
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 103-107
dc.publisher.none.fl_str_mv Canadian Vet Med Assoc
publisher.none.fl_str_mv Canadian Vet Med Assoc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128921916407808