Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens

Detalhes bibliográficos
Autor(a) principal: Lima, Edna T. [UNESP]
Data de Publicação: 2007
Outros Autores: Andreatti Filho, Raphael L. [UNESP], Okamoto, Adriano S. [UNESP], Noujaim, José C. [UNESP], Barros, Mércia R. [UNESP], Crocci, Adalberto J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224903
Resumo: To determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.
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spelling Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickensTo determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.Department of Clinical Veterinary Medicine College of Veterinary Medicine and Animal Science São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Biostatistics Institute for the Biosciences São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Clinical Veterinary Medicine College of Veterinary Medicine and Animal Science São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Biostatistics Institute for the Biosciences São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloUniversidade Estadual Paulista (UNESP)Lima, Edna T. [UNESP]Andreatti Filho, Raphael L. [UNESP]Okamoto, Adriano S. [UNESP]Noujaim, José C. [UNESP]Barros, Mércia R. [UNESP]Crocci, Adalberto J. [UNESP]2022-04-28T20:17:19Z2022-04-28T20:17:19Z2007-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article103-107Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007.0830-9000http://hdl.handle.net/11449/2249032-s2.0-34248328002Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Veterinary Researchinfo:eu-repo/semantics/openAccess2022-04-28T20:17:19Zoai:repositorio.unesp.br:11449/224903Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:26:17.729427Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
spellingShingle Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
Lima, Edna T. [UNESP]
title_short Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_full Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_fullStr Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_full_unstemmed Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
title_sort Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
author Lima, Edna T. [UNESP]
author_facet Lima, Edna T. [UNESP]
Andreatti Filho, Raphael L. [UNESP]
Okamoto, Adriano S. [UNESP]
Noujaim, José C. [UNESP]
Barros, Mércia R. [UNESP]
Crocci, Adalberto J. [UNESP]
author_role author
author2 Andreatti Filho, Raphael L. [UNESP]
Okamoto, Adriano S. [UNESP]
Noujaim, José C. [UNESP]
Barros, Mércia R. [UNESP]
Crocci, Adalberto J. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Lima, Edna T. [UNESP]
Andreatti Filho, Raphael L. [UNESP]
Okamoto, Adriano S. [UNESP]
Noujaim, José C. [UNESP]
Barros, Mércia R. [UNESP]
Crocci, Adalberto J. [UNESP]
description To determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.
publishDate 2007
dc.date.none.fl_str_mv 2007-04-01
2022-04-28T20:17:19Z
2022-04-28T20:17:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007.
0830-9000
http://hdl.handle.net/11449/224903
2-s2.0-34248328002
identifier_str_mv Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007.
0830-9000
2-s2.0-34248328002
url http://hdl.handle.net/11449/224903
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canadian Journal of Veterinary Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 103-107
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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